and HEPES
Short Communication: Fish Hepatocytes Accumulate Hepes: A Potential Source of Error in Studies of Intracellular pH Regulation
... Assuming that a mean of these values 67% is approximately correct for toadfish hepatocytes, then l.OSmmolP 1 Hepes accumulation at lOmmolP 1 external Hepes concentration would increase t[r] ...
6
Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer
... radiolabelling: HEPES and MES, and com- pared this with the most commonly used acetate buffers: sodium acetate and ammonium acetate, and showed that an increased SA could be obtained when DTPA- and DOTA-conjugated ...
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Modulation of the inflammatory response by taurine and its structural analogue, HEPES
... or HEPES to form taurinechloramine and HEPESchloram ine respectively (Lambert et ...and HEPES is associated with increased M PO activity most likely reflecting increased activity through the M P 0 -H 2 0 2 ...
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HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
... Consistently, HEPES-dependent MiT/TFE nuclear redistri- bution was independent of changes in MTORC1 activity, as judged by the phosphorylation status of its downstream targets RPS6 and ...by HEPES appears ...
14
Development and evaluation of a rapid analysis for HEPES determination in 68Ga-radiotracers
... of HEPES content in radiopharmaceutical formulations were ...the HEPES content in radio- pharmaceutical product formulations in our laboratory ...facilitates HEPES determination substantially and is ...
10
Identification of novel target genes in human lung tissue involved in chronic obstructive pulmonary disease
... The tissue pieces were rinsed with cell buffer (pH 6.64, 10 mM HEPES, 1.9 mM CaCl 2 , 1.3 mM MgSO 4 , 137 mM NaCl, 5.4 mM KCl, 6.2 mM glucose, 6.2 mM Na 2 HPO 4 ) and incubated for 60 min at 400 rpm, at 37 ° C in ...
5
3 (Morpholinium 1 yl)propanesulfonate
... and HEPES {[4-(2-hydroxyethyl)-1-piperazine]ethanesulfonic acid}, has allowed better study of many biological processes (Good et ...and HEPES (Wouters et ...
9
Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: A case study with the metastatic breast cancer cell line MDA-MB-231
... There have been few rigorous studies demonstrating e ff ective washing and quenching methods for adherent cell cultures. The use of buffer additives such as AMBIC, HEPES and NaCl have been evaluated with ...
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Adsorptive Stripping Voltammetric Determination of Cobalt in the Presence of Nickel and Zinc Using Pyrogallol Red as Chelating Agent
... Water (10.0 mL), 1.0 mL of Britton–Robinson buffer (0.4 mol L –1 ) or 1.0 mL of HEPES buffer (0.5 mol L –1 ), 20 μL of pyrogallol red (1x10 –3 mol L –1 ), and aliquots of Co(II) solution (0.5 mg L –1 ) were ...
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FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth
... and eosin; HEPES: 4-2-hydroxyethyl-1-piperazineethanesulfonic acid; HER2: human epidermal growth factor receptor 2; IC50: 50% inhibitory concentration; IDC: invasive ductal carcinoma; Ig[r] ...
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Integrated electrodes on a silicon based ion channel measurement platform
... Single channels of OmpF porin in 0.50 M KCl, 20 mM HEPES, at pH 7.4 measured using the integrated electrodes on the PTFE coated, SU-8 patterned, silicon device at a holding potential of [r] ...
8
Low Molecular Weight Heparin-Coated and Dendrimer-Based Core-Shell Nanoplatform with Enhanced Immune Activation and Multiple Anti-Metastatic Effects for Melanoma Treatment
... Whether LMWH/PPD/CpG nanoparticles could inhibit and eradicate B16F10 pulmonary metastasis was another central issue. We assumed that LMWH/PPD/CpG exhibited the anti-metastatic effects by integrating the anti-metastatic ...
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Fluorosugars inhibit biological properties of different enveloped viruses.
... After three successive washes with prewarmed phosphate-buffered saline, the cells were flooded with 100 ul of pyruvate containing Earle medium buffered with HEPES N-2-hydroxymethyl-piper[r] ...
5
Threshold Extension of Gallium Arsenide/Aluminum Gallium Arsenide Terahertz Detectors and Switching in Heterostructures
... Figure 6.2 SPR sensorgrams for binding of CX 2406 to the immobilized G-quadruplex formed by c-MYC top, human telomere middle and duplex DNA bottom as a hairpin dimer in HEPES buffer cont[r] ...
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Effect of buffer at nanoscale molecular recognition interfaces - electrostatic binding of biological polyanions
... (or HEPES), presumably because competitive interactions of the latter buffers with the cationic micelle limit the enthalpic gain. Further, S increases from Tris-HCl to PBS, suggesting greater displacement of ...
6
Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut
... d Wall-eyed stereo view of the wider active site with the additional fortuitous HEPES molecule white carbon atoms shown revealing the likely role of the PA14 domain purple in extending [r] ...
15
Original Article Homocysteine induces cardiac hypertrophy by up-regulating ATP7a expression
... in Hepes-buffered Dulbecco’s modification of Eagle’s medium supplemented with 10% fetal bovine serum at 37°C in the presence of 5% CO 2 and transfect- ed with 30 nM siRNAs that could target-silence ATP7a, using ...
8
Radiolabeling polymeric micelles for in vivo evaluation: a novel, fast, and facile method
... Prior to the optimization of the radiolabeling parame- ters, the distribution ratio, D , of 111 In in a mixture of chloroform and HEPES buffer containing tropolone had been determined.. [r] ...
10
Testing Ostrom: an Analysis of Water User Committees in Uganda
... In conclusion, there are many ways to improve these chemosensors by optimization of existing methods and new rational design. The linker TESPSA chemistry requires more experiment to determine whether it could be more ...
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Analysis of Oocyte Quality in the Rhesus Macaque (Macaca mulatta)
... Method A (Si et al., 2000) was developed for rhesus monkey spermatozoa. Freezing medium was prepared according to published protocol for “TTE” solution (Sankai et al., 1994), including use of fresh egg yolk and the ...
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