Bacillus anthracis protective antigen
Immunogenicity of Bacillus anthracis Protective Antigen Domains and Efficacy of Elicited Antibody Responses Depend on Host Genetic Background
... to Bacillus anthracis protective antigen (PA), a component of anthrax toxin, mediate protection against ...elicit protective and nonprotective ...and protective efficacies of the ...
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Computational Improvement of Small-Molecule Inhibitors of Bacillus anthracis Protective Antigen Activation through Isostere-Based Substitutions.
... Abstract: There has recently been interest in the development of small-molecule inhibitors of the oligomerization of Bacillus anthracis protective antigen for therapeutic use. Some of the ...
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Phase I Study Evaluating the Safety and Pharmacokinetics of MDX-1303, a Fully Human Monoclonal Antibody against Bacillus anthracis Protective Antigen, in Healthy Volunteers
... MDX-1303 (Valortim) is a fully human monoclonal antibody (hMAb) with a high affinity for Bacillus anthracis protective antigen (PA). MDX-1303 binds to PA and interferes with the activity of ...
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Chimeric Influenza Virus Hemagglutinin Proteins Containing Large Domains of the Bacillus anthracis Protective Antigen: Protein Characterization, Incorporation into Infectious Influenza Viruses, and Antigenicity
... the Bacillus anthracis protective antigen (PA) were inserted into an influenza A virus hemagglutinin glycoprotein (HA), and the chimeric proteins were functionally characterized and ...
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Enzyme-linked immunosorbent assay using a recombinant baculovirus-expressed Bacillus anthracis protective antigen (PA): measurement of human anti-PA antibodies.
... We developed an antigen capture enzyme-linked immunosorbent assay (ELISA) which does not require purified protective antigen (PA) for detection of human antibodies to Bacillus anthracis [r] ...
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Serum-Mediated Cleavage of Bacillus anthracis Protective Antigen Is a Two-Step Process That Involves a Serum Carboxypeptidase
... associated activities remains to be defined. On the basis of earlier studies that associ- ated furin-like digestion with protection, we believe that this activity may in fact contribute to the host response to anthrax. ...
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In Vitro Study of the Toxicity Effects of Bacillus anthracis Protective Antigen
... by Bacillus anthracis ...for Bacillus anthracis pathogenicity [1, ...(EF), Protective Antigen (PA), and Lethal Factor (LF)) are encoded by pXO1 or PBA1 plasmids [3, ...4]. ...
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Internalization of a Bacillus anthracis Protective Antigen-c-Myc Fusion Protein Mediated by Cell Surface Anti-c-Myc Antibodies
... If the PA-c-Myc protein uses the 9E10 antibody to enter and kill hybridoma cells, the addition of the c-Myc peptide should compete with PA-c- Myc binding and thus protect cells from toxi[r] ...
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Expression of the protective antigen of bacillus anthracis in attenuated salmonella : a potential oral anthrax vaccine
... toxin. Protective Antigen, can protect against challenge (Ivins & W elkos, ...the protective antigens have not been identified, or a response to a number o f antigens are required to stimulate ...
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Fatal community acquired Bacillus cereus pneumonia in an immunocompetent adult man: a case report
... B. anthracis toxin ...and protective antigen gene (PAg) (Genbank ...of Bacillus anthracis (JNBP01251) was provided by the Gifu Type Culture Collection, Graduate School of Medicine, Gifu ...
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Activity of the Bacillus anthracis20 kDa protective antigen component
... Spores were prepared from B. anthracis Ames strain (pXO1+, pXO2+). Briefly, 5% sheep blood agar (SBA) plates were inoculated with B. anthracis Ames spores and incubated overnight at 35°C. Several isolated ...
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Phase 1 Study of a Recombinant Mutant Protective Antigen of Bacillus anthracis
... A phase 1 study of a recombinant mutant protective antigen (rPA) vaccine was conducted in 186 healthy adults aged 18 to 45 years. Volunteers were randomized to receive one of three formulations of rPA ...
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Detection of Bacillus anthracis DNA by LightCycler PCR
... of Bacillus anthracis, but a definitive identification may take 24 to 48 h or longer and may require that specimens be referred to another ...B. anthracis contain two plasmids (pX01 and pX02) with ...
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Bacillus anthracis Diversity in Kruger National Park
... MLVA. The MLVA strain typing procedure is described in detail elsewhere (9). Briefly, a single colony of B. anthracis was “heat lysed” in TE buffer (10 mM Tris [pH 8.0], 1 mM EDTA) at 95°C for 20 min. Cellular ...
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Characterization of Bacillus cereus Isolates Associated with Fatal Pneumonias: Strains Are Closely Related to Bacillus anthracis and Harbor B anthracis Virulence Genes
... ments. Three microliters of each product was used as a template in subsequent selective amplifications using the ⫹ 1/ ⫹ 1 primer combination of 6-carboxyfluo- rescein-labeled EcoRI-C (5 ⬘ -GTAGACTGCGTACCAATTCC-3 ⬘ ) and ...
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Whole genome phylogeny of Bacillus by feature frequency profiles (FFP)
... In contrast to the FFP analysis based on the whole genome data, our phylogenetic analysis based on single gene data (16S rRNA gene, GyrB and AroE) were unable to clearly distinguish between Bacillus species ...
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Identifying experimental surrogates for Bacillus anthracis spores: a review
... behavior in release experiments. The spores of the B. cereus group have similar ratios of length to width and similar diameters, whereas the spores of B. atrophaeus are smaller and those of B. megaterium are larger ...
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The Genetic Diversity and Evolution of Francisella tularensis with Comments on Detection by PCR
... Major, Bacillus anthracis, Yersinia pestis, Francisella tularensis, and Varicella zoster virus) and another assay with a reverse transcriptase for RNA based viruses (Ebola virus, Lassa fever virus, Rift ...
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Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique
... ground fluorescence, the mean of a set of background voltages; bacterial fluorescence and autofluorescence, the maximum of the set of fluorescence voltages for a bacterium; bacterial sig[r] ...
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Improved Discrimination of Bacillus anthracis from Closely Related Species in the Bacillus cereus Sensu Lato Group Based on Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... acillus anthracis is a member of the Bacillus cereus sensu lato ...related Bacillus species: B. cereus, B. anthracis, ...B. anthracis from ...B. anthracis can cause fatal anthrax ...
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