Automated ribotyping
Comparison of Rapid, Automated Ribotyping and DNA Macrorestriction Analysis of Burkholderia pseudomallei
... An automated ribotyping device (RiboPrinter) was used to determine the ribotypes of a collection of Burk- holderia pseudomallei ...EcoRI ribotyping was 100%, and the discrimination index was ...
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Characterization of Members of the Legionellaceae Family by Automated Ribotyping
... The automated ribotyping system with EcoRI restriction digestion has been shown to be a powerful tool for general genomic analysis of Legionella isolates ...
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Automated Ribotyping Provides Rapid Phylogenetic Subgroup Affiliation of Clinical Extraintestinal PathogenicEscherichia coli Strains
... of ribotyping compared to MLEE typing, only one ribotype pattern, containing ECOR 24, 26, 31, and 37, carried a risk of erroneous phylogenetic group attri- ...the automated ribotyping method has the ...
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Distinguishing Species of the Burkholderia cepacia Complex and Burkholderia gladioli by Automated Ribotyping
... France). Automated ribotyping was per- formed under the conditions recommended by the manufacturer of the Ribo- printer microbial characterization system (Qualicon Europe ...
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Molecular Surveillance of European Quinolone Resistant Clinical Isolates of Pseudomonas aeruginosa andAcinetobacter spp Using Automated Ribotyping
... The results suggest correspondence between the major ribo- groups of sitafloxacin-resistant isolates identified in the present study and the previously identified major clones of P. aerugi- nosa and A. baumannii. First, ...
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Automated Ribotyping of Vancomycin Resistant Enterococcus faecium Isolates
... of automated ribotyping data, can be a source of ambiguity, since it is known that overloading reduces DNA fragment mobility in agarose ...between automated categorization and manual categorization ...
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Comparison of Automated Ribotyping to Pulsed Field Gel Electrophoresis for Genetic Fingerprinting ofStreptococcus pneumoniae
... The copyright holder for this preprint which was not certified by peer review is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.. It is made a[r] ...
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Subtyping of Salmonella enterica Serotype Enteritidis Strains by Manual and Automated PstI SphI Ribotyping
... for automated ribotyping compared with that obtained by use of Bionumerics software to score every ...manual ribotyping gen- erally assume that a single band difference is significant, allow- ing ...
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Comparison of DNA Fingerprinting Methods for Use in Investigation of Type E Botulism Outbreaks in the Canadian Arctic
... Fingerprinting analysis of RAPD patterns of C. botulinum type E has been traditionally performed by visual examination, presumably because of the presence of a large number of low-molecular-weight fragments and the ...
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Computer Assisted Analysis and Epidemiological Value of Genotyping Methods for Campylobacter jejuni andCampylobacter coli
... (PFGE), automated ribotyping, and amplified fragment length polymorphism (AFLP) fingerprinting were ...and ribotyping discriminated 26 different ...
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Emergence of Extended Spectrum β Lactamase (CTX M 9) Producing Multiresistant Strains of Salmonella enterica Serotype Virchow in Poultry and Humans in France
... Resistance transfer determination and plasmid analysis. A resistance transfer experiment was carried out on liquid media with E. coli J5 (rif) as the recipient strain. E. coli transconju- gants pROU-1, p2437-1, p2731-1, ...
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Recognition of Two Groups of Methicillin ResistantStaphylococcus aureus Strains Based on Epidemiology, Antimicrobial Susceptibility, Hypervariable Region Type, and Ribotype in Finland
... (10). Ribotyping with concomitant analysis of the mec DNA thus provides information on the potential differences in the genomic background or on the mec DNA of epidemic and sporadic ...
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Characterization of Extended Spectrum β Lactamase Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods
... study, ribotyping revealed an identical pattern ...that ribotyping is of limited value in the epidemiological analysis of these Salmonella ...ever, ribotyping with hybridization with the IS200 probe ...
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Comparison of ribotyping with conventional methods for the type identification of Enterobacter cloacae
... By the combined typing methods, isolates from the same incident were considered representatives of a single strain if they had the same 0 serotype reactions and biotype and did not diffe[r] ...
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Quantitative antibiogram typing using inhibition zone diameters compared with ribotyping for epidemiological typing of methicillin resistant Staphylococcus aureus
... Dendrogram of epidemiologically unrelated MRSA strains obtained by antibiogram cluster analysis quantitative antibiogram typing, resistance profiles, and results of ribotyping... tained [r] ...
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Persistence of a Distinct Corynebacterium diphtheriae Clonal Group within Two Communities in the United States and Canada Where Diphtheria Is Endemic
... electrophoresis, ribotyping, and random amplified polymorphic DNA showed that strains with distinct molecular subtypes have persisted in the United States and Canada for at least 25 ...
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Comprehensive ribotyping scheme for heat stable serotypes of Campylobacter jejuni
... 16S ribotyping can resolve the epidemiological un- certainty associated with nonserotypeable isolates of ...16S ribotyping as a routine epidemio- logical tool will therefore require its own set of reference ...
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Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms
... Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR AP-PCR fingerprinting, and the study of mapped[r] ...
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Multicenter Evaluation of the Verigene Clostridium difficile Nucleic Acid Assay
... The Verigene Clostridium difficile Nucleic Acid test (Verigene CDF test) (Nanosphere, Northbrook, IL) is a multiplex qualitative PCR assay that utilizes a nanoparticle-based array hybridization method to detect C. ...
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Molecular Subtyping Methods for Detection of Salmonella enterica Serovar Oranienburg Outbreaks
... Since the potential of Salmonella contamination in seafood products, which are distributed on a nationwide basis, remains a national threat, it is therefore recommended that every med- ical facility that isolates ...
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