Annulus Fibrosus
Effects of initial boost with TGF-beta 1 and grade of intervertebral disc degeneration on 3D culture of human annulus fibrosus cells
... of annulus tissue degeneration on the re-differentiation capacity of expanded AF cells in 3D polymer scaffolds, gene expression profiles were stratified by degeneration ...degenerated annulus ...
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TGF-β1 reduces the oxidative stress-induced autophagy and apoptosis in rat annulus fibrosus cells through the ERK signaling pathway
... Intervertebral disc degeneration: the role of the mitochondrial pathway in annulus fibrosus cell apoptosis induced by overload. Beta1 integrin inhibits apoptosis induced by cyclic stretc[r] ...
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Integrating Theoretical and Experimental Methods for Multi-Scale Tissue Engineering of the Annulus Fibrosus of the Intervertebral Disc
... the annulus fibrosus is predicated on a high degree of structural organization over multiple length scales: aligned bundles of collagen fibers reside within each lamella and the direction of alignment ...
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The involvement of interleukin 1 and interleukin 4 in the response of human annulus fibrosus cells to cyclic tensile strain: an altered mechanotransduction pathway with degeneration
... The intervertebral disc (IVD), comprising a central gela- tinous nucleus pulposus (NP) and the peripheral col- lagenous annulus fibrosus (AF), is a fibrocartilage pad which functions to provide stability to ...
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Original Article Bone marrow-derived mesenchymal stem cell composited with platelet rich plasma improved repair of annulus fibrosus rupture
... factor (PDGF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor (ILGF), which are ben- efit for speeding up wound and bone healing [11]. PRP promotes the ...
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Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells
... The lifetime prevalence of degenerative disc disease (DDD) is very high. Over 90% of over age 50 show radiographic signs of DDD in magnetic resonance imaging studies [1]. Degeneration of the intervertebral disc (IVD) is ...
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Development of a two-step protocol for culture expansion of human annulus fibrosus cells with TGF-β1 and FGF-2
... groups. Annulus fibrosus cell phenotypes were only marginally retained under the different protocols based on quantitative polymerase chain reaction ...
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Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture
... Under physiological conditions, nutrients diffuse in and out of the central disc through the cartilage endplate (CEP) and the annulus fibrosus (AF) [9]. Because the CEP contains many nutrient channels that ...
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Shape memory porous alginate scaffolds for regeneration of the annulus fibrosus Effect of TGF β3 supplementation and oxygen culture conditions
... Accepted Manuscript Shape-memory porous alginate scaffolds for regeneration of the annulus fibrosus - Effect of TGF-β3 supplementation and oxygen culture conditions Olivier Guillaume, An[r] ...
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Structural modelling of the annulus fibrosus - an anisotropic hyperelastic model approach at the lamellar level
... the annulus width in the radial direction ...whole annulus fibrosus represented as a simplified cylindrical ...the annulus bulge as well as the effect of the site-dependency of the fibrous ...
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Autophagy in rat annulus fibrosus cells: evidence and possible implications
... Figure 4 Autophagy incidence is partially rescued by 10% FBS. (a) The incidence of autophagy annulus fibrosus (AF) cells after re- fed with 10% fetal bovine serum (FBS) for six hours. The cells were ...
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Fluid-induced, shear stress-regulated extracellular matrix and matrix metalloproteinase genes expression on human annulus fibrosus cells
... The intervertebral disc (IVD) is composed of two dis- tinct components [1, 2], the central gelatinous nu- cleus pulposus (NP) and the surrounding outer annulus fibrosus (AF). Movement of the spine sub- ...
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MMP 2 mediates local degradation and remodeling of collagen by annulus fibrosus cells of the intervertebral disc
... By using RNAi, we showed that MMP-2 functions as the primary gelatinase and is used by AF cells for degrading the local surrounding matrix.. Notably, in the absence of MMP-2, collagen re[r] ...
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The Abnormal Annulus Fibrosus: Can We Infer the Acuteness of an Annular Injury?
... Although this article raises a number of questions, it is a valuable starting point for further investigation of the importance of these signal intensity abnormal- ities on T2-weighted images. It would be of interest to ...
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<p>Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway</p>
... All these results indicated that melatonin induced autophagy and inhibited apoptosis in AF cells of patients with DD.. In addition, the matrix metabolism is critical for the developme[r] ...
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A novel electrospun-aligned nanoyarn/three-dimensional porous nanofibrous hybrid scaffold for annulus fibrosus tissue engineering
... engineering scaffold for AF repair, we created a nanoyarn nanofibrous scaffold and a three-dimensional porous nanofiber/nanoyarn HS that partially mirrored several impor- tant fun[r] ...
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Biocompatibility and intradiscal application of a thermoreversible celecoxib loaded poly N isopropylacrylamide MgFe layered double hydroxide hydrogel in a canine model
... Chronic low back pain is a debilitating disorder associ- ated with intervertebral disc (IVD) degeneration [1]. As the exact pathogenesis is still poorly understood, current surgical and medical treatments aim at ...
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Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration
... AF: Annulus fibrosus; α -MEM: Minimum essential medium Eagle, alpha modification; CD: Chondrodystrophic; COL1A1: Collagen, type I, α 1; COL2A1: Collagen, type II, α 1; DMEM: Dulbecco ’ s modified Eagle ’ s ...
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High mechanical strain of primary intervertebral disc cells promotes secretion of inflammatory factors associated with disc degeneration and pain
... The human lumbar spine is a partially flexible structure with six degrees of freedom in motion that is capable of a wide variety of routine and athletic movements [3]. Under various circumstances, the lumbar spine can be ...
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A complex interaction between Wnt signaling and TNF α in nucleus pulposus cells
... Nucleus pulposus cells and annulus fibrosus cells were transferred to 24-well plates at a density of 3 × 10 4 cells/ well 1 day before transfection. Cells were co-transfected with 100 to 500 ng of ...
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