Bimolecular fluorescence complementation
Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
... protein complementation assays exist that would lend themselves also to the establishment of high- throughput assays besides split-luciferase, for example dihy- drofolate reductase (DHFR), split-ubiquitin and ...
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Distinct Intracellular Trafficking of Equine Infectious Anemia Virus and Human Immunodeficiency Virus Type 1 Gag during Viral Assembly and Budding Revealed by Bimolecular Fluorescence Complementation Assays
... used bimolecular fluorescence complementation to charac- terize and compare assembly sites and budding efficiencies of EIAV and HIV-1 Gag in both human and rodent ...
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Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells
... function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as a relatively easy and inexpensive method to visualize protein-protein interactions in living ...
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Use of Bimolecular Fluorescence Complementation To Study In Vivo Interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae
... Saccharomyces cerevisiae Cdc42p functions as a GTPase molecular switch, activating multiple signaling pathways required to regulate cell cycle progression and the actin cytoskeleton. Regulatory proteins control its GTP ...
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Detection and Characterization of Influenza A Virus PA-PB2 Interaction through a Bimolecular Fluorescence Complementation Assay
... ing fluorescence imaging data of cells transfected with PA- GFP/PB2 to those of cells transfected with PA-GFP alone, as shown in that earlier report (8), we noticed that the expression of PB2 triggered some ...
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Identification of Transmembrane Protein 134 as a Novel LMP1-Binding Protein by Using Bimolecular Fluorescence Complementation and an Enhanced Retroviral Mutagen
... Fluorescence and confocal microscopy. Cells were plated on cover- slips and fixed 48 h postselection for retrovirus infection or 24 h post-dox induction with 4% paraformaldehyde in PBS for 10 min at 4°C and washed ...
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MCM GINS and MCM MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast
... lar fluorescence complementation (BiFC) to this problem, for the first time using this method in the unicellular fis- sion yeast ...of fluorescence energy resonance trans- fer (FRET) techniques, all ...
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Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
... the complementation between split Venus fragments, living embryos were dechorio- nated and mounted in the halocarbon oil 10S (commer- cialized by VWR, Pennsylvania, ...dermal fluorescence were taken in ...
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Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
... One of the inherent challenges for FCS and PCH analysis is to cor- relate changes in observed parameters, such as particle diffusion co- ef fi cients or brightness, with clearly de fi ned signalling events and known ...
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Characterization of the latent membrane protein 1 signaling complex of Epstein Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation
... BiFC with the LMP1 cytoplasmic domain and TRAFs Binding between LMP1 and the TRAFs was previously identified using the cytoplasmic domain of LMP1 in yeast two-hybrid (Y2H) screens [48]. To determine if LMP1 + TRAF2 or ...
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Bimolecular Complementation of Paramyxovirus Fusion and Hemagglutinin-Neuraminidase Proteins Enhances Fusion: Implications for the Mechanism of Fusion Triggering
... by bimolecular fluorescence complementation (BiFC), the cytoplasmic tails of parainfluenza virus 5 (PIV5) F and HN were fused to complementary fragments of yellow fluorescent protein ...in ...
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The Tobacco mosaic virus Movement Protein Associates with but Does Not Integrate into Biological Membranes
... Taking these results together with the behavior of the protein in response to different chemical treatments, the results of bimolecular fluorescence complementation BiFC studies, and the[r] ...
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A Physical Interaction between Viral Replicase and Capsid Protein Is Required for Genome-Packaging Specificity in an RNA Virus
... system. Bimolecular fluorescence complementation in conjunction with fluorescent cellular protein markers and coimmunoprecipita- tion assays demonstrated that FHV replicase (protein A) and CP ...
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Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1 viral protein R dimerization
... a bimolecular fluorescence complementation (BiFC)-based screening assay was developed that can quantify changes in dimerization, using HIV-1 viral protein R (Vpr) dimerization as a “proof of ...and ...
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Computational Modelling Folate Metabolism and DNA Methylation: Implications for Understanding Health and Ageing
... A deterministic ODE based kinetic model constructed using the assumption that most reactions in the folate cycle are bimolecular. Reed et al[r] ...
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Bimolecular Complementation Defines Functional Regions of Herpes Simplex Virus gB That Are Involved with gH/gL as a Necessary Step Leading to Cell Fusion
... When HSV receptor-bearing cells, such as B78H1 cells that are engineered to express nectin-1, are transfected with plas- mids that express gB, gD, gH, and gL, they undergo cell-cell fusion (13, 15, 27, 31, 48). When gD ...
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Proton Impact of Bimolecular Targets
... In the present work the dielectric formalism like Drude has been used to calculate the probability for an energetic proton and to produce electronic excitations in five targets of[r] ...
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18) Spectroscopic and modeling analyses of bimolecular structure of corn silk
... Quantitative structure activity relationship (QSAR) was calculated for the same structure using SCIGRESS 3.0 software [33].. Corn silk powder was subjected to FTIR spectroscopic [r] ...
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CYTOGENETIC ANALYSIS OF THE CHROMOSOMAL REGION IMMEDIATELY ADJACENT TO THE ROSY LOCUS IN DROSOPHILA MELANOGASTER
... On the basis of its localization by complementation with rosy-region deficiencies, this complementation group corresponds to the previously described Z(3)Sd locus ( SCHALET[r] ...
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GENETIC CHARACTERIZATION OF THE 87C REGION OF THE THIRD CHROMOSOME OF DROSOPHILA MELANOGASTER
... Mutations within a single complementation group have similar lethal phases or subvital phenotypes, consistent with the notion that each complementation group represents[r] ...
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