Agrobacterium-mediated transient transformation
Agrobacterium-mediated transformation systems of Primula vulgaris
... biolistic transient expression experiments, we assessed different 35S-UidA cassettes, and expres- sion of the UidA reporter gene was visualised by a β-glucuronidase (GUS) ...Primula transformation and that ...
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Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars
... zero transient gus expression, while no GUS ? plant was obtained from the callus line that exhibited relatively high percentage of transient gus ...the transient data based on GUS histochemical assay ...
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Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
... higher transient expression levels but lower regeneration frequencies [21] than smaller ones ...for Agrobacterium transformation of ...during Agrobacterium co-cul- tivation increased ...
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Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay
... protoplast-based transient transformation assay was recently developed to validate the function of pre- dicted enhancers/promoters in maize ...research. Agrobacterium-mediated transient ...
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The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation
... against Agrobacterium infection; specifically, small interfering RNAs (siRNAs) specific for the T-DNA sequence are generated by the host plant during Agrobacterium infection (Dunoyer et ...of ...
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Application of sonication assisted Agrobacterium mediated transformation in Chenopodium rubrum L
... standard Agrobacterium-mediated transformation procedures remain ...sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient ...
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13. Establishing Agrobacterium mediated transient gene expression assay in Nicotiana tabacum
... be mediated by biolistic gun, microinjection, agrobacterium, electroporation, silicon carbide fiber, liposome, calcium phosphate co- precipitation, polybrene spermidine treatment ...genetic ...
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Optimizing agrobacterium-mediated transformation parameters of melastoma decemfidum ROXB EX JACK
... for Agrobacterium-mediated ...determine transient GUS expression. The transformation efficiency was determined based on the percentage of positive transformed explants as well as the number of ...
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Agrobacterium Mediated Transformation of the Aquarium Plant Cryptocoryne willisii with Gus and gfp Genes
... Several factors are known to influence Agrobacterium- mediated plant transformation. These include bacterial strain and concentration, infection time, co-cultivation period, and pre-culture duration ...
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Optimization of micropropagation and Agrobacterium mediated gene transformation to spinach (Spinacia oleracea L )
... gene transformation was devel- ...Carbeniciline. Transient expres- sion efficiency was calculated based on the number of blue spots per explants one week after ...
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REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF WATERMELON
... An Agrobacterium-mediated transformation system was also established for both ...of transformation experiment, results showed that a number of 20 ...
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Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
... infection, Agrobacterium required to efficiently attached to the host ...of Agrobacterium to plant cell (Matthysse, ...of Agrobacterium to plant cell is affected by many factors including bacterial ...
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High-throughput Agrobacterium-mediated barley transformation
... plant transformation efficiencies have the potential to be as high as 40% if all transgenic callus lines, produced by particle bombardment, regenerated into independently transformed plants ...procedure, ...
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PEG mediated and Agrobacterium mediated transformation in the mycopathogen Verticillium fungicola
... of Agrobacterium-mediated transformants showed integration of transforming DNA as single copies within the target ...of transformation systems for ...
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Agrobacterium Mediated Transformation of Maize (Zea mays L.)
... Abstract Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize ...maize transformation using ...
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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis
... the transformation frequency in liquid IM was 100-fold lower than that on solid IM, the AMT method using liquid IM is better suited for high-throughput insertional mutagenesis because the transformants can be ...
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Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods
... culture-based Agrobacterium-mediated transforma- tion methods such as sonication, at least the risk of somaclonal variation is decreased when seedlings are used as target tissues, the requirement of in ...
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Genetic transformation of wheat via Agrobacterium-mediated DNA delivery
... of transformation, the proce- dure can be conveniently divided into two parts: those steps that ensure effective DNA transfer and integration into the plant nuclear genome and those that allow the regeneration and ...
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Agrobacterium-mediated transformation of Camelina sativa for production of transgenic plants
... Camelina sativa (C. sativa), an oilseed species rich in poly-unsaturated fatty acids, has gained great importance as an industrial oil platform crop in recent years. Despite the potential benefits of C. sativa for ...
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Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum
... Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum Flowers and Vaillancourt, 2005. Current Genetics 48: 380-388 NOTE added by L. ...
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