B. pseudomallei
PCR-based Methodologies Used to Detect and Differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis
... 2010). B. pseudomallei isolated from clinical samples accounted for 50% of community-acquired septicemias during the rainy seasons in northeastern Thailand (Chaowagul et ...
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Comparison of Diagnostic Laboratory Methods for Identification of Burkholderia pseudomallei
... Burkholderia pseudomallei, the cause of melioidosis, difficult to recognize in the diagnostic microbiology ...presumptive B. pseudomallei strains using a collection of Burkholderia species drawn from ...
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Detection of Burkholderia pseudomallei DNA in patients with septicemic melioidosis
... to B. pseudomallei was ...of B. pseudomallei and none of the other bacteria tested were ...of B. pseudomallei in buffy coat specimens by PCR was shown to be comparable to the ...
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Assessment of a DNA vaccine encoding Burkholderia pseudomallei bacterioferritin
... Groups of 20 Balb/c mice were intradermally (ID) immunised on day 0, day 14 and day 28 with either the DNA construct pBps190 or the pcDNA3.1start vector (8) that did not contain the bacterioferritin gene. Ten mice from ...
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Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer
... for B. pseudomallei to be misidentified as ...include B. pseudomallei spectra, but it is not FDA approved nor has it been evaluated in a clinical context ...of B. pseudomallei ...
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The role of dendritic cells in the dissemination of Burkholderia pseudomallei infection
... during B. pseudomallei infection is yet to be ...during B. pseudomallei infection could potentially contribute to the mechanisms driving the development of septic shock in ...to B. ...
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Development and Evaluation of a Real Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei
... misidentify B. pseudomallei as other bacteria such as Pseudo- monas ...to B. pseudomallei may result in seroconversion (6, 27, ...of B. pseudomallei to many of the affordable ...
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Genetic Diversity of Burkholderia pseudomallei Isolates in Australia
... that B. pseudomallei is a relatively young species and, since their original distribution over southeast Asia and northern Australia, sufficient time has not elapsed for all of the ancestral alleles to ...
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Burkholderia pseudomallei: Challenges for the Clinical Microbiology Laboratory
... agar, B. pseudomallei selective agar (BPSA), or ...polymyxin B disk may also be placed in the first quadrants of blood agar plates to help select for Burkholderia ...
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groEL Encodes a Highly Antigenic Protein in Burkholderia pseudomallei
... Besides laboratory diagnosis, the GroEL protein can be used for immunization in those patients at high risk of developing B. pseudomallei infections. Chaperonins are immunodominant proteins in microbial ...
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Successful treatment outcome of primary melioidosis pneumonia a case report from Bangladesh
... Background: Melioidosis is endemic in tropical Australia and Southeast Asian countries and its causative organism Burkholderia pseudomallei is a recognized cause of pneumonia in these regions. Recent isolation of ...
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Prevalence and Sequence Diversity of a Factor Required for Actin Based Motility in Natural Populations of Burkholderia Species
... predicted B. pseudomallei BimA sequences that resemble the prototypic BimA Bp from K96243, the predicted proline-rich motifs and tandem WH2 domains were ...of B. pseudomallei strain 14 ...
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Seronegative Bacteremic Melioidosis Caused by Burkholderia pseudomallei with Ambiguous Biochemical Profile: Clinical Importance of Accurate Identification by 16S rRNA Gene and groEL Gene Sequencing
... of B. pseudomallei and that of ...of B. pseudomallei and discrimination between B. pseudomallei and ...of B. pseudomallei with an ambiguous biochemical profile ...
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Calprotectin as a Biomarker for Melioidosis Disease Progression and Management
... by B. pseudomallei and examine the possibility of using CALP as a general biomarker for clinical responses during the acute and eradication phases of antibiotic treatment in human cases of ...
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The Brief Case: A Traveler’s Tale—Burkholderia pseudomallei Infection in a Cystic Fibrosis Patient
... as B. pseudomallei was not clinically ...to B. pseudomallei but is nonpathogenic in ...of B. pseudomallei by the Bruker MALDI-TOF MS has been well described in the literature (1, ...
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Novel Pan Genomic Analysis Approach in Target Selection for Multiplex PCR Identification and Detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia Complex Species: a Proof of Concept Study
... Since the first bacterial genome was published in 1995 (16), more than 1,000 prokaryotic genomes have been sequenced. As with other virulent or emerging bacterial pathogens (34, 45, 51), complete genome sequences of 23 ...
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Accurate identification and epidemiological characterization of Burkholderia cepacia complex : an update
... Similarly, rpsU was recognized to identify diferent spe- cies among the Burkholderia genus [48]. Frickmann et al. [48] has employed rpsU sequencing method to com- pare Burkholderia strains of known identity from ATCC ...
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Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update
... 16S rRNA sequencing is one important option for differ- entiating non-Burkholderia spp. from Burkholderia spp. [49]. The 16S rRNA similarity between Burkholderia spp. and non-Burkholderia spp. is given in Table 3. In a ...
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Epidemiological Tracking and Population Assignment of the Non-Clonal Bacterium, Burkholderia pseudomallei
... Burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining ...distinct B. pseudomallei populations have been ...
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Within Host Evolution of Burkholderia pseudomallei over a Twelve Year Chronic Carriage Infection
... MSHR6686 has also shed genes encoding putative lipoprotein capsule biosynthesis and modification, chemotaxis, motility and quorum sensing, fatty acid biosynthesis, DNA methylation, thia- mine biosynthesis, antibiotic ...
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