Affinity chromatography
Use of Aleuria alantia Lectin Affinity Chromatography to Enrich Candidate Biomarkers from the Urine of Patients with Bladder Cancer
... lectin affinity chromatography in studies of the urinary glycoproteome, however these studies have used broad specificity lectins (expected to capture most glycoproteins) rather than focussing on ...
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Expression of a prokaryotic P-type ATPase in E. coli plasma membranes and purification by Ni2+-affinity chromatography
... Therefore, we developed a heterologous expression system of the 6xHis-tagged Synechocystis ATPase in E. coli to prove the assumed calcium specificity of this P-ATPase. The purification of the engineered enzyme under ...
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Intein Mediated Protein Synthesis and its Purification by Ni- NTA Affinity Chromatography
... An intein mediated protein purification system was established to generate His-tag free protein with high level of purity. To achieve this a synthetic intein gene (~171 bp) was constructed and fused to a histidine ...
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AFFINITY CHROMATOGRAPHY PURIFICATION AND PARTIAL CHARACTERISATION OF D GALACTOSE BINDING LECTIN FROM SEEDS OF JATROPHA CURCAS L
... CONCLUSION: Jatropha curcas L. seed cake is reported to be a rich source of protein for animal feed due to the high content of protein. The removal of toxic protein like curing by single-step affinity ...
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Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites
... metaloxide affinity chromatography (MOAC) and selective ion trap mass ...liquid chromatography coupled to mass spectrometry, and (iv) identification of phosphorylation sites of these enriched ...
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Removing Substances from Blood by Affinity Chromatography: I REMOVING BILIRUBIN AND OTHER ALBUMIN BOUND SUBSTANCES FROM PLASMA AND BLOOD WITH ALBUMIN CONJUGATED AGAROSE BEADS
... Substances such as bilirubin that bind tightly to plasma proteins cannot readily be removed from blood. We describe here the use of affinity chromatography as a new approach to the removal of proteinbound ...
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A proteomic approach based on peptide affinity chromatography, 2-dimensional electrophoresis and mass spectrometry to identify multiprotein complexes interacting with membrane-bound receptors
... by affinity chromatography, we combined mass spectrometry with immunoblotting ...the affinity chromatography step showed that both CASK and Mint1 were recruited by the C-terminal extremity of ...
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Partial Purification of Cholinesterase from Pangasius pangasius using Affinity Chromatography
... procainamide-based affinity chromatography column produces acceptable partially purified enzyme adequate for the application in the rapid detection of carbamate and organophosphate (OP) at the large ...
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Purification of Mycobacterium bovis BCG Tokyo antigens by chromatofocusing, lectin-affinity chromatography, and hydrophobic interaction chromatography.
... lectin-affinity chromatography, and hydrophobic interaction chroma- tography resulted in a simple purification of protein antigens of Mycobacterium bovis BCG Tokyo culture ...liquid chromatography ...
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Removing Substances from Blood by Affinity Chromatography: II REMOVING BILIRUBIN FROM THE BLOOD OF JAUNDICED RATS BY HEMOPERFUSION OVER ALBUMIN CONJUGATED AGAROSE BEADS
... In the rats with biliary obstruction and the Gunn rats the flow rate through the extracorporeal circuit 1 cm8/ min in all studies, the average BR concentration before hemoperfusion 7.47 [r] ...
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Purification of equine infectious anemia virus antigen by affinity chromatography
... Group-specific antigen derived from equine infectious anemia EIA virus has been widely used for the diagnosis of EIA, especially in immunodiffusion tests 1, 6, 9, 10.. However, the antig[r] ...
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Isolation of the dengue virus envelope glycoprotein from membranes of infected cells by concanavalin A affinity chromatography.
... 3 mg of BHK cellular membrane protein per ml see 132 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The membranes isolated from type 2 dengue virus-infected BHK-21/15 [r] ...
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Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein and Peptide Separations under Metal Affinity Chromatography
... the affinity of water molecules towards oxygen atoms in the PEG chains is high enough to avoid protein interaction ...water affinity relies on the conformation of the poly- mers that offers two hydrogen ...
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Glycoprotein gp118 of varicella-zoster virus: purification by serial affinity chromatography.
... Lane A, Original detergent-solubilized ['4C]glucosamine-labeled VZV-infected cell extract 10,800 cpm; lane B, proteins unretarded by immobilized heparin 35,000 cpm; lane C, bound protein[r] ...
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Separation of Herpes Simplex Virus-Induced Antigens by Concanavalin A Affinity Chromatography
... Figure 3B shows the gel diffusion pattern obtained when anti-CP-1 serum was reacted against HSV-SAM, CP-1 antigen, antigen b from infected cells, and the group I fraction obtained from u[r] ...
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Application of Affinity Chromatography Combined with Capillary Electrophoresis or Mass Spectrometry in the Biochemical Analysis
... elute affinity bound analytes, including phosphopeptides, from affinity media prior to MS ...that affinity-bound phosphoprotein and peptide, such as human apotransferrin and phosphokemptide, could be ...
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Purification of Avian Myeloblastosis Virus DNA Polymerase by Affinity Chromatography on Polycytidylate-Agarose
... Planimetric measurements performed on gel scans of fractions containing peak enzyme obtained by using linear gradients of increasing KCl concentration indicate that the eluted polymerase[r] ...
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Thermo Scientific Chromatography Columns and Consumables Your world of. chromatography
... Increased Retention of Polar Analytes In typical reversed phase chromatography, the retention of an analyte is directly related to its hydrophobicity: the more hydrophobic the analyte, the longer its retention. ...
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ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-A NEW REVOLUTION IN LIQUID CHROMATOGRAPHY
... Liquid Chromatography” or "Ultra High Pressure Liquid ...faster chromatography or a combination of both or Increase sensitivity, due to sharper (narrower) and higher ...
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Cultural affinity and mortgage discrimination
... an affinity with white applicants and that the cutoff “qual- ity” is below the average creditworthiness of both groups, we would expect to see large lenders that sell most of their loans to the sec- ondary market ...
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