Adeno-associated virus vectors
Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... minute virus of mice, have been conducted. Minute virus of mice was reported to bind to both specialized (coated pits) and unspecified regions of the cell membrane, and ultrastructural studies have ...
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Differential Activation of Innate Immune Responses by Adenovirus and Adeno-Associated Virus Vectors
... adenovirus vectors prompted us to embark on the studies in this report ...AAV vectors induced the expressions of various chemokines in two epithelium-derived cell lines, REC and ...of adeno- ...
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Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors
... rAAV5 vectors containing smCBA-mCherry in Nrl-GFP mice, mCherry expression was docu- mented using a Micron III fundoscope (Phoenix Research Laboratories, Pleasanton, CA) with a red fluorescent ...T-V) ...
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Transduction by adeno-associated virus vectors in the rabbit airway: efficiency, persistence, and readministration.
... Transduction due to a second administration of AAV vector was completely inhibited, and this effect correlated with the production of neutralizing antibodies. The neutralizing activity was directed against the ...
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Transduction of rat pancreatic islets with pseudotyped adeno associated virus vectors
... AAV2 vectors with an AAV2 capsid (AAV2/2) to transduce effi- ciently mouse or human islets ...AAV2 vectors pseudotyped with the capsids of AAV1 (AAV2/1) [13,16,17], AAV5 (AAV2/5) [12,13,15,16], and AAV8 ...
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Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors
... rAAV vectors is required for preclinical and clinical ...helper virus functions into cells to produce rAAV vectors has many ...PS virus to provide packaging and helper functions led to a ...
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CpG depleted adeno associated virus vectors evade immune detection
... Intramuscular gene trans- fer has proven to be espe- cially challenging due to adaptive immune responses toward AAV-associated antigens. These findings suggest the predisposition for an immunogenic envi- ronment ...
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Packaging Cells Based on Inducible Gene Amplification for the Production of Adeno-Associated Virus Vectors
... Plasmid DNA. Plasmids used in this study are shown in Fig. 1C. pUHG17-1 (15) and pCWRAP (11) were obtained from H. Bujard (University of Heidel- berg, Germany) and S. Chatterjee (City of Hope National Medical Center, ...
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Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus
... Recombinant adeno-associated virus (rAAV) vectors are promising alternative gene delivery systems, based on the de- fective and nonpathogenic parvovirus adeno-associated ...
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High-Fidelity Correction of Mutations at Multiple Chromosomal Positions by Adeno-Associated Virus Vectors
... Adeno-associated virus (AAV) is a single-stranded, linear DNA virus with a ...AAV vectors containing foreign DNA between the viral TRs can be packaged by rep and cap gene products ...
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Repeat Transduction in the Mouse Lung by Using Adeno-Associated Virus Vectors with Different Serotypes
... AAV vectors in neonatal or adult rabbit lungs declined dramatically within the course of the experiments (12, 14, 32), whereas AAV vector expression in the epithelia of the mouse lung persisted for 8 months, the ...
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Complement Is an Essential Component of the Immune Response to Adeno-Associated Virus Vectors
... AAV vectors did not display direct cofactor activity, suggesting that in contrast to HSV-1, the host com- plement regulatory factor H was likely required to cleave C3b associated with AAV and adenovirus ...
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Repeated Delivery of Adeno-Associated Virus Vectors to the Rabbit Airway
... Detection of GFP expression by FACS. Cells brushed from the approximate site of vector instillation were analyzed for GFP expression by FACS using a Becton Dickinson (Philadelphia, Pa.) flow cytometer. Bronchial ...
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Adeno-associated Virus Vectors Efficiently Transduce Mouse and Rabbit Sensory Neurons Coinfected with Herpes Simplex Virus 1 following Peripheral Inoculation
... AAV vectors transduced DRG sensory neurons ...the vectors enter the neu- rons at nerve termini and are trafficked to cell bodies using the same transport machinery as HSV-1, and this conclusion is sup- ...
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Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... simplex virus type 1 (HSV-1)-based amplicon vec- tors are bacterial plasmids that contain two HSV-1 cis ele- ments, an origin of DNA replication (ori), and a DNA pack- aging/cleavage signal ...helper virus ...
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Design and Packaging of Adeno-Associated Virus Gene Targeting Vectors
... corresponding vectors AAV-5⬘AP-Psh, AAV-5⬘AP-Bss, AAV- 3⬘AP-Bam, AAV-3⬘AP-(B/A), and AAV-3⬘AP-(E/A), ...leukemia virus long terminal repeat (LTR) driving expression of a functional AP gene, as well as a ...
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The state of the art of adeno associated virus based vectors in gene therapy
... Recombinant adeno-associated viral (rAAV) vectors have rapidly advanced to the forefront of gene therapy in the past ...this virus, which allows great improvement in AAV vectors ...
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M. S.
... Hendra virus and offers great potential for Henipavirus protection in humans as ...Vaccine vectors included in different studies are vascular stomatitis virus (VSV), Rabies virus (RABV), ...
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Integrating Adenovirus–Adeno-Associated Virus Hybrid Vectors Devoid of All Viral Genes
... hybrid vectors contained viral genes that ex- press potentially cytotoxic and immunogenic viral proteins in infected ...gutless vectors car- rying a hygromycin resistance transgene flanked by AAV ITRs was ...
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A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.
... Adeno-associated virus (AAV) is gaining acceptance as a vector to deliver genes into a wide variety of cell types (17, ...the virus, which cannot exceed 5,000 nucleotides. Most AAV ...
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