Amplified Fragment Length Polymorphism
Use of Fluorescent Amplified Fragment Length Polymorphism for Molecular Epidemiology of Leptospirosis in India
... Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using ...
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Genomic Diversity of Clinical and Environmental Vibrio cholerae Strains Isolated in Brazil between 1991 and 2001 as Revealed by Fluorescent Amplified Fragment Length Polymorphism Analysis
... Vibrio cholerae is a ubiquitous and abundant organism in aquatic environments, particularly in coastal areas, estuaries, and rivers. This organism was the cause of a considerable number of deaths in Brazil during the ...
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Fluorescent Amplified Fragment Length Polymorphism Genotyping of Campylobacter jejuni and Campylobacter coli Strains and Its Relationship with Host Specificity, Serotyping, and Phage Typing
... Fluorescent amplified fragment length polymorphism (FAFLP) analysis was applied to 276 Campylobacter jejuni strains and 87 Campylobacter coli strains isolated from humans, pigs, cattle, ...
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Experimental versus In Silico Fluorescent Amplified Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis: Improved Typing with an Extended Fragment Range
... fluorescent amplified fragment length polymorphism (FAFLP) data were compared with in silico data for the sequenced strains of Mycobacterium tuberculosis (H37Rv and ...identify ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... Telenti et al. (32). PRPA differs from the latter technique in both the targeted region for PCR (i.e., the 3⬘ end of the 16S rRNA gene) and the use of three restriction enzymes (RsaI, DraI, and EcoNI). As a follow-up to ...
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Genetic Diversity of Actinobacillus pleuropneumoniae Assessed by Amplified Fragment Length Polymorphism Analysis
... Amplified fragment length polymorphism (AFLP) was evaluated as a method for genotypic characterization and subtyping within the bacterial species Actinobacillus ...
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A Predominant and Virulent Legionella pneumophila Serogroup 1 Strain Detected in Isolates from Patients and Water in Queensland, Australia, by an Amplified Fragment Length Polymorphism Protocol and Virulence Gene Based PCR Assays
... standardized amplified fragment length polymorphism (AFLP) protocol of the European Working Group on Legionella Infection to subtype ...
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Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR Amplified Fragment Length Polymorphism Analysis of Methicillin Resistant Staphylococcus aureus
... cent amplified fragment length polymorphism (5), binary typ- ing (23), variable number of tandem repeat analysis (14), and others (1, 2, ...multienzyme amplified fragment ...
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Identification of Clostridium Species and DNA Fingerprinting of Clostridium perfringens by Amplified Fragment Length Polymorphism Analysis
... An amplified fragment length polymorphism (AFLP) method was applied to 129 strains representing 24 different Clostridium species, with special emphasis on pathogenic clostridia of medical or ...
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Most Cases of Cryptococcal Meningitis in HIV Uninfected Patients in Vietnam Are Due to a Distinct Amplified Fragment Length Polymorphism Defined Cluster of Cryptococcus neoformans var grubii VN1
... clear. Amplified fragment length polymorphism (AFLP) analysis was used to characterize the relationship between strains infecting HIV-infected and uninfected ...
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Genome Sequence Based Fluorescent Amplified Fragment Length Polymorphism Analysis ofMycobacterium tuberculosis
... the polymorphism associated with insertion sites of a single mobile ...three fragment differences) might be assigned to the same strain if the epidemiological context so indicated, ...
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Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... 40), amplified fragment length polymorphism (5, 15), flagella gene PCR-restriction fragment length polymorphism (PCR-RFLP) (1, 4, 23, 24, 26), ribotyping (6, 25, 36), and ...
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Use of an Amplified Fragment Length Polymorphism Technique To Fingerprint and Differentiate Isolates of Helicobacter pylori
... FIG. 1. Diagram illustrating the AFLP technique used in the study. (A) The double-stranded fragment produced from digestion with HindIII (recognition site A2AGCTT). (B) Sequences of the two complementary ...
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Genotyping of Epidemic Methicillin ResistantStaphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified Fragment Length Polymorphism Analysis
... Fluorescent amplified-fragment length polymorphism (FAFLP) analysis was investigated for its ability to identify and subtype isolates of an epidemic methicillin-resistant phage type of ...
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Fluorescent Amplified Fragment Length Polymorphism Analysis of an Outbreak of Group A Streptococcal Invasive Disease
... Fluorescent amplified-fragment length polymorphism (FAFLP) analysis was carried out for an outbreak of group A streptococcal (GAS) invasive ...dye. Amplified fragments of up to 600 bp ...
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Epidemiology of Human Sporotrichosis Investigated by Amplified Fragment Length Polymorphism
... Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of Peruvian strains of Sporothrix schenckii and to compare them to a panel of non-Peruvian ...
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Molecular Study of the Genetic Variability of Pumpkins Landraces from Brazilian Amazon
... The Cucurbita maxima Duchesne is a vegetable crop plant cultivated and maintained by traditional Amazon communities, Brazil. The situation is wor- sened by the possibility of disappearance of local populations and ...
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Genotyping Neisseria gonorrhoeaeUsing Fluorescent Amplified Fragment Length Polymorphism Analysis
... sized fragment patterns that can be stored in a database, and it is of particular value for objectively genotyping large numbers of ...the fragment at 201 bp (boxed) is present for strains 13072 and 16973 ...
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Dissemination of Two Methicillin Resistant Staphylococcus aureus Clones Exhibiting Negative Staphylase Reactions in Intensive Care Units
... restriction fragment length polymorphism profiles of the staphylococcal coagulase gene, and random amplified polymor- phic DNA patterns generated by arbitrarily primed PCR of the isolates ...
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Amplified Fragment Length Polymorphism Fingerprinting of Mycoplasma Species
... As revealed by cluster analysis, AFLP fingerprints of the analyzed mycoplasmas retain attributes of species specificity, leading to the conclusion that the method may be used as an additional tool for species ...
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