Bimolecular fluorescence complementation (BiFC)
Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells
... function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as a relatively easy and inexpensive method to visualize protein-protein interactions in living ...cells. ...
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Use of Bimolecular Fluorescence Complementation To Study In Vivo Interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae
... of bimolecular fluorescence complementation (BiFC) was used to study the dynamic in vivo inter- actions between Cdc42p and ...The BiFC data indicated that Cdc42p and Rdi1p interacted in ...
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Distinct Intracellular Trafficking of Equine Infectious Anemia Virus and Human Immunodeficiency Virus Type 1 Gag during Viral Assembly and Budding Revealed by Bimolecular Fluorescence Complementation Assays
... used bimolecular fluorescence complementation to charac- terize and compare assembly sites and budding efficiencies of EIAV and HIV-1 Gag in both human and rodent ...
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Detection and Characterization of Influenza A Virus PA-PB2 Interaction through a Bimolecular Fluorescence Complementation Assay
... using BiFC, we observed an interaction between PA and PB2 proteins that has not been reported previously, although we cannot rule out that the interaction of PA with PB2 is indirect and mediated by the cellular ...
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MCM GINS and MCM MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast
... lar fluorescence complementation (BiFC) to this problem, for the first time using this method in the unicellular fis- sion yeast ...pombe. BiFC offers a powerful method for studying ...
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Identification of Transmembrane Protein 134 as a Novel LMP1-Binding Protein by Using Bimolecular Fluorescence Complementation and an Enhanced Retroviral Mutagen
... by fluorescence micros- ...for BiFC by fluorescence ...background fluorescence. VC1-clone 1 was the only clone which exhibited BiFC following induction with dox ...perinuclear ...
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Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
... Unravelling the stoichiometry of receptor interaction with effectors, such as G proteins or β -arrestins, is a key challenge to advancing our un- derstanding of GPCR pharmacology [55,56]. For β -arrestins a theoretical ...
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Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
... positive BiFC cells sorted by FACS did lead to a slight enrichment of the BiFC generating plasmids (Figure 4E), other plasmids were still present in those protoplasts and also consequently in the ...on ...
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Characterization of the latent membrane protein 1 signaling complex of Epstein Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation
... induces fluorescence complementation, BiFC assays were ...into BiFC expression plasmids as fusion proteins with the amino-terminus of YFP (NYFP) or the carboxyl-terminus of YFP ...
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Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
... for BiFC analyses were set up at the defined temperature over ...the complementation between split Venus fragments, living embryos were dechorio- nated and mounted in the halocarbon oil 10S (commer- ...
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Bimolecular Complementation of Paramyxovirus Fusion and Hemagglutinin-Neuraminidase Proteins Enhances Fusion: Implications for the Mechanism of Fusion Triggering
... by bimolecular fluorescence complementation (BiFC), the cytoplasmic tails of parainfluenza virus 5 (PIV5) F and HN were fused to complementary fragments of yellow fluorescent protein ...the ...
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Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1 viral protein R dimerization
... a bimolecular fluorescence complementation (BiFC)-based screening assay was developed that can quantify changes in dimerization, using HIV-1 viral protein R (Vpr) dimerization as a “proof of ...
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A Physical Interaction between Viral Replicase and Capsid Protein Is Required for Genome-Packaging Specificity in an RNA Virus
... system. Bimolecular fluorescence complementation in conjunction with fluorescent cellular protein markers and coimmunoprecipita- tion assays demonstrated that FHV replicase (protein A) and CP ...
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Bimolecular Complementation Defines Functional Regions of Herpes Simplex Virus gB That Are Involved with gH/gL as a Necessary Step Leading to Cell Fusion
... When HSV receptor-bearing cells, such as B78H1 cells that are engineered to express nectin-1, are transfected with plas- mids that express gB, gD, gH, and gL, they undergo cell-cell fusion (13, 15, 27, 31, 48). When gD ...
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Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System
... the bimolecular fluorescence complementation (BiFC) ...including BiFC, were frequently used to reveal the dynamics of PPIs in vivo and in vitro (20, 22, ...
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Regulation of Latent Membrane Protein 1 Signaling through Interaction with Cytoskeletal Proteins
... by fluorescence-acti- vated cell sorting (FACS), and single-cell clones were examined for the targeted open reading ...(Tpm). BiFC between LMP1 and the cytoskeletal proteins was decreased by LMP1 signaling ...
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Kinetic study of solvolysis of iso amyl iodide in alkaline and neutral media
... Effect of solvent variation on the rate of bimolecular substitution and elimination reactions From perusal of table 7 it is clear that by decreasing the percentage of alcohol, the veloci[r] ...
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Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles
... Complementation is essential to unequivocally confirm the role of a genetic determinant in a specific phenotype [30]. Comple- mentation is traditionally achieved using multicopy plasmids. The consequent abnormal ...
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Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation
... after complementation of this mutation in cis ...in complementation derivative was lower than in the wild type strain, which was not unexpected as full restoration of activity requires fine tuning of gene ...
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Computational Modelling Folate Metabolism and DNA Methylation: Implications for Understanding Health and Ageing
... A deterministic ODE based kinetic model constructed using the assumption that most reactions in the folate cycle are bimolecular. Reed et al[r] ...
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