ALVEOLAR TYPE 2 CELLS
Type 2 alveolar cells are stem cells in adult lung
... of type 2 and type 1 epithelial cells (AEC2s and AEC1s), capillaries, and various resident mesenchymal ...to alveolar maintenance and ...long-term alveolar stem ...and ...
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Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells
... 264.7 cells were swollen and lacked cristae exhibiting apoptotic cell ...BEAS2-B cells, the mitochondria were condensed and eventually disappeared exhibiting necrotic cell ...(Figs. 2, 4, 5), an ...
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The development and plasticity of alveolar type 1 cells
... AT1 cells fold in conjunction with alveolar ...AT2 cells (asterisk) by Hopx CreER when induced with 2 mg tamoxifen at ...AT1 cells; open arrowhead, AT2 ...AT1 cells; Asterisk, ...
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Proliferative regulation of alveolar epithelial type 2 progenitor cells by human Scnn1d gene
... numerous cells and tissues [4, 8], and regulates the biophysical features and proteolytic properties of αβγ channels [13-15], the function of δ-ENaC remains ...epithelial cells, and ~ 40% of ...
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Overexpression of cathepsin K in mice decreases collagen deposition and lung resistance in response to bleomycin-induced pulmonary fibrosis
... < 2 yr from time of diagnosis ...by alveolar epithelial cell injury and hyperplasia, inflammatory cell accumulation, fibrob- last hyperplasia, formation of honeycomb cysts and exu- berant production and ...
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Hermansky-Pudlak syndrome type 2 manifests with fibrosing lung disease early in childhood
... hyperplastic type II pneumocytes (G: HE, x400) as subtle but characteristic features of HPS ...in alveolar epithelial cells type II (AECII) in HPS2 patient ...
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Alert cell strategy in SIRS-induced vasculitis: sepsis and endothelial cells
... epithelial cells and vascular endothelial ...dendritic cells, and the overproduction of inflammatory ...epithelial cells in major organs express inflammatory receptors on their surface and can ...
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Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells.
... Lung tissues were fixed in formalin overnight, then dehydrated, cleared, and embedded as follows: 70% ethanol for 3 h, 80% ethanol for 3 h, 95% ethanol for 2 h, 100% ethanol for 1.5 h (twice), xylene for 0.5 h ...
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Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
... (Figs. 2, 3, 4). Increased immunoreactive WIF1 (Fig. 2) and FST ...the alveolar epi- thelial type I and II cells, bronchial epithelial cells, and macrophages of JF1/MsJ compared ...
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Mechanisms of lung fibrosis induced by carbon nanotubes: towards an Adverse Outcome Pathway (AOP)
... adenocarcinoma alveolar epithelial cell line; ATII: primary human alveolar type-II epithelial cells; BALB-3 T3: mouse embryonic fibroblast cell line; BEAS-2B: human bronchial epithelial cell ...
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Wnt signaling regulates trans-differentiation of stem cell like type 2 alveolar epithelial cells to type 1 epithelial cells
... for alveolar morphogenesis it is not essential for the development of proximal airways ...alter alveolar epithelial differentiation [18] and can give rise to modified molecular microenvironments that ...
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Nuclear factor-kappa B influences early phase of compensatory lung growth after pneumonectomy in mice
... in type 2 alveolar epithelial cells and was significant increased 12 h after pneumonectomy compared to 48 h after pneumonectomy and both 12 and 48 h after thoracotomy ( p < ...
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Induced pluripotent stem cell-derived lung alveolar epithelial type II cells reduce damage in bleomycin-induced lung fibrosis
... 400,000 cells/well in 12 MW in Essential 8™ Flex Medium with Rock ...the cells were replated on human placenta collagen IV (Sigma)-coated 6 MW at about 200,000 cells/cm 2 in basal ...
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LysoTracker is a marker of differentiated alveolar type II cells
... mins. Cells were washed and resuspended in a cocktail of fluorescein-conjugated antibodies including rat anti-mouse CD45, CD31 and EpCAM (Biolegend) and incubated on ice for 20 ...Labeled cells were washed ...
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Human alveolar epithelial cells type II are capable of TGFβ-dependent epithelial-mesenchymal-transition and collagen-synthesis
... these cells and seem to interfere with a TGFβ-mediated effect in ...the Type 2 EMT of ...primary cells might acquire the Type 2 EMT state only transiently and reverse their ...
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Culture of human alveolar epithelial type II cells by sprouting
... epithelial cells from two different donors were used ...The cells were lysed with Pierce IP lysis buffer (Thermo Fischer Scientific, Waltham, MA, USA) according to the manufacturer’s ...
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Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells
... Figure 2: The strong, dose- and time-dependent rise in fluores- cence is due to the abundance of intracellular lipid/water- interfaces, into which FM 1-43 can intercalate, and an increase in drug-induced membrane ...
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Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
... that of its mouse homolog. As shown in Fig. 5e, miR-371b- 5p and its mouse homologous miR-292b-5p do share ap- proximately 91% sequence homology. For clarification, we designed two reverse primers, R-primer-I and ...
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Fraction of MHCII and EpCAM expression characterizes distal lung epithelial cells for alveolar type 2 cell isolation
... Backgound: Alveolar type 2 (AT2) cells play important roles in maintaining adult lung ...AT2 cells isolated from the lung have revealed the cell-specific functions of AT2 ...AT2 ...
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Inflammatory and cytotoxic responses of an alveolar-capillary coculture model to silica nanoparticles: Comparison with conventional monocultures
... m 2 ) [8], which makes it an interesting target for drug and gene delivery, but at the same time represents a serious point of attack for harm- ful ...this alveolar-capil- lary barrier is difficult to ...
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