Amphipathic Helix
Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
... first test of this hypothesis, we used PCR mutagenesis (14) to construct a mutant of pBRTM/HCV 827–3011, termed 827/ 5A⌬7–28, wherein the predicted AH of NS5A was deleted. This mutation resulted in a dramatic change in ...
7
An N-Terminal Amphipathic Helix in Hepatitis C Virus (HCV) NS4B Mediates Membrane Association, Correct Localization of Replication Complex Proteins, and HCV RNA Replication
... N-terminal amphipathic helix (AH) in NS4B that mediates membrane ...the amphipathic nature of NS4B’s N-terminal helix abolished HCV RNA replication, whereas partial disruption resulted in an ...
8
The Amphipathic Helix of Influenza A Virus M2 Protein Is Required for Filamentous Bud Formation and Scission of Filamentous and Spherical Particles
... membrane-proximal amphipathic helix that facilitates the scission process and is also required for filamentous particle ...the amphipathic helix (M2 five-point mutant, or 5PM) reduced scission ...
10
A C terminal amphipathic helix is necessary for the in vivo tubule shaping function of plant reticulon
... Recently, Brady et al. (11) used solution NMR of lipid- and detergent-containing micelles of recombinant Yop1p to gain structural insights in this class of ER tubule-shaping proteins. They identified a novel structural ...
31
The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid binding amphipathic helix
... C-term amphipathic helix and once diffused to the LD surface, it forms cis CIDEA complexes by interacting through the C-term (126–155) ...The amphipathic helices, embedded in the hydrophobic region ...
25
Apolipoprotein A I and its amphipathic helix peptide analogues inhibit human immunodeficiency virus induced syncytium formation
... into amphipathic alpha-helixes, and these regions have been suggested to play a role in subunit associations and in virus- induced cell fusion and cytopathic effects of ...that amphipathic ...
10
The Amphipathic Helix of Adenovirus Capsid Protein VI Contributes to Penton Release and Postentry Sorting
... phipathic helix capable of membrane association and lysis in vitro and in vivo (18, 22, ...the helix is shielded through association with hexons (12, ...the amphipathic helix performed in the ...
15
Evidence that Insertion of Tomato Ringspot Nepovirus NTB-VPg Protein in Endoplasmic Reticulum Membranes Is Directed by Two Domains: a C-Terminal Transmembrane Helix and an N-Terminal Amphipathic Helix
... hours after transfection, the red fluorescence associated with ER-dsRed2 was clearly distinguished from the duller chloro- plast autofluorescence (i.e., cells adjacent to those expressing the ER marker were not visible ...
14
A Conserved Amphipathic Helix in the N-Terminal Regulatory Region of the Papillomavirus E1 Helicase Is Required for Efficient Viral DNA Replication
... Mutation of the E1 amphipathic helix affects DNA replica- tion at a step following assembly of the E1-E2-ori complex. In principle, the E1 MFI mutation could lower viral DNA repli- cation by reducing the ...
14
Coxsackie B3 virus protein 2B contains cationic amphipathic helix that is required for viral RNA replication.
... pathic helix motifs with high helical hydrophobic moments ...well-conserved amphipathic helix motif in enterovirus protein 2B with a narrow hydrophilic face and an arrangement of cationic residues ...
11
Unique Mutational Patterns in the Envelope α2 Amphipathic Helix and Acquisition of Length in gp120 Hypervariable Domains Are Associated with Resistance to Autologous Neutralization of Subtype C Human Immunodeficiency Virus Type 1
... 18-residue amphipathic helix ( ␣ 2) located on the gp120 outer ...2 helix between resistant donor Envs and sensitive recipient Envs did not alter the NAb ...2 helix must be linked to still ...
11
A Point Mutation in the N-Terminal Amphipathic Helix α0 in NS3 Promotes Hepatitis C Virus Assembly by Altering Core Localization to the Endoplasmic Reticulum and Facilitating Virus Budding
... Our study identified, for the first time to our knowledge, an adaptive mutation within the N-terminal helix ␣0 domain of NS3 that significantly enhanced virus assembly while having no effec[r] ...
13
An N-Terminal Amphipathic Helix in Dengue Virus Nonstructural Protein 4A Mediates Oligomerization and Is Essential for Replication
... NS4A, a transmembrane endoplasmic reticulum (ER) resident protein, is thought to induce the host membrane modifications that harbor the viral RC (3). A similar function for NS4A was reported in other flaviviruses (4, 5). ...
6
Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication
... N-terminal amphipathic helix in protein 2C was replaced with the corresponding region from HRV14 produced very small plaques on HeLa cell monolayers and displayed delayed kinetics and low yields of RNA in a ...
11
MBAR enhanced lattice Monte Carlo simulation of the effect of helices on membrane protein aggregation
... an amphipathic helix (APH) that lies along the membrane surface. The two he- lices form approximately a right angle and are connected by a small loop. The TatA structure of bacterium Bacillus subtilis has ...
34
Amphipathic α-Helix AH2 Is a Major Determinant for the Oligomerization of Hepatitis C Virus Nonstructural Protein 4B
... resolved amphipathic ␣ -helix (AH1 and AH2, re- spectively), a central part harboring four predicted transmem- brane segments, and a C-terminal part comprising a predicted and a structurally resolved ␣ ...
9
Sequence analysis reveals a conserved extension in the capping enzyme of the alphavirus supergroup, and a homologous domain in nodaviruses
... Results: We examined over 50 genera of viruses by sequence analyses . Earlier studies showed that the MTase-GTase contains a “ Core ” region conserved in sequence. We show that it is followed by a long extension, which ...
21
Identification of a Novel Determinant for Membrane Association in Hepatitis C Virus Nonstructural Protein 4B
... an amphipathic ␣ -helix predicted within NS4B aa 1 to 26 mediates membrane associ- ation ...with amphipathic properties (data not ...first amphipathic helix may participate in the dy- ...
12
Molecular Determinants for Subcellular Localization of the Severe Acute Respiratory Syndrome Coronavirus Open Reading Frame 3b Protein
... an amphipathic ␣ -helix often found in the C terminus of these proteins (1, 7, 15, ...(H, helix) made by ...predicted amphipathic helix formed by amino acids 74 to ...
10
CHARACTERIZATION OF 9-CIS-EPOXYCAROTENOID DIOXYGENASE3 GENE FROM Hevea brasiliensis AND ITS EXPRESSION RESPONSES BY TISSUE TYPE DURING DROUGHT STRESS
... similar to each other, respectively. The putative a-amphipathic helix is underlined. A phylogenetic tree constructed using NCED genes of 18 plant species demonstrated Hb- NCED3 was c[r] ...
8