and human hepatocytes
Loss of nuclear PTEN in HCV-infected human hepatocytes
... in human hepatocytes (transfected with HCV genomic RNA) at 3, 6 and 12 days post-transfection (Figure 3A, B) and repeated the analyses in cells trans- fected with increasing amounts of HCV genomic RNA ...
11
Detection of nanolevel drug metabolites in an organotypic culture of primary human hepatocytes and porcine hepatocytes with special reference to a two-compartment model
... between human and pig ...in human hepatocytes in both the supernatant and in the matrix at 24 hours of exposure time, which is not reduced in comparison to porcine ...porcine hepatocytes was ...
14
Induction of Cytochrome P450 2A6 by Bilirubin in Human Hepatocytes
... tion between total bilirubin level and systemic clearance of letrozole was unexpected. The elimination pathway of letrozole is metabolism to carbinol-metabolite CGP44645 by hepatic CYP2A6 and CYP3A4, followed by its glu- ...
9
Effect of honokiol on the induction of drug-metabolizing enzymes in human hepatocytes
... Abstract: Honokiol, 2-(4-hydroxy-3-prop-2-enyl-phenyl)-4-prop-2-enyl-phenol, an active component of Magnolia officinalis and Magnolia grandiflora, exerts various pharmacological activities such as antitumorigenic, ...
9
Generation of functional human hepatocytes in vitro: current status and future prospects
... uPA/SCID mice have some disadvantages. Repopulation of human hepatocytes in the liver of these mice is de- creased due to deletion of the uPA transgene by hom- ologous recombination. In addition, ...
9
The metabolomics of acetaminophen toxicity observed in human biofluids and cultured primary human hepatocytes
... rat hepatocytes could allow for the examination of hepatocyte response to the toxic effects of hepatocytes in the fasted or fed state, instead of the apparent stressed-fasted state of the human ...
161
Original Article Hedgehog signaling pathway regulates liver regeneration in the Fah knockout mice model xenografted by human hepatocytes
... week old males) that were injected i.p. with vehicle (olive oil) or cyclopamine (15 mg/kg/ day) 24 h before human hepatocytes transplan- tation and daily thereafter. Liver remnants and blood were harvested ...
9
Differential kinetic profiles and metabolism of primaquine enantiomers by human hepatocytes
... The enantiomers of PQ showed significant variation in in vitro pharmacokinetics as well as metabolite gen- eration in human hepatocytes. The in vitro clearance of (−)-PQ is three times faster than that of ...
12
Alpha Interferon Inhibits Hepatitis C Virus Replication in Primary Human Hepatocytes Infected In Vitro
... healthy human hepatocytes at the concentrations used in this ...of hepatocytes from three different donors (FT154, FT155, and FT156), treated for 5 days with 5,000 U of IFN-␣ per ml, revealed no ...
11
Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes
... Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core protein-mediated immor- talized ...
7
Vectorial Entry and Release of Hepatitis A Virus in Polarized Human Hepatocytes
... polarized hepatocytes is therefore not surprising and readily accounts for the significant viremia observed during acute HAV infection, but this does not explain the presence of virus in bile (48) or excretion of ...
10
Entry and Release of Hepatitis C Virus in Polarized Human Hepatocytes
... HCV entry into hepatocytes is a complex, multistep, and slow process (10). Two tight-junction proteins, claudin-1 (CLDN1) and occludin (OCLN), are among the many players involved in HCV entry (11, 12). Initial ...
16
Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes
... lipids. Hepatocytes are polarized epithelial cells that have basolateral (oriented toward the blood) and apical (oriented toward the bile) exosomal ...the human hepato- carcinoma cell line HepG2/C3A that ...
14
The role of gender and genetic polymorphisms in de novo choline synthesis
... collaborations throughout my time in the Zeisel lab. I would like to thank Dr. Mihai George Mehedint, Dr. Mihai Niculescu, and Corneliu Craciunescu for providing technical expertise as well as sharing colorful ...
144
Adenovirus Binding to Blood Factors Results in Liver Cell Infection and Hepatotoxicity
... significant implications for current efforts utilizing systemically applied Ad vectors for in vivo gene therapy. This model is also in conflict with recent papers by Smith et al. reporting that the KKTK motif present ...
14
Inducible nitric oxide synthase expression in chronic viral hepatitis Evidence for a virus induced gene upregulation
... tured human hepatocyte–derived ...in hepatocytes from pa- tients with CAHB, without relationship to the degree of hepatic inflammation, strongly supports the assumption that a similar HBx-mediated effect on ...
11
Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction
... of human primary hepatocytes after AAV transduction, MDA5 upregulation was only observed in primary hepatocytes from 6 of 12 subjects (Figure ...in human hepatocytes xenograft mice may ...
16
Hepatitis C Virus-Induced Changes in MicroRNA 107 (miRNA-107) and miRNA-449a Modulate CCL2 by Targeting the Interleukin-6 Receptor Complex in Hepatitis
... using human hepatocytes, clearly indicate FIG 5 miRNA-449a and miRNA-107 regulate IL-6-mediated CCL2 expression and STAT3 phosphorylation by targeting IL-6R and ...(A) Hepatocytes were transfected ...
11
Enhancement of recovery of Neisseria meningitidis by gelatin in blood culture media
... of human liver ...using human induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) as previously ...
9
Insulin like growth factor I binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver
... in human hepatoma cells (HEP-G2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own ...normal human hepatocytes, compared with HEP-G2 cells, have ...
7