Alexa Fluor
Inflammatory pre-conditioning restricts the seeded induction of α-synuclein pathology in wild type mice
... e – f , Co-immunofluorescence staining with GFAP (astrocyte marker, Alexa Fluor 594) or NeuN (neuronal marker, Alexa Fluor 594) with 81A antibody (Alexa Fluor 488) shows α Syn inclusions[r] ...
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Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging
... AF488: Alexa Fluor 488; ATCC: American type culture collection; BSA: bovine serum albumin; CDI: N,N'-carbonyldiimidazole; DAPI: 4',6-diamidino-2- phenylindole; DLS: dynamic light scattering; DMEM/ F12: ...
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Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia
... (Euroimmun), Alexa Fluor® (AF) 488 (Invitrogen, Karlsruhe, Germany) or AF568 (Invitrogen), polyclonal donkey anti-human IgG antibodies labeled with Rhodamin Red-X (Dianova, Hamburg, Germany), and poly- ...
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Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth
... Binding of monoclonal antibody to native c-Met expressed on the cell surface of live SNU-5 cells was detected using anti-mouse secondary antibody conjugated with Alexa Fluor®488 dye and [r] ...
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PubMedCentral-PMC4852977.pdf
... of Alexa Fluor 680 conjugated BSA loaded micro-particles are shown in Figure ...with Alexa Fluor 555 conjugated BSA) can then be uniformly dispersed in the reconstitution media composed of ...
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ICAM-1 Binding Rhinoviruses A89 and B14 Uncoat in Different Endosomal Compartments
... Rab11-GTP is required for HRV-A89 infection. Rab11 is in- volved in transferrin transport to the ERC and in recycling to the plasma membrane (32–34). We thus investigated the effect of the constitutively active ...
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The effect of blood coagulation factor XIII on fibrin clot structure and fibrinolysis
... Human fibrinogen, human glu-plasminogen, horse radish peroxidase conjugated (HRP) antibodies to factor XIII-A (SAF-13 HRP) and 2 -antiplasmin (A2AP HRP) were purchased from Enzyme Research Laboratories (Swansea, UK). ...
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Tunable Plasmonic Nanoprobes for Theranostics of Prostate Cancer
... Microscopy images of living cells: bright field (A,C,E,G), time-resolved optical scattering (F,H) and fluorescent (Alexa Fluor 488 conjugated to PSMA antibody, B,D) images of prostate c[r] ...
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PubMedCentral-PMC5225662.pdf
... the Alexa Fluor 647 dye to the N-terminal SNAP-tag of ...of Alexa Fluor 647 to the N-terminal SNAP-tag of Rap1, also bound preferentially to the DNA region containing telomeric repeats (Figure ...
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Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile
... AF: Alexa Fluor; APC: Allophycocyanin; AS: Asherman ’ s syndrome; BSA: Bovine serum albumin; CCR: C-C chemokine receptor; CD: Cycle day; CFU-F: Colony-forming unit-fibroblasts; CM: Central memory; ELISA: ...
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Early Porcine Sapovirus Infection Disrupts Tight Junctions and Uses Occludin as a Coreceptor
... with Alexa Fluor 594-labeled PSaV strain Cowden (400 particles per cell) 48 h after transfection with scrambled, occludin (siOCC), claudin-1 (siCLD-1), JAM-1 (siJAM-1), or ZO-1 (siZO-1) siRNA and incubated ...
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Invasion of Host Cells by JC Virus Identifies a Novel Role for Caveolae in Endosomal Sorting of Noncaveolar Ligands
... (c) Alexa Fluor 594-labeled virus (red) can efficiently enter cells expressing the cav-1 shRNAs, showing that knockdown of cav-1 does not inhibit JCV ...
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Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
... plus Alexa Fluor ® 488 goat anti-mouse IgG as secondary antibody diluted 1:800 (shown in green color) and H + -ATPase (AS07 260) rabbit primary antibody plus Alexa Fluor ® 555 goat anti-rabbit ...
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α Synuclein propagates from mouse brain to grafted dopaminergic neurons and seeds aggregation in cultured human cells
... A 3 hours after addition of 1 μM of Alexa Fluor 488–labeled α-syn proteins in the culture medium, HEK cells expressing α-syn–DsRed have taken up tagged α-syn monomers left, oligomers mid[r] ...
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The Human Polyomavirus, JCV, Uses Serotonin.pdf
... Glial cells were transfected with a GFP- tagged 5HT 2A receptor clone and then incubated with Alexa-fluor 594–labeled JCV at 24 hours after transfection when GFP expression was maximal (26). Virus binding ...
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Development of Norwalk Virus-Specific Monoclonal Antibodies with Therapeutic Potential for the Treatment of Norwalk Virus Gastroenteritis
... of Alexa Fluor 488, which is directed to the c-myc tag at the C terminus of VP1 (display fluorescence), and DyLight 649, which is directed to the MAb/Fabs (Ab binding ...
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Increased frequency of circulating IL 21 producing Th cells in patients with granulomatosis with polyangiitis (GPA)
... Abbreviations ANCA: anti-neutrophil cytoplasmic autoantibodies; A488: Alexa Fluor®® 488; APC: allophycocyanin; BAFF: B-cell activating factor; BVAS: Birmingham Vasculitis Activity Score;[r] ...
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Endosomal Localization of the Serum Resistance-Associated Protein in African Trypanosomes Confers Human Infectivity
... The initial interaction between TLF-1 and SRA does not occur within the flagellar pocket. Previous studies demon- strated that SRA binds TLF-1 via apoL-1 (39) and that these proteins colocalize in intracellular vesicles ...
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The absence of retinal input disrupts the development of cholinergic brainstem projections in the mouse dorsal lateral geniculate nucleus
... To collect brain tissue for analysis, mice were deeply anaesthetized by hypothermia (<P5) or isoflurane vapors, and transcardially perfused with phosphate-buffered sa- line (PBS, 0.01 M phosphate buffer with 0.9% ...
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Inhibition of HMGB1/RAGE-mediated endocytosis by HMGB1 antagonist box A, anti-HMGB1 antibodies, and cholinergic agonists suppresses inflammation
... with Alexa Fluor was per- formed according to the manufacturer’s instructions (Molecular ...with Alexa Fluor-labeled HMGB1, LPS or LPS plus HMGB1 in the presence or absence of increasing ...
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