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Bacterial identification using the 16S rDNA gene

Sequence Based Identification ofMycobacterium Species Using the MicroSeq 500 16S rDNA Bacterial Identification System

Sequence Based Identification ofMycobacterium Species Using the MicroSeq 500 16S rDNA Bacterial Identification System

... Rapid identification of Mycobacterium ...rapid identification with a turnaround time of less than 1 day, but these assays can only test for one species at a time, and probes are available for only four ...

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High-throughput screening of bacterial pathogens in clinical specimens using 16S rDNA qPCR and fragment analysis

High-throughput screening of bacterial pathogens in clinical specimens using 16S rDNA qPCR and fragment analysis

... delivered bacterial species identification with a suf- ficient degree of certainty if mixed samples did not contain more than 2 different ...the 16S rDNA qPCR and fragment analysis workflow is ...

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Intergenic 16S rRNA gene (rDNA) 23S rDNA sequence length polymorphisms in members of the family Legionellaceae

Intergenic 16S rRNA gene (rDNA) 23S rDNA sequence length polymorphisms in members of the family Legionellaceae

... rapid identification of such eubacteria as Listeria, Staphy- lococcus, and Salmonella ...PCR using primers directed at conserved sequences of the 16S and 23S ...

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16S rDNA Identification and molecular characterization of xanthomonadaceae nml 03 0222 bacterial isolates from agricultural soil of jharkhand

16S rDNA Identification and molecular characterization of xanthomonadaceae nml 03 0222 bacterial isolates from agricultural soil of jharkhand

... The bacterial microorganisms always played an important role in increasing soil fertility of agricultural ...of bacterial identification have resulted in major drawbacks as they are extremely slow ...

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Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

... of bacterial origin, due to higher stocking density, high organic matter levels and poor quality of the aquatic environment that causes high mortality rates during ...The identification of pathogenic ...

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Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)

Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)

... four gene regions investigated herein showed overlap between within-species and among-species K2P values using data set 1 (Figure ...distances using data set 3, which only includes 256 sequences ...

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Performance of 16s rDNA primer pairs in the study of rhizosphere and endosphere bacterial microbiomes in metabarcoding studies

Performance of 16s rDNA primer pairs in the study of rhizosphere and endosphere bacterial microbiomes in metabarcoding studies

... Many bacterial 16S rDNA primer pairs also exhibit high affinity for non-target DNA such as plastid (mostly chloroplast) DNA and mitochondrial ...plant-associated bacterial communities ...

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Bacterial community dynamics in a swine wastewater anaerobic reactor revealed by 16S rDNA sequence analysis

Bacterial community dynamics in a swine wastewater anaerobic reactor revealed by 16S rDNA sequence analysis

... was the most dominant phylum in the feedstock samples while Bac- teroidetes and Firmicutes were relatively abundant in the digester samples. Interestingly, although Proteobacteria became relatively abundant on day 17 ...

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Contraception: Influence on vaginal microbiota and identification of vaginal lactobacilli using MALDI-TOF MS and 16S rDNA sequencing

Contraception: Influence on vaginal microbiota and identification of vaginal lactobacilli using MALDI-TOF MS and 16S rDNA sequencing

... with 16S rDNA sequencing applied to identify lactobacilli isolates from vaginal samples at the species ...of 16S rDNA sequencing and MALDI-TOF MS revealed a new species associated with the ...

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A molecular approach to the identification of actinomycetes producing antimicrobial activities strains by sequencing of the 16S rDNA

A molecular approach to the identification of actinomycetes producing antimicrobial activities strains by sequencing of the 16S rDNA

... molecular identification by sequencing the 16S rDNA ...of 16S rDNA fragments by PCR technique (Polymerase Chain Reaction), sequencing of the amplified fragments and comparison of ...

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Identification and Typing of Leptospira spp. by Restriction Fragment Length Polymorphism (RFLP) of 16S Gene rDNA and Pulsed-field Gel Electrophoresis (PFGE)

Identification and Typing of Leptospira spp. by Restriction Fragment Length Polymorphism (RFLP) of 16S Gene rDNA and Pulsed-field Gel Electrophoresis (PFGE)

... Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA; a new rapid method of serovar identification. J Clin Micro- biol 1992; 30: 1696–1702. [25] Collares-Pereira M, Korver H, Cao Thi BV, Santos-Reis ...

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Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences

Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences

... chitinase using colorimetric method, and molecular identification of bacteria based on 16S rDNA ...on 16S rDNA sequences, isolate of PBK 2 was identified as Acinetobacter ...

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Standard operating procedure for optimal identification of mycobacteria using 16S rRNA gene sequences

Standard operating procedure for optimal identification of mycobacteria using 16S rRNA gene sequences

... the identification (Figures 1 and 2) is evidently in contrast to the opinion of most mi- crobiologists using genetic sequencing for the speciation of ...the identification itself is regarded as a ...

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Characterization of the Dominant Bacterial Communities Associated with Terrestrial Isopod Species Based on 16S rDNA Analysis by PCR DGGE

Characterization of the Dominant Bacterial Communities Associated with Terrestrial Isopod Species Based on 16S rDNA Analysis by PCR DGGE

... the bacterial community that they ...the bacterial community associated with 11 specimens of terrestrial isopods belonging to six species using a Culture independent approach ...

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16S Ribosomal DNA Typing for Identification of Pathogens in Patients with Bacterial Keratitis

16S Ribosomal DNA Typing for Identification of Pathogens in Patients with Bacterial Keratitis

... of 16S rDNA appears to be highly sensitive and specific for the identification of bacterial pathogens in the setting of ker- ...potential bacterial pathogens of the ...The 16S ...

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Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene

Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene

... psittaci were described for PCR by using two primer sets (lA-lB and 2A-2B) derived from the 16S rRNA sequence of.. Chlamydia spp.[r] ...

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Unraveling bacterial fingerprints of city subways from microbiome 16S gene profiles

Unraveling bacterial fingerprints of city subways from microbiome 16S gene profiles

... “genus” respectively; note that RF calculates these rates internally by using the out-of-bag error of samples. Re- sults for each city are presented in Table 3. The error rate for “genus” was somewhat elevated ...

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Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification.

Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification.

... analysis using 97% ...Pad using the One-way ANOVA for between group ...= bacterial 16S rRNA gene region V1 to V2, V1V9 = bacterial full-length 16S rRNA gene, LO = ...

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Signature region within the 16S rDNA sequences of Aeromonas popoffii

Signature region within the 16S rDNA sequences of Aeromonas popoffii

... Abstract To identify a group of eight Aeromonas strains of our collection showing ribotyping patterns similar to those described for the species Aeromonas popoffii, 16S rRNA gene sequence analysis was ...

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Evaluation of Real Time 16S rDNA PCR and Pyrosequencing for Routine Identification of Bacteria in Joint Fluid and Tissue Specimens

Evaluation of Real Time 16S rDNA PCR and Pyrosequencing for Routine Identification of Bacteria in Joint Fluid and Tissue Specimens

... 16S rDNA PCR and sequencing are powerful tools for bacterial detection and identification, although their routine use is not currently widespread in the field of clinical ...makes 16S ...

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