Basophil activation
The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test
... with basophil activation and gating strategies that depend solely on this marker lead to the loss-to-analysis of activated basophils and to the underestimation of basophil ...following ...
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Basophil activation test based on the expression of CD203c in the diagnostics of cow milk allergy in children
... a basophil activation test in pre- dicting the child's reaction to the oral challenge, evalu- ating and comparing the specific ige and skin prick test ...sophil activation based on Cd63-upregulation ...
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Threshold for basophil activation test positivity in neuromuscular blocking agents hypersensitivity reactions
... of basophil activation by flow cytome- try (the basophil activation test, BAT) has been proven to be a useful tool for the assessment of the immediate-type response to allergens mediated by ...
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The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives
... to basophil functional studies was the hista- mine release ...ing basophil activation upon allergen challenge by detecting surface expression of degranulation markers ...
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Differential response of human basophil activation markers: a multi-parameter flow cytometry approach
... After basophil treatment with fMLP and with anti-IgE, an increase in MFI of CD63, CD203c, CD45 and CD13 was observed (the latter only with fMLP), while CD69, used by others as activation marker ...
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The diagnostic usefulness of the basophil activation test (BAT) with annexin V in an allergy to Alternaria alternata
... analyze basophil activation, cells were stained with 5 µL of annexin V-FITC (BD Biosciences) and 5 µL of anti-CCR3-PE (R&D Systems, Minneapolis, USA), and the samples were incubated for 15 min at ...
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Use of a basophil activation test as a complementary diagnostic tool in the diagnosis of severe peanut allergy in adults
... Basophil activation was measured based on the up- regulation of CD63 on CD203c + basophils observed in flow cytometry of blood samples collected in heparinized ...the activation of basophils, the ...
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The utility of the basophil activation test in the diagnosis of immediate amoxicillin or amoxicillin-clavulanate hypersensitivity in children and adults
... the basophil activation test (BAT) has been proposed as a possible functional assay for the diagnosis of allergy to ...different activation markers, such as CD63 and ...
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Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E
... Figure 2 Basophil activation assay performed (1) with or (2) without IgE staining. Buffy coat cells suspended in an IL-3-containing buffer were (A) stimulated with birch allergen or (B) not stimulated ...
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Evaluation of basophil activation caused by transgenic rice seeds expressing whole T cell epitopes of the major Japanese cedar pollen allergens
... The basophil activation test (BAT) has been applied to investigate IgE-mediated allergy caused by classical inhalant allergens, food, Hevea latex, hymenoptera ven- oms, and drugs [23, ...
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Basophil activation test: food challenge in a test tube or specialist research tool?
... decreased basophil reactivity to the respective food allergens with treatment, which is particularly evident at the lower concentrations of the allergen, reflecting the decrease in basophil sensi- ...in ...
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Diagnosis of Vespa affinis venom allergy: use of immunochemical methods and a passive basophil activation test
... as activation marker [40–42, 55, ...the basophil activation marker (CD203c) that was used, could be upregulated and expressed in response to many non-degranulation stimuli (piecemeal ...
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Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI
... The basophil activation test (BAT), in which an allergen- specific response is measured by flow cytometry (reviewed in Ebo et al [1]), is gaining popularity as an ex vivo diag- nostic ...
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Aged mice display altered numbers and phenotype of basophils, and bone marrow-derived basophil activation, with a limited role for aging-associated microbiota
... higher than 200%. Our purification method, which in- cludes dendritic cell removal, resulted in higher numbers of pure basophils: we isolated on average 6.9 × 10 6 pure basophils per 20 × 10 6 cultured cells (35% ...
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Anaphylactic Shock Due to Allergy to Macrogol 4000 Contained in SonoVue®
... The allergy study, summarized in Table 1, included: 1) Skin tests with Sono- Vue® (as previously described [2]), different molecular mass macrogols and Tween 80, and 2) Basophil activation test (BAT) with ...
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Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy
... The present data gave us an opportunity to determine the optimal allergen concentration for diagnosing wasp allergy with a ROC curve to be 0,1 ug/ml final concen- tration in the reaction vessel with blood, which com- ...
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Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals
... of basophil surface markers ...a basophil-specific marker and has been shown to be less specific and less sensitive (in grass pollen and house dust mite allergy, latex allergy, wasp venom hypersensitivity, ...
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Guidelines for the use and interpretation of diagnostic methods in adult food allergy
... AMA: allergen microarray; APT: atopy patch test; BAT: basophil activation test; DBPCT: double‑blind placebo‑controlled test; FA: food allergy; FDEIA: food dependent exercise induced an[r] ...
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Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses
... the activation of human basophil cells derived from birch/grass pollen- and house dust mite-allergic patients in response to free allergen and AuNP-allergen conjugates were determined using the ...
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Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets
... in basophil activation, such as calcium, protein kinase C (PKC) and ...In basophil biology a first step to be forwarded could be investigating the differential pattern between anaphylactic ...
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