Blotting analysis
Immunoenzyme Western blotting analysis of antibody specificity in Aleutian disease of mink, a parvovirus infection.
... The sera of 27 pastel non-Aleutian and 15 sapphire Aleutian mink infected for various times with ADV Pullman strain, a low-virulence strain, were tested by blotting analysis, and represe[r] ...
5
Cell penetrating caspase substrates promote survival of the transplanted cells
... Western Blotting analysis of the lysates from neuronal N1E cells exposed to 100 nM Staurosporine in the presence of the two pep- tides at the above described conditions ...
6
Gankyrin sustains PI3K/GSK-3β/β-catenin signal activation and promotes colorectal cancer aggressiveness and progression
... (IHC) analysis was conducted to study Gankyrin protein expression in 217 human paraffin-embedded CRC ...western blotting analysis showed that Gankyrin protein expression was significantly increased ...
16
Class II phosphoinositide 3-kinase C2β regulates a novel signaling pathway involved in breast cancer progression
... Densitometry analysis indicated that downregulation of PI3K-C2β selectively affected phosphorylation/activation of specific proteins (Figure 4B, ...Western blotting analysis to validate some of the ...
21
Promoter methylation and downregulation of SLC22A18 are associated with the development and progression of human glioma
... RT-PCR analysis indicated that SLC22A18 mRNA was significantly decreased or absent in all of the 15 gliomas in which the SLC22A18 promoter was methylated, compared to adjacent normal brain tissues (Figure ...
14
Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-β
... Blockage of HMGB1 inhibited PEDV infection It is well-known that HMGB1 released into the extracellular space can elicit potent inflammatory responses [21]. The above experiments revealed that HMGB1 was actively released ...
17
FoxO3a confers cetuximab resistance in RAS wild-type metastatic colorectal cancer through c-Myc
... 5D).Western blotting analysis of CRC cell lines showed that expression of c-Myc proteins also elevated in HT29 and colo205 cells (Figure 5E) as well as in Caco2-CR cells (Figure ...
13
Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion
... western blotting analysis confirmed that a high level of ARHGDIA protein expression was achieved in infected cells compared with the negative control-infected cells (Figure ...
16
Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in H9c2 cardiomyoblasts
... western blotting analysis showed that LPS treatment stimulated COX-2 expression, but that this LPS-induced expression was significantly downregulated by luteolin, genistein and ...
12
PRODUCTION OF POLYCLONAL ANTIBODIES SPECIFIC TO CITRUS TRISTEZA VIRUS (CTV) USING THE 6X HIS-TAGGED FUSION COAT PROTEIN
... Citrus tristeza virus (CTV) is one of the most destructive and economically important diseases of commercial citrus worldwide. In this study, the coat protein (cp) gene of CTV was subcloned into PQE-30 vector at Bam HI ...
6
Molecular cloning, expression and characterization of a novel geneβ N acetylglucosaminidase from Bombyxmori
... 0.5, the expression of BmGlcNAcase2 was induced with IPTG (final concentration 0.1-1 mM during optimization) and further cultured at 37˚C for another 8 hours and 16˚C for another 12 hours respectively. Cells were har- ...
5
The Cellular RNA Helicase DDX1 Interacts with Coronavirus Nonstructural Protein 14 and Enhances Viral Replication
... Western blotting of cells expressing the HA-tagged SARS-CoV nsp14 protein either on its own or together with the Flag-tagged DDX1 protein, using anti-HA monoclonal antibody, detected the protein ...Western ...
13
Hyperhomocysteinemia Exacerbates Cisplatin-induced Acute Kidney Injury
... Renal ischemia-reperfusion was induced in mice as described previously [21,22]. Briefly, mice were kept on a homeothermic station to maintain body temperature at 37°C. Kidneys were exposed by bilateral flank incisions, ...
13
A Putative Rhamnogalacturonase Required for Sexual Development of Neurospora crassa
... Western blot analysis Western blotting was per- formed to characterize the ASD-1 polyclonal antibodies (raised to a potentially antigenic peptide of the ASD-1 protein) and [r] ...
10
3Cpro of Foot-and-Mouth Disease Virus Antagonizes the Interferon Signaling Pathway by Blocking STAT1/STAT2 Nuclear Translocation
... pXJ41-GST, pXJ41-NS1, pXJ41-3ABC, pXJ41-3A, pXJ41-3B, or pXJ41-3C and 0.5 g of pISRE-Luc along with 0.05 g of pRL-TK as an internal control. pXJ41-GST and pXJ41-NS1 were used as negative and positive controls, ...
13
Patients with rheumatoid arthritis have an altered circulatory aggrecan profile
... Measurement of total aggrecan turnover, G1/G2, in human OA patient. Human cartilage explants were cul- tured for 21 days either non-stimulated (-t-), or treated with pro-inflammatory cytokines 10 ng/ml oncostatin M (OSM) ...
9
Analysis of the Echinococcus granulosus Laminated Layer Carbohydrates by Lectin Blotting
... Cystic hydatid disease is caused by cystic larval (metacestode) stages of Echinococcus granulosus. This parasite can potentially occur all over the world especially in Mediterranean and Middle East countries and some ...
5
A cord blood monocyte–derived cell therapy product accelerates brain remyelination
... Microarray analysis, quantitative PCR studies, Western blotting, and flow cytometry demonstrated that expression of factors that promote remyelination including PDGF-AA, stem cell factor, IGF1, MMP9, MMP12, ...
20
Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania
... blot analysis was carried out by using the purified protein as an antigen and the T7-Tag monoclonal antibody or anti- Leishmania major PTR1 [18] as the primary ...
6
Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury
... adenovirus shuttle plasmid vector, which contains a pro- moter of the human phosphoglycerate kinase (PGK) and a polyadenylation signal of bovine growth hormone [22]. Adenoviral administration was followed the procedure ...
16