Agrobacterium-Mediated
Application of sonication assisted Agrobacterium mediated transformation in Chenopodium rubrum L
... Sonication-assisted Agrobacterium-mediated trans- formation (SAAT) as an efficient Agrobacterium-based transformation technology was recently reported by Trick and Finer ...of Agrobacterium to ...
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Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods
... genes into plant genome, and selection of transgenic plants (Wang et al. 2013). Pollen tube-mediated gene transformation can be done by three major methods including microinjection, direct drop of DNA to the ...
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High-throughput Agrobacterium-mediated barley transformation
... of Agrobacterium-mediated barley transformation, efficiencies of ...of Agrobacterium-mediated transformation of cere- als detailing the efficiencies for the different crops see ...
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The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium mediated transformation of indica rice (Oryza sativa L )
... effective Agrobacterium-mediated transformation system with high efficiency of plant regeneration in a relatively short period depends on the transfor- mation parameters that permit efficient T-DNA ...
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Optimization of factors affecting Agrobacterium mediated transformation in sugarcane through axillary buds with AVP1 gene against drought and salinity
... of Agrobacterium-mediated with other explants or callus requires regeneration of transformed cells which is genotype- dependent, painstaking, labor intensive, time consuming and complicated with somaclonal ...
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Agrobacterium Mediated Transformation of Maize (Zea mays L.)
... the Agrobacterium strain, and the need to identify and to supplement specific signal molecules for the vir genes induction during the co- cultivation period (acetosiryngone or sinapinic acid) ...on ...
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Agrobacterium-mediated transformation systems of Primula vulgaris
... via Agrobacterium-mediated DNA transfer into different plant tissues offers an alter- native route for the rapid analysis of transgene functions, which is also amenable to high-throughput analysis ...and ...
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High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
... In Agrobacterium- mediated transformation of different tobacco genotypes, each treatment consisted of three replicates with 15 explants per Petri ...
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Female reproductive system of Amaranthus as the target for Agrobacterium mediated transformation
... crops. Agrobacterium-mediated floral dip transformation has been developed and successfully applied in the popular model plant such as Arabidopsis thaliana [7] and Medicago truncatula ...
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An improved protocol for rapid and efficient agrobacterium mediated transformation of tomato for salt tolerance (solanum lycopersicum l )
... The transformation efficiency was evaluated for cotyledonory and hypocotyl explants using Agrobacterium mediated transformation. Both explants were wounded and infected with A.tumefaciens strain LBA 4404 ...
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EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).
... and Agrobacterium transformation protocol have been developed for the recalcitrant Malaysian indica rice, ...the Agrobacterium-mediated protocol revealed that Agrobacterium supervirulent ...
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The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation
... after Agrobacterium-mediated transformation (Salomon and Puchta, 1998), consistent with the higher frequency of transgene integration after X-ray treatment, known to induce DSBs (Leskov et ...
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Agrobacterium mediated Genetic Transformation in Cucumber (var Shital) as Influenced by Explant, Inoculation Time and Co cultivation Period
... experiment mediated by A. tumefaciens. The Agrobacterium mediated transformation system is historically the first successful plant transformation system, marking the breakthrough in plant genetic ...
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Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay
... Although enhancers can be predicted based on genome-wide datasets associated with open chromatin and other epigenomic features, the function of predicted enhancers needs to be experimentally validated. A trans- genic ...
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Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1
... remove Agrobacterium and then the calli were transferred to the selection medium containing ...the Agrobacterium mediated transformation of ...
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PEG mediated and Agrobacterium mediated transformation in the mycopathogen Verticillium fungicola
... Here PEG-mediated co-transformation of V. fungi- cola protoplasts using vectors conferring resistance to hygromycin B, GUS activity and GFP activity, is described. Transformation of V. fungicola to hygro- mycin B ...
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REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF WATERMELON
... supplimented with agar and phytagel individually or/and together were tested. Agrobacterium-mediated transformation system: The explants of watermelon cv Giza 1 and Giza 21 were co-cultivated with A. ...
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Efficient method for Agrobacterium-mediated genetic transformation of tobacco nodal segments
... The agrobacterium transformation method is the most widely used method of gene transfer available for higher plants (Bottino et ...also Agrobacterium strain ...of Agrobacterium (Nester et ...
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Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
... particular Agrobacterium strains to transform plant cells is defined by their chromosomal and plasmid genomes which between them must encode all the machinery necessary for attachment and ...The ...
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Evolution of Transgenic Technology in Cereal Crops: a Review Approach
... This review study reports a concise analysis of investigations on origin and progress of transgenic technology in major cereal crops which is the integral part of the cereals improvement programmes. Most genetic ...
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