and NS5A proteins
The hepatitis C virus NS4A protein: interactions with the NS4B and NS5A proteins.
... transfected with the indicated set of pcDLSR a plasmids encoding the tagged form of various HCV-H nonstructural proteins. The presence ( 1 ) or absence ( 2 ) of pcDLSR a /NS4A-HA and cotransfection of other ...
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Modulation of Hepatitis C Virus Genome Encapsidation by Nonstructural Protein 4B
... virus proteins directly involved in HCV ...and NS5A C465S) were identified, and introduction of these mutations into the chimera rescued virus production to various levels, suggest- ing a genetic ...
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Hepatitis C Virus F Protein Is a Short-Lived Protein Associated with the Endoplasmic Reticulum
... of NS5A (Fig. 5). Both core and NS5A proteins had previously been shown to localize predom- inantly to the ER membranes (3, 10, 15, 19, ...and NS5A, coimmunoprecipitation experiments failed to ...
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A Novel Hepacivirus with an Unusually Long and Intrinsically Disordered NS5A Protein in a Wild Old World Primate
... Research on HCV has been limited by the lack of a suitable animal model. The chimpanzee model uniquely permits studies of HCV infectivity and pathogenesis (73) but has considerable draw- backs, including expense, ...
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Hepatitis C Virus NS5A Disrupts STAT1 Phosphorylation and Suppresses Type I Interferon Signaling
... C-terminal NS5A plasmids or NS4B plasmids were cotransfected with pISRE-luc and pRL-TK into ...C-terminal NS5A proteins produced comparable degrees of ISRE signaling inhibition in both the GT1 and ...
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Regulation of Hepatitis B Virus Replication by the Phosphatidylinositol 3-Kinase-Akt Signal Transduction Pathway
... HCV NS5A protein interacts with the p85 subunit of PI3K and activates the PI3K-Akt signal transduction pathway (19, 31, 45, ...HCV NS5A protein might be involved in the inhibitory effect of HCV upon HBV ...
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Distinct Functions of NS5A in Hepatitis C Virus RNA Replication Uncovered by Studies with the NS5A Inhibitor BMS-790052
... of NS5A. To further investigate whether NS5A can be efficiently exchanged among replication complexes, a NS5A trans-complementation assay similar to that described by Jones et ...of NS5A ...
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Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
... of NS5A to the nucleus after disruption of the amphipathic nature of its N-terminal helix and the strict preservation of this motif in all known HCV isolates suggest that disruption of the AH may have significant ...
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Conserved Determinants for Membrane Association of Nonstructural Protein 5A from Hepatitis C Virus and Related Viruses
... of NS5A from GBV-B, GBV-C, and ...HCV, NS5A proteins from GBV-B, GBV-C, and BVDV possess membrane anchor domains within the N-terminal 27 to 33 amino ...truncated NS5A expression ...expressing ...
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Regulation of the Production of Infectious Genotype 1a Hepatitis C Virus by NS5A Domain III
... The NS5A proteins encoded by the JFH-1 and HJ3-5 viruses are identical, and the proteins expressed by these two RNAs each show a slight dominance of the faster migrating of the two major isoforms of ...
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Clinical and virological properties of hepatitis C virus genotype 4 infection in patients treated with different direct-acting antiviral agents
... the NS5A polymorphic position 58, T and P were observed in ...described NS5A resistance-associated substitution (RAS), Y93H/S, in Pt1, while Pt2 had emergent NS5A-L28F and NS5B-L204F (a novel RAS) at ...
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Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production
... Plasmids constructs were based on JFH-AM120 that has adaptive mutations previous reported (18) and JFH-AM120 based on the consensus sequence of HCV pJFH1, which was kindly provided by Dr. Wakita (11). JFH-AM120-EGFP and ...
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The NS5A/NS5 Proteins of Viruses from Three Genera of the Family Flaviviridae Are Phosphorylated by Associated Serine/Threonine Kinases
... HCV NS5A protein has also been shown in transiently transfected mammalian cells (22, ...HCV NS5A and of in vitro-phosphorylated TBE NS5 have indicated that all three proteins are phosphor- ylated ...
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Combination between different proteins and between proteins and yeast nucleic acid
... Suspensions of heat-coagulated rabbit globin, hurman-serum globulin and the protein component of tobacco mosaic virus were tested quantitatively at different pH values for their ability [r] ...
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NS5A Domain 1 and Polyprotein Cleavage Kinetics Are Critical for Induction of Double Membrane Vesicles Associated with Hepatitis C Virus Replication
... and NS5A completely blocked DMV biogenesis, arguing that this precursor plays an important role in the formation of the membranous HCV repli- cation ...or NS5A maturation to allow proper folding, ...
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Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
... NS proteins are involved in the virion assembly of Flaviviridae viruses (25, 29, 30, ...NS proteins to the LD-associ- ated membranes and that the NS proteins around the LDs participate in the ...
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Extraction of proteins and preliminary characterization of physicochemical properties in Toona sinensis fruit.
... The proteins from this plant possess specific physiological functions, which have attracted the attention of nutrilogical ...These proteins may reduce cholesterol levels (Higashi et ...
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Should NS5A inhibitors serve as the scaffold for all-oral anti-HCV combination therapies?
... Therapy against hepatitis C has been revolutionized in the past several years because of the tireless efforts of inves- tigators in academics and the pharmaceutical industry to develop highly efficacious DAA agents while ...
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Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: properties of the NS5A-associated kinase.
... of NS5A derived from HCV isolates of the 1a and distantly related 2a genotypes is ...of NS5A from the 1a isolate indicated that phosphorylation occurs predominantly on serine, with a minor fraction of ...
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Hepatitis C Virus Nonstructural Protein 5A Modulates the Toll-Like Receptor-MyD88-Dependent Signaling Pathway in Macrophage Cell Lines
... Immunoprecipitation and immunoblotting. Cells were seeded onto a six-well tissue culture plate 24 h before transfection. The plasmids were transfected by the lipofection method, and the cells were harvested at 48 h ...
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