Baculovirus expression system
The effect of time and optimization of the baculovirus expression system for more efficient recombinant protein production of porcine epidemic diarrhea virus in insect cells
... The baculovirus expression vector system (BEVS) has many strengths, such as the capacity for large inserts of DNA and a high yield of recombinant ...titer baculovirus stock; (ii) determination ...
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Expression of Sendai virus nucleocapsid protein in a baculovirus expression system and application to diagnostic assays for Sendai virus infection
... the baculovirus expression system was tested as an approach to improving the specificities of ELISAs for the detection of antibodies to Sen- dai virus and eliminating the tedious procedures ...
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Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... eukaryotic expression systems have been used for in vitro production of the subunit vaccines from foreign ...Eukaryotic expression system is the first choice for production of proteins that undergo ...
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Designing a recombinant Bacmid construct of HCV core+1 in Baculovirus expression system
... Background and Objectives: Hepatitis C virus (HCV) chronically infects around 200 million people worldwide and fre- quently causes liver cirrhosis and hepatocellular carcinoma. Rapid detection of this virus results in ...
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Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system
... these expression systems, the insect cell- based expression system has been widely used for VLP production (Noad and Roy 2003; Yamaji 2014; Fernandes et ...
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Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system.
... Western blot analysis B and Coomassie blue staining A of proteins from lysates of 3 x 106 SF9 cells in 100-mm tissue culture dishes mock-infected lanes 2 or infected with wild-type AcMNP[r] ...
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Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system.
... We report the successful production of the major capsid antigen VP6 of the simian rotavirus SAl1 in insect cells, using the baculovirus Autographa californica nuclear polyhedrosis virus [r] ...
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Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
... ent expression systems, including bacterial, yeast, insect, mammalian, and plant systems ...the baculovirus expression vector system (BEVS) [11], which provides a rapid and efficient method to ...
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Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... type baculovirus control, VP4 expression level to native polyhydrin protein (29 kDa) could not be ...VP6 expression in Sf9 cells by Estes ...The baculovirus expressed VP4 protein may be useful ...
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Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
... by expression of the HCV structural pro- teins in the baculovirus expression system (3) or by Semliki Forest virus replicons ...the baculovirus and Semliki Forest virus expres- sion ...
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Structural Requirements of Astrovirus Virus-Like Particles Assembled in Insect Cells
... The baculovirus expression system has extensively been used to express capsid protein virus genes and to produce self- assembled VLPs which represent a good tool for the study of viral morphogenesis ...
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Expression and purification of envelop capsid proteins of foot and mouth disease virus type o isolated in vietnamin baculovirus system for making virus like particle
... The researches have strongly demonstrated the effect of VLP- based vaccine in against to B-cell and T-cell antigens. However, during the infection, many pathogens expose antigenic variation in response to host immune ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... coli expression vector pGEMEX-2 (Promega ...Bac-to-Bac baculovirus expression system (Life Technolo- ...The expression of the RAP-1 gene was confirmed by IFAT and Western blotting ...
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Identification and characterisation of the interaction between Wiskott-Aldrich syndrome protein (WASP) and c-Src kinases
... I initially was unable to co-immunoprecipitate endogenous WASP from cell extracts, with any of its potential binding partners. A variety of cell lines, subjected to different stimuli were used, together with various ...
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Characterization of baculovirus-expressed herpes simplex virus type 1 glycoprotein K.
... We report here for the first time that i HSV-1 gK was expressed in a baculovirus expression system; ii the expressed gK was detected in a partially glycosylated precursor pgK form and a [r] ...
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A baculovirus dual expression system based vaccine confers complete protection against lethal challenge with H9N2 avian influenza virus in mice
... the baculovirus can directly transduce APCs [24,25] resident in the muscle tissues, especially more efficient antigen presentation to dendritic cells (DCs), which are the most important APCs ...recombinant ...
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Differentiation of Classical Swine Fever Virus Infection from CP7_E2alf Marker Vaccination by a Multiplex Microsphere Immunoassay
... bodies by ELISA is problematic, which is likely due to the cross- reactivity with antibodies against BVDV or BDV). Within the framework of the EU project “CSFV_goDIVA,” two CSFV E rns - based ELISAs were developed using ...
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Autographa californica Nucleopolyhedrovirus orf69 Encodes an RNA Cap (Nucleoside-2′-O)-Methyltransferase
... Primer extension assays mapped mtase1 transcripts to an ATAAG motif, which is common to baculovirus late gene promoters. A late transcription start site was also mapped to a noncanonical site. The presence of this ...
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Synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay.
... Abundant synthesis of functional human T-cell leukemia virus type I p4Y protein in eucaryotic cells by using a baculovirus expression vector.. A baculovirus vector can express intron-con[r] ...
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