Bacterial 16S rRNA
Absence of Spiroplasma or Other Bacterial 16S rRNA Genes in Brain Tissue of Hamsters with Scrapie
... for 16S rRNA gene bacterial sequences in DNA from the well-characterized ham- ster 263K scrapie model, which produces predictably high con- centrations of TSE infectivity in the brains of clinically ...
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Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro
... using 16S rRNA sequencing ...71 bacterial 16S rRNA genes in xenic algal suspension identified common environmental microbiota, one strain belonging to the class Alphaproteobacteria, 17 ...
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C:N ratio drives soil actinobacterial cellobiohydrolase gene diversity
... differences in GH48, bacterial 16S rRNA and fungal ITS gene composition across sites and.. land uses, ordination by principal coordinate PCO analysis was carried out..[r] ...
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Massive Parallel Sequencing Provides New Perspectives on Bacterial Brain Abscesses
... of bacterial brain abscesses using universal amplifi- cation of the bacterial 16S rRNA gene, followed by Sanger se- quencing of cloned amplicons, have revealed that only a fraction of the ...
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Bacteriological incidence in pneumonia patients with pulmonary emphysema: a bacterial floral analysis using the 16S ribosomal RNA gene in bronchoalveolar lavage fluid
... the bacterial 16S rRNA ...using bacterial 16S rRNA gene sequences that we used in this study may not be able to detect bacterial phylo- types that present with ,1% of the ...
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Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae).
... isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and MRS (de Man, Rogosa, and Sharpe) agar, and then randomly select colonies from each plate and stocked them to ...
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Study and exploitation of diverse soil environments for novel natural product discovery using metagenomic approaches
... general bacterial population recovery enabled evaluation of differences in the DNA extraction efficiency and potential inhibition caused by the co-extracted ...recovering bacterial DNA, while the original ...
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SSCP Analysis of V3 Region of 16S rRNA for the Characterization of Rumen Microbiome of Goat
... of bacterial 16S rRNA gene and V3 region of 16S rRNA are given in table ...of 16S rRNA was performed by thermal cycler (Mastercycler EP, Eppendorf) using the program: ...
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Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... the bacterial 16S rRNA and fungal 18S rRNA, were evaluated with reference bacterial and fungal strains, clinical isolates, and direct blood cul- ture bottle ...
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Interactive network configuration maintains bacterioplankton community structure under elevated CO2 in a eutrophic coastal mesocosm experiment
... Samples collected by 0.2 µm polycarbonate filters as de- scribed above were washed with PBS buffer and then cen- trifuged at 9600 g to obtain a cell pellet. A previously de- scribed DNA extraction protocol (Francis et ...
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New Nodule Type Found in the Lungs of Pomacea canaliculata, an Intermediate Host of Angiostrongylus cantonensis
... Both bacterial 16S rRNA and eukaryotic 18S rRNA were amplified by PCR and sequenced by the Illu- mina MiSeq platform (Origin-gene Biomedical Technology ...the bacterial 16S ...
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Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos
... of 16S rRNA genes was employed to investigate the optimized primer set which could be used to improve the determination of the microbial diversity of ...evaluate bacterial 16S rRNA gene ...
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The significance of oral streptococci in patients with pneumonia with risk factors for aspiration: the bacterial floral analysis of 16S ribosomal RNA gene using bronchoalveolar lavage fluid
... the bacterial 16S rRNA genes, and the sensitivity of the primers was approximately 92 % of the total bacterial species regis- tered in the Ribosomal Database Project II database, whereas no ...
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Elevation gradient of soil bacterial communities in bamboo plantations
... The 16S rRNA gene clone libraries were constructed as described (Lin et ...The bacterial 16S rRNA genes were amplified by PCR with the primer set 27F and 1492R (Lane ...
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PREDetector 2.0: Online and Enhanced Version of the Prokaryotic Regulatory Elements Detector Tool
... the bacterial 16S OTUs a representative sequence of each OTU was aligned to a termite specific database DictDb_v3 (Mikaelyan et ...bias, bacterial and protist datasets were rarefied resulting in 32 ...
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Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing
... PCR was performed in a 25 µL final volume, containing 2.5 µL of 10X buffer (10 mM Tris-HCl, 50 mM KCl), 0.2 µL of 25 mM dNTP, 1.0 µL of 50 mM MgSO4, 0.2 µL of Taq High Fidelity (Platinum ® Taq DNA Polyme- rase, Life ...
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Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis
... or 16S rRNA gene analysis does not necessarily mean that the bacterium has high metabolic activ- ...of 16S rRNA has previously been used to detect such active bacteria (31), but dysbiosis ...
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Identification of YbeY Protein Interactions Involved in 16S rRNA Maturation and Stress Regulation in Escherichia coli
... (including 16S rRNA along with ribosomal proteins S2, S7, S11, and S18; PDB accession number or code 1X18) ...containing rRNA segments, suggesting that the charged rRNA likely contributes to ...
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Metagenomic analysis of basal ice from an Alaskan glacier
... Samples of exposed basal ice at the terminus of the Mat- anuska Glacier (Additional file 1: Figure S1) were col- lected in July 2013 using an electric chainsaw (61.776 N 147.761 W, 510-m elevation). The glaciological and ...
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Prediction and Modeling of the Structure of 16S rRNA
... prokaryotic 16S rRNA alignment (Maidak, Cole et ...the 16S rRNA (Kalurachchi and Nikonowicz, 1998) revealed a base triple at these ...the 16S [595(596:644)] and 23S [1072(1092:1099)] ...
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