Amniotic epithelial cells
Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity
... thelial cells [2–4]. These human amniotic epithelial cells (hAECs) have unique properties that distinguish them from other human cell types including immunomodu- latory effects and stem ...
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Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure
... human amniotic epithelial cells (hAECs) that can differentiate into cell types that arise from all three germ layers may offer promise for restoration of such ...into cells that express germ ...
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Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse model
... human amniotic epithelial cells (hAECs) have stem cell characteristics; however, it is unclear whether hAECs have the therapeutic potential to restore fertility after ...stem cells (hEnSCs) ...
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Molecular characterization and in vitro differentiation of feline progenitor-like amniotic epithelial cells
... Isolation of amniotic epithelial cells and cell culture Amnion fragments were washed twice in Hank’s Balanced Salt Solution (Euroclone, Milano, Italy. ECB-4007 L) sup- plemented with penicillin 100 ...
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Original Article Amniotic epithelial cells reverse abnormal vascular structure and function in endometrial carcinoma
... [2-4]. Amniotic epithelial cells (AECs) are used in tissue engineering to increase blood perfu- sion and promote wound healing ...of amniotic epithelial stem cells in “tumor ...
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Original Article Pathologic changes of wound tissue in rats with stage III pressure ulcers treated by transplantation of human amniotic epithelial cells
... Abstract: This study aims to determine the impact of orthotopic transplantation of human amniotic epithelial cells (hAECs) on the pathologic changes of wound tissues in a self-prepared rat stage III ...
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Identification of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible genes in human amniotic epithelial cells
... The amniotic membrane is of fetal origin but contains no blood vessels ...The amniotic epithelial cells (AEC) on the basement membrane which is the thickest among basement membranes in human ...
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Original Article Functional regeneration of irradiated salivary glands with human amniotic epithelial cells transplantation
... human amniotic epithelial cells (hAECs) transplantation by intra-glandular ...these cells were positive for CD29, CD73 and CK19 and negative for CD44, CD34, CD45 and ...acinar-like ...
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Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence
... Amniotic epithelial cells (AECs) are a class of fetal stem cells that derives from the epiblast and resides in the amnion until ...fibroblast cells with conditioned medium from rat ...
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Vitamin C improves the therapeutic potential of human amniotic epithelial cells in premature ovarian insufficiency disease
... stem cells have been in- vestigated to treat POI, including adipose-derived mesenchymal stem cells (hADSCs) [6], fetal liver mes- enchymal stem cells [9], bone marrow mesenchymal stem cells ...
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The effect of lipopolysaccharide on the expression level of immunomodulatory and immunostimulatory factors of human amniotic epithelial cells
... these cells are proposed as a novel source of stem cells that differ- entiate into different cell types originating from three germ layers without any of the ethical concerns related to human stem ...
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Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells
... reactions but also zoonosis. Over the last decade alterna- tive cryoprotective substances, which avoid these potential problems have been developed, including disaccharide tre- halose [33], oligosaccharide [34], and ...
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Chorioamnionitis (ChA) modifies CX3CL1 (fractalkine) production by human amniotic epithelial cells (HAEC) under normoxic and hypoxic conditions
... Isolated cells had a polygonal epithelioid appearance with perinuclear inclusions and were almost homogenous (mini- mum 97% of ...of cells were posi- tive for specific simple epithelial cytokeratin ...
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Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli
... This process is comprised of binding to the extracellular matrix (ECM) ligands and activating the specific signaling pathways which would be involved in the mechanical- induced differentiation of cells [31]. ...
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Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage
... stem cells transplant- ation alter the local microenvironment in injured tissue by secreting a broad range of trophic and immunomod- ulatory factors that can be harvested in serum-free conditioned medium (CM), ...
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Human amniotic epithelial cells inhibit granulosa cell apoptosis induced by chemotherapy and restore the fertility
... stem cells de- rived from a variety of human tissues including skin- derived mesenchymal stem cells [5], adipose-derived stem cells [6] and human amniotic fluid stem cells [7, 8] could ...
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Tissue-specific human beta-defensins (HBD)-1, HBD-2 and HBD-3 secretion profile from human amniochorionic membranes stimulated with Candida albicans in a two-compartment tissue culture system
... Human amniotic epithelial cells; HNP: Human neutrophil peptide; LPS: Lipopolysacharide; MIAC: Microbial invasion of the amniotic cavity; PROM: Premature rupture of membranes; SLPI: Secretory ...
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Protocol for the Ovine Amniotic Epithelial Cell In Vitro Culture and Differentiation
... stem cells, easy to obtain without any ethical concerns [1-3]. Ovine Amniotic epithelial cells (oAEC) are a subset of fetal stem cells located into the inner layer of the ...
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Therapeutic Strategies of Stem Cell Transplantation for Liver Cirrhosis
... stem cells and the need for pre-differentiation into he- patocyte-like cells prior to transplantation, and providing an overview of the methods to improve cell viability and to prevent the exacerbation of ...
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Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane
... sufficient cells for cell-based therapy of skin ...iPS cells at the early stage of epithelial differen- tiation by precisely regulating the EGF signaling pathway, and then obtained multipotent ...
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