BA/F3
The OTT MAL fusion oncogene activates RBPJ mediated transcription and induces acute megakaryoblastic leukemia in a knockin mouse model
... We next assessed whether OTT-MAL activation of RBPJ tran- scription is involved in the proliferation of 6133 AMKL cells. We performed coimmunoprecipitation to confirm that endogenous OTT-MAL but not MAL interacted with ...
14
Transposon-mediated generation of BCR-ABL1 -expressing transgenic cell lines for unbiased sensitivity testing of tyrosine kinase inhibitors
... generating Ba/F3 cells with single insertions of mutant BCR-ABL1 constructs to provide a basis for adequate in vitro sensitivity testing of ...SB-transfected Ba/F3 cells with gating for cell ...
12
Biologically active fibroblast growth factor 1 tagged with various epitopes
... Mitogenic activity of FGF1s towards FGFR1c-Ba/F3 cells. (a) FGFR1c-Ba/F3 cells were cultivated for 48 h with the indicated concentrations of FGF1s in the presence of 10 μg/ml heparin (closed ...
5
Combination therapy with copanlisib and ABL tyrosine kinase inhibitors against Philadelphia chromosome-positive resistant cells
... study, Ba/F3 BCR-ABL (T315I) mutant cells were injected subcutaneously or ...of Ba/ F3 BCR-ABL (T315I) mutant cells in vivo to a greater extent than the vehicle control (PBS) (P < ...
11
Expression of GADS enhances FLT3-induced mitogenic signaling
... used Ba/F3-FLT3-ITD/empty vector and BaF3-FLT3-ITD/GADS ...in Ba/F3-FLT3- ITD/GADS cells. Furthermore, Ba/F3-FLT3-ITD cells expressing GADS displayed significantly higher STAT5 ...
13
Asymmetric kinase dimer formation is crucial for the activation of oncogenic EGFRvIII but not for ERBB3 phosphorylation
... [8,9,12]. Ba/F3 cells require IL-3 for survival and intro- duction of oncogenes into these cells confer cytokine- independence on these cells ...employed Ba/F3 cells to test the physiological ...
7
A chimeric receptor/oncogene that can be regulated by a ligand in vitro and in vivo
... given Ba/F3 cells expressing wild-type EPO receptors also develop a fatal neoplasm when treated with EPO, but the accumulation of cells in different organs is different than that of Ba/F3 ...
9
Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN
... in Ba/F3 cells expressing MPL YF variants harboring loss of ...in Ba/F3 cells stably expressing MPL-YF variants demonstrates that all three intracellular tyrosines play a role in supporting ...
14
Retroviral Vector Design for Gene Therapy of Cancer: Specific Inhibition and Tagging of BCR-ABLp190 Cells
... (B) Ba/F3+p210 cells were infected with the virus encoding BCR-ABLP'90 antisense transcripts and the truncated human CD5 surface marker. Infected cells (left panel) were assessed by thei[r] ...
9
Gd<sub>2</sub>O<sub>3</sub> nanoparticles in hematopoietic cells for MRI contrast enhancement
... Abstract: As the utility of magnetic resonance imaging (MRI) broadens, the importance of having specific and efficient contrast agents increases and in recent time there has been a huge development in the fields of ...
8
The βc Component of the Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF)/Interleukin 3 (IL-3)/IL-5 Receptor Interacts with a Hybrid GM-CSF/Erythropoietin Receptor to Influence Proliferation and β-Globin mRNA Expression
... hybrid receptor, GMER (9), which consists of the extracellular and transmembrane regions of hGMRa and the cytoplasmic domain of the mu- rine (m) EpoR into Ba/F3 cells (10), a murine IL-3[r] ...
8
The Mouse Mammary Tumor Virus Transcription Enhancers for Hematopoietic Progenitor and Mammary Gland Cells Share Functional Elements
... expression of the luciferase reporter gene (luc). The MMTV enhancer element is represented by an open box, with an arrow indicating the positive orientation of the fragment. The IL-3-dependent mouse progenitor cell lines ...
5
In vitro modeling to determine mutation specificity of EGFR tyrosine kinase inhibitors against clinically relevant EGFR mutants in non-small-cell lung cancer
... using Ba/F3 cells harboring wild type EGFR and EGFR ...the Ba/F3 cells with annexin V-APC and propidium iodide after 48 h of EGFR-TKI treatment ...both Ba/ F3 cells harboring ...
15
Identification of amino acids in the transmembrane and juxtamembrane domains of the platelet-derived growth factor receptor required for productive interaction with the bovine papillomavirus E5 protein.
... A mutant PDGF b receptor containing a glutamic acid or an alanine in place of lysine 499 (PRK499E or PRK499A, respec- tively) also was defective for cooperating with the E5 protein. None of numerous independently derived ...
10
Stable expression of small interfering RNA sensitizes TEL PDGFβR to inhibition with imatinib or rapamycin
... fusions BCR-ABL–TEL-PDGFbR–mediated human leukemias, but resistance may develop. The unique fusion junctions of these molecules are attractive candidates for molecularly targeted therapeutic intervention using RNA ...
9
Axitinib and sorafenib are potent in tyrosine kinase inhibitor resistant chronic myeloid leukemia cells
... higher efficacy in downregulating the phosphorylation of ABL (Y245) and CRKL (Y207) in Bcr-Abl wt and Bcr- Abl T315I cells compared to SF indicating that AX has a higher impact on the Bcr-Abl activity (Figs. 1d and ...
7
Comparing Phenolic Composition of Cabernet Gernischet Wines between Rain-shelter Cultivation and Open-field Cultivation
... that anthocyanins synthesise Figure 2, the content of F3’5’H branch pathway was higher in rain-shelter cultivation, but the F3'H has different expression in two vintages; the ratio of F3[r] ...
11
Zebra finches and Dutch adults exhibit the same cue weighting bias in vowel perception
... Based on their results, different hypotheses have been formulated to account for cue weighting differences between languages and ages. The ‘‘Developmental Weighting Shift’’ (Nittrouer 1996) hypothesis postulates that ...
7
F3/contactin and TAG1 play antagonistic roles in the regulation of sonic hedgehog induced cerebellar granule neuron progenitor proliferation
... of F3-fc binding, we tested the ability of F3-fc to suppress SHH-induced proliferation of NrCAM-null ...of F3-fc failed to suppress this increase in proliferation ...with F3 on GNPs and is ...
11
A phylogenetic examination of the primary anthocyanin production pathway of the Plantae
... Monocots diverged from eudicots 75–100 million years ago (Kellogg 2001). As the monocots diverged, our data suggest that the components of the anthocyanin production pathway, although conserved functionally, began to ...
10