BACTERIOPHAGE T7
Distinguishing Between Selection and Population Expansion in an Experimental Lineage of Bacteriophage T7
... of bacteriophage T7, designated “WT,” RESULTS was evolved for 2500 ...of bacteriophage T7: A total of ple populations are designated CW100, CW400, and ...
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Evidence for bacteriophage T7 tail extension during DNA injection
... Fractionation of a lysate of bacteriophage T7- infected E. coli. Particles in a 6-liter lysate of bacteriophage T7-infected E. coli BL21 were precipitated with polyethylene glycol, clarified ...
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Replicative Intermediates of Bacteriophage T7 Deoxyribonucleic Acid
... 3H-Thymidine 15.6 Ci/mmole, Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest After infection with bacteriophage T7, parental and newly synthesized deoxyribonucleic acid [r] ...
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Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.
... When extracts were incubated at 30°C and the intracellular DNA was subsequently extracted and digested with BstNI plus BglI, a novel anomalous DNA fragment 49% of the length of mature DN[r] ...
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DNA packaging in vitro by an isolated bacteriophage T7 procapsid.
... It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts de[r] ...
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Purification and structures of recombining and replicating bacteriophage T7 DNA.
... This structure was seen mn at 15 in a sample of EcoRI treated intracellular DNA taken after infection and sedimenting faster than unit-sized phage DNA.. The region at c is therefore undo[r] ...
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Role of gene 2 in bacteriophage T7 DNA synthesis.
... It was observed that after infection with T7 wild type a major portion of the newly synthesized DNA sedimented in both alkaline and neutral sucrose gradients 1.3 to 1.6 times as fast as [r] ...
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Accumulation of bacteriophage T7 head-related particles in an Escherichia coli mutant.
... In this paper, we describe the results of sedimentation analysis of lysates from T7-infected Y49 cells which indicate that the short DNA molecules are not packaged into phage heads, and [r] ...
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Derivation of a restriction map of bacteriophage T3 DNA and comparison with the map of bacteriophage T7 DNA.
... Hindul egi 177 KPnI XbaI AaI MboI HpaI 40 A- A- A- ~ A~ A RESULTS AThe restriction patterns generated by cleavage B of T3 DNA with the seven enzymes considered in this paper are shown in[r] ...
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Bacteriophage T7 DNA Synthesis in Isolated DNA-Membrane Complexes
... DNAmembrane complexes from T7 infected cells were isolated and incubated in the standard reaction mixture, and the DNA was extracted as described in Methods.. Repair of the isolated DNA [r] ...
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Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA
... The positions of promoter and terminator sites for the T7-specific RNA polymerase were estimated from the molecular weights of transcripts from exonucleolytically shortened templates 7; [r] ...
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Chemical stability of bacteriophage T7 early mRNA.
... At 20 min after T7 infection 12 min after rifampin addition, at which time total T7 early mRNA has lost messenger activity, individual mRNA species still remain as large size RNAs Fig.. [r] ...
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In vitro recombination of bacteriophage T7 DNA damaged by UV radiation.
... DNA from T7%8 phage was irradiated and incubated with extracts from T73- or T73,6-infected strain W3110 under conditions that would permit both replication and recombination.. Controls n[r] ...
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Electron microscopic analysis of partially replicated bacteriophage T7 DNA.
... 45[Suppl.]:51-74, 1955 as an explanation for the distribution of marker rescue frequencies during cross-reactivation; i.e., replication proceeds in a bidirectional manner from an origin [r] ...
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Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.
... This is particularly true of RNA synthesis, where only late transcription appears to be inhibited by coumermycin; but with DNA synthesis, which is more completely inhibited by the antibi[r] ...
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Effect of RNase III on the size of bacteriophage T7 lysozyme mRNA.
... Analysis by preparative gel electrophoresis of RNA extracted after infection of Escherichia coli B8t RNase III' separated lysozyme mRNA into six peaks of activity ranging in size from 0.[r] ...
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Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.
... More recently, the nonionic detergent Brij-58 was shown to produce a similarly fast-sedimenting structure, which in electron micrographs appeared to consist of up to 30 phage equivalents[r] ...
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Revised transcription map of the late region of bacteriophage T7 DNA.
... Through the use of an agarose-formaldehyde gel system that permits the resolution of high-molecular-weight RNAs, transcription of template DNA that had been cut with various restriction [r] ...
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Association of Replicating Bacteriophage T7 DNA with Bacterial Membranes
... The major portion of the newly synthesized T7 DNA cosedimented Control Pronase Trypsin DNase RNase Phospholipase A Percent of control exhibiting tast sedimentation rate Membranes DNA 10 [r] ...
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Physical Mapping of Hybrid Bacteriophage T7/T3 RNA Polymerase Genes
... There were no new fragments in In summary, the HpaI data provide physical the Rb37 HpaI digest; there was a new fragment evidence that crossovers have occurred in the of 6,000 base pairs[r] ...
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