Allele specific PCR
An improved allele-specific PCR primer design method for SNP marker analysis and its application
... (Allele-specific PCR) are widely used for low-throughput applications in plant re- ...using allele-specific PCR primers based on the 3’ terminal nucleotide of a primer that ...
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Rapid determination of clonality by detection of two closely linked X chromosome exonic polymorphisms using allele specific PCR
... sensitive, specific, reproducible, quantitative, and applicable to nonnucleated ...of allele-specific oligonucle- otide hybridization (ASOH) (13), PCR-ASOH (14), and PCR amplification ...
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Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
... heterozygous allele can allow adequate amplification of both alleles thus avoiding ...fied PCR program on ADO ...more allele-specific primer for GG genotype to bind into its sequence target ...
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Allele specific PCR detection of sweet cherry self incompatibility alleles S3, S4 and S9 using consensus and allele specific primers in the Czech Republic
... KCl PCR buffer without MgCl 2 , ...the PCR prim- ers, ...The PCR amplification consisted of following steps: initial denaturation at 95°C for 2 min followed by 36 cycles consisting of denaturation at ...
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Detection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
... and PCR restriction fragment polymor- phism (PCR-RFLP) have been extensively used for detection of EMB resistance- related ...mutiplex allele-specific (MAS)-PCR has received a great ...
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454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine <em>KRAS</em> mutation analysis of formalin-fixed, paraffin-embedded samples
... real-time PCR, pyrosequencing, and chip array hybridization, ...real-time PCR, pyrosequencing, and chip array hybridization showed higher sensitivity but suffered from the limitations of predesigned ...
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Direct Detection of Erythromycin Resistant Bordetella pertussis in Clinical Specimens by PCR
... simple PCR method for detection of erythromycin-resistant ...is specific, rapid, and easily un- dertaken compared to currently available ...by allele-specific PCR assays 1 and ...
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PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
... The allele-specific PCR technique was applied as described by ...two allele-specific reverse primers, where the 3′ prime end is specific for a SNP ...the ...
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Development of a highly sensitive method for detection of JAK2V617F
... on specific PCR primers to discriminate wild type and mutant ...mutant allele for detection of JAK2V617F ...is PCR-restriction fragment length polymorphism, which takes advantage of the fact ...
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A Nested Allele-Specific Multiplex Polymerase Chain Reaction Method for the Detection of DRD2 Polymorphisms
... of PCR products for sizing, mutations, or ...more specific when compared with agarose gel electrophoresis ...the PCR-agarose gel electrophoresis method and the PCR-microchip electrophoresis ...
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Enzymatic and molecular characterization of insecticide resistance mechanisms in field populations of Aedes aegypti from Selangor, Malaysia
... for allele‑specific PCR (AS‑PCR) genotyping of the gene to detect the presence of F1534C, V1016G and S989P ...(double allele) and F1534C/V1016G/S989P (triple allele) mutations ...
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DETECTION AND DIFFERENTIATION OF HUMAN PROTOZOAN PARASITES IN STOOL SPECIMENS BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City, Egypt
... Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) assay and microscopy for detection and identification of common pathogenic protozoan parasites in New Damietta city of North Delta ...
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DETECTION OF HUMAN INTESTINAL PROTOZOA BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City
... Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) assay and microscopy for detection and identification of common pathogenic protozoan parasites in New Damietta city of North Delta ...
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Association of CYP2B6 Genotype with Survival and Progression Free Survival in Cyclophosphamide Treated Multiple Myeloma
... used allele specific PCR and Pyrosequencing to investigate the impact of CYP2B6 SNPs G516T, A785G and C1459T, GSTP1 Ile105Val, and GSTM1 and GSTT1 variants, on the outcome for 26 cyclophosphamide ...
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Characterization of single nucleotide polymorphism in the 5'-untranslated region (5'-UTR) of Lactoferrin gene and its association with reproductive parameters and uterine infection in dairy cattle
... using Allele-specific PCR method in cows with and without uterine ...of Allele specific PCR was in complete agreement with sequencing ...
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The involvement of Kras gene 3'-UTR polymorphisms in risk of cancer and influence on patient response to anti-EGFR therapy in metastatic colorectal cancer: a meta-analysis
... AS-PCR, allele-specific PCR; PCR, polymerase chain reaction; PCR-RFLP; PCR–restriction fragment length polymorphism; BEL, Belgium Ovarian Cancer study; BWh, Brigham ...
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PCR based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato
... The Leu-Phe mutation at the kdr locus in An. culicifacies s.l. was reported by Hoti et al [11] using an allele-specific PCR assay, whose external primers Agd1 and Agd2 were based on An. gambiae ...
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Allele specific real time PCR testing for minor macrolide resistant Mycoplasma Pneumoniae
... of PCR products, real-time PCR, pyrosequencing, restriction fragment length polymorphism (RFLP) analysis, high-resolution melting curve analysis and allele-specific PCR ...of PCR ...
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EST SNPs in bread wheat: discovery, validation, genotyping and haplotype structure
... genome specific primers were designed from the sequences of two genes involved in starch biosynthesis (GSS and Xwye838) gave further support to the desirability/feasibility of the above NSF funded ...
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Blood pressure–associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding
... AllPrep DNA/RNA Kit (Qiagen). Deletion efficacy was tested by PCR using primers that flanked the deleted region. ARHGAP42 message was measured using semiquantitative PCR as described above. Guide RNAs used ...
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