BeWo cells
Effect of Silencing SLPI Gene Expression on Differentiation of BeWo Cells
... in BeWo cells have been able to identify several genes which have been grouped in to four categories based on their functional mportance like cell proliferation/differentiation, apoptosis, cell migration, ...
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Inhibition of Akt activity and calcium channel function coordinately drive cell cell fusion in the BeWO choriocarcinoma placental cell line
... on BeWo fusion are a result of PI3K inhibition in combination with the inhibition of the calcium channel ...1. BeWo and JEG cells expressing dsRED and GFP are functionally ...The cells were ...
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Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation
... line BeWo provides another in vitro model of cytotrophoblast ...levels), BeWo cells fuse to form a syncytium, a phenomenon that mimics the fusion of cytotrophoblast cells to form syncy- ...in ...
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In vitro placental model optimization for nanoparticle transport studies
... PS. BeWo cells grown on PC Transwell membranes (3 µ m pores) were fixed in 2% (v/v) PFA at 4 ° C for 1 hour, washed in phosphate-buffered saline (PBS) and permeabilized with ...temperature. Cells ...
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11ß HSD2 dependent and independent signalling pathways in trophoblast biology and glucocorticoid responsiveness
... that BeWo cell fusion and differentiation can be successfully established and monitored upon forskolin stimulation (Figure ...2011]. BeWo cells morphologically fuse after forskolin treatment which is ...
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Original Article Effect of Bisphenol A on invasion ability of human trophoblastic cell line BeWo
... Many researches focused on the endometrial receptivity during implantation. It was suggest- ed that BPA exposure could decrease the num- ber of implantation sites and the expression of endometrial receptivity associated ...
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Proteasome independent degradation of HIV 1 in naturally non permissive human placental trophoblast cells
... proteasome-inhibited cells for the R5 and X4 HIV-1 pseudotypes was only seen at the first time point measurements following ...treated cells and untreated cells was no longer ...proteasome-inhibited ...
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Original Article Mechanism underlying the regulation of trophoblast-like cell invasion by miR-183 screened from the peripheral blood of pregnant women with preeclampsia
... In order to identify the poten- tial targets of miR-183, we carried out an unbiased computational screening us- ing TargetScan, and identi- fied MMP9 as a potential tar- get of miR-183 (Figure 3A). To test whether MMP9 ...
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Hepatitis B Virus Translocates across a Trophoblastic Barrier
... blue. BeWo cells form a confluent, tight, polarized mono- ...show BeWo nuclei in a single layer. (B) In un- treated BeWo cells (No Drug), intracellular DHBV is in vesicular ...
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Characterization of VAR2CSA deficient Plasmodium falciparum infected erythrocytes selected for adhesion to the BeWo placental cell line
... to BeWo cells, in experiments similar to those reported here ...to BeWO was partly blocked by ...on BeWo were not recognized in a par- ity-dependent manner by immune sera, whereas one of our ...
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Organs-on-chip models of the female reproductive system
... trophoblast cells and human placental villous endothelial cells under dynamic flow ...endothelial cells (HPVECs) isolated from term placentas and BeWo b30 human trophoblast cell line have been ...
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Regulation of pregnancy-associated plasma protein A2 (PAPPA2) in a human placental trophoblast cell line (BeWo)
... We thank Gordon Rintoul, Audrey Speelman, Bryce Pasqualotto, and Tim Beischlag for technical support and the use of their equipment, Dan Rurak and Gratien Prefontaine for providing input into study design, and Andrée ...
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Distinct efficacy of HIV 1 entry inhibitors to prevent cell to cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro
... labelling, cells were washed extensively with PBS and used as effector ...effector cells (HIV-1 infected PBMCs) and target cells (BeWo cells), the latter were equilibrated in RPMI1640 ...
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Induction of pro inflammatory response of the placental trophoblast by Plasmodium falciparum infected erythrocytes and TNF
... the BeWo cells, characterized by an increase in the mICAM-1 expression, sICAM-1 release, and secretion of pro-inflammatory cytokines, such as IL-8 and ...
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Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model
... vitro BeWo cell model to determine the nontoxic concentrations of fluorescent polystyrene ...across BeWo cells grown on polycarbonate membranes ...across BeWo cell layers compared with free ...
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Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells
... experiments, BeWo cells were plated in 24-well dishes at 1×10 5 cells/well and cultured for 24 h in medium without ...antibiotics. Cells were transfected with siRNAs (5 nM/well) using a ...
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Role of mammalian target of rapamycin (mTOR) signalling in BeWo trophoblast differentiation and fusion
... of BeWo cells with forskolin almost eliminates mTOR phosphorylation at Ser2448 and ...for BeWo cells ...of BeWo cells results in down regulation of mTOR mRNA expression and its ...
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Effects of corticotrophin releasing hormone (CRH) on cell viability and differentiation in the human BeWo choriocarcinoma cell line: a potential syncytialisation inducer distinct from cyclic adenosine monophosphate (cAMP)
... in BeWo cells suggests that CRH could be involved in another bioactivity, such as modulation of differentiation or syncytialisation of tropho- blast ...trophoblast cells, the widely accepted marker ...
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Original Article Adiponectin exerts antiproliferative effect on human placenta via modulation of the JNK/c-Jun pathway
... of BeWo cell proliferation was mediated by the JNK/c-Jun ...of BeWo cells to levels comparable to those in controls (Figure ...of BeWo cells, we knocked-down c-Jun by tran- sient ...
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Increased resistance to apoptosis during differentiation and syncytialization of BeWo choriocarcinoma cells
... forskolin-treated BeWo chorio- carcinoma cells, a model for villous cytotrophoblast ...differentiation. BeWo cells were treated with forsko- lin or vehicle alone for up to 72 h and evaluated ...
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