Adherent Cells
Electroporation of adherent cells with low sample volumes on a microscope stage
... into adherent cells on the stage of an inverted microscope while continuing to observe ...studying cells with limited numbers, such as primary cells, explanted directly from the donor ...
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Measuring surface geometry of adherent cells using oblique transillumination
... supporting adherent cells is observed using oblique transillumination and rotated in the horizontal plane using a stepping ...illuminated cells uniquely determine the relative height differe- nces ...
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Cytoskeletal anisotropy controls geometry and forces of adherent cells
... (Received 15 March 2017; revised manuscript received 6 July 2018; published 26 October 2018) We investigate the geometrical and mechanical properties of adherent cells characterized by a highly anisotropic ...
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Gene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells
... stem cells (MSCs) provide a novel option in cellular therapy and tissue ...evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were ...these ...
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Optimization of proximity ligation assay (PLA) for detection of protein interactions and fusion proteins in non-adherent cells: application to pre-B lymphocytes
... HEK293T cells were co-transfected with pLenti-CMV- Puro-DEST bearing Halotag-RUNX1 or Halotag-ETV6- RUNX1, pSPAX2 and pCMV-VSV-G for packaging using Lipofectamine 3000 Transfection Reagent (Thermo Fisher ...Nalm6 ...
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Distinct roles of BMP and LKB1/AMPK signalling impacting ovarian cancer spheroid biology
... EOC cells transit the peritoneal cavity in suspension, presents unique therapeutic challenges for treatment of advanced- stage ovarian ...non-adherent cells will provide insights into novel targets ...
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Identification of three accessory cell populations in human bone marrow with erythroid burst promoting properties
... T cells, or T cell/monocyte collaboration in the induction of differentiation of peripheral blood erythroid burst-forming units (BFU-E) in ...T cells and adherent cells are similar to those of ...
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Ability of Staphylococcus spp. Isolated from Meningitis Patients to Adhesion
... of adherent cells of S. epidermidis was 230 cells / field, ...62 cells / fields, whereas S. aureus was 3 cells / field, while in ...>300 cells / field, finally the number of ...
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Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells
... blood cells, lymphocytes, monocytes and granulocytes – were defined according to their forward and side scattering (Figure 2A) as done by other groups ...non-adherent cells (P < ...blood ...
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<em>In vitro</em> and <em>in vivo</em> effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection
... Adherent cells were trypsinized and suspension cells were single-cell resuspended and transferred to Eppendorf tubes in two aliquots and washed with phosphate-buffered saline ...EGFP-positive ...
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The combination of high-fat diet-induced obesity and chronic ulcerative colitis reciprocally exacerbates adipose tissue and colon inflammation
... those cells moving slower than the cells at a regular flux in a given ...rolling cells was mea- sured as the number of rolling cells passing by a given point in the venule per minute, with ...
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Hypoxia-NOTCH1-SOX2 signaling is important for maintaining cancer stem cells in ovarian cancer
... for adherent cells were purchased from Thermo Fisher Scientific ...sphere cells, culture plates with ultra-low attachment surface were purchased from Corning Life Sciences (Tewksbury, ...
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A multi-structural single cell model of force-induced interactions of cytoskeletal components
... tissues, adherent cells are constantly exposed to a variety of mechanical ...forces. Cells interact with their extracellular environment, from which they gather information that influences their ...
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Local adherent technique for transplanting mesenchymal stem cells as a potential treatment of cartilage defect
... nonadherent cells, and the adherent cells were cultured for 7 days as passage 0 without ...The cells were then trypsinized, harvested and resuspended to be used for ...these cells had ...
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A simple in vitro method for evaluating dendritic cell-based vaccinations
... (iDCs), adherent cells were cultured for 6 days in medium containing recombinant GM-CSF and IL-4 at a concentration of 20 ng/ mL ...the cells were matured in complete media supplemented with ...
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Mechanisms of immune activation of human immunodeficiency virus in monocytes/macrophages.
... To determine the population of T cells involved, T cells from uninfected donors were treated with anti-CD4 or anti-CD8 antibodies and complement and then added to washed adherent cells f[r] ...
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Fetal hemoglobin accumulation in vitro Effect of adherent mononuclear cells
... of adherent mononuclear cells in the culture promoted a striking increase in the relative amount of Hb F in each of 44 experiments with 14 ...erythroid cells, their mean hemoglobin content, or the ...
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Tracking the cellulolytic activity of Clostridium thermocellum biofilms
... sessile cells to patches of uniform coverage (Figure 5, low ...magnification). Adherent cells were observed on fibers as deep as 20 to 30 microns from the ...of cells lining the substratum. ...
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Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells
... conditions (Fig. 4a). On the other hand, RT-qPCR ana- lysis also revealed that differentiation markers, AR and its two responsive genes (PSA/KLK3 and KLK2), showed significant downregulation in LNCaP/VCaP-derived ...
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Transit time of leukocytes rolling through venules controls cytokine induced inflammatory cell recruitment in vivo
... velocities. Adherent cells were defined to be the leukocytes that were not moving for at least 30 ...of adherent cells were measured for each venule and expressed per unit area of inside ...
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