Bone-marrow derived dendritic cells
Comparison of immature and mature bone marrow derived dendritic cells by atomic force microscopy
... Bone marrow-derived dendritic cells were generated according to Lutz ’ s publication [10] with a little modifica- ...the marrow was washed with PBS through a syringe. Clusters ...
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Original Article High-dose hepatitis B E antigen drives mouse bone marrow-derived dendritic cells to differentiate into regulatory dendritic cells in vitro
... Abstract: Dendritic cells (DCs) from patients with chronic hepatitis B virus (HBV) infection are functionally deficient, giving rise to more tolerogenic rather than immunogenic responses, which may conduce ...
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Effects of histamine and its antagonists on murine T-cells and bone marrow-derived dendritic cells
... the cells were pulsed for 16 hours with OVa, OVa/lPs, OVa/hist, OVa/cim, or ...These cells were then cocultivated with cD4+-sorted cells and cD11c-depleted cells from ...the cells were ...
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Phenotypic and functional abnormalities of bone marrow derived dendritic cells in systemic lupus erythematosus
... To evaluate whether BM FLDCs comprise a subpopula- tion of pDCs and whether SLE BM FLDCs had higher pDC activity, we measured the level of IFN-α by using ELISA in the supernatants of BM FLDC and mDC cul- tures. IFN-α is ...
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Immunosuppressive effects of tick protein RHcyst 1 on murine bone marrow derived dendritic cells
... immune cells and is an important regulator of cell cytotoxicity ...T cells [9]. In human tissues and cells, cystatin C is the most abundant cystatin and may modulate the func- tion of different ...
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Immunosuppressive effects of Amblyomma cajennense tick saliva on murine bone marrow derived dendritic cells
... of cells at a concentration of 1×10 6 cells/ml was placed in the wells, with each well containing cells that were either untreated or pretreated with saliva for 18 hours at different concentrations ...
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Trichinella spiralis paramyosin activates mouse bone marrow derived dendritic cells and induces regulatory T cells
... Treg cells in vitro associated with higher level of IL-10 and TGF-β, the cytokines mostly secreted by Tregs ...Treg cells to modulate host immune response, possibly through signal passage from DC to T ...
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Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson’s disease
... Our current study was designed to assess the effects of GM-CSF and modified α-synuclein (α-Syn) on tolero- genic DCs and Treg induction. Herein, we show that GM-CSF-induced tolerogenic BMDCs lead to induction of Tregs ...
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Original Article Bortezomib inhibits bone marrow-derived dendritic cells
... and bone marrow (BM) cells were washed out using serum-free PRMI1640 ...blood cells by the buffer ...BM cells were cultured in PRMI1640 medium containing 10% FBS (GIBCO, US), 100 U/ml ...
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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
... Using EGFP as a reporter gene, DC were shown to be transfected more efficiently with DNA-loaded TATp-L compared to DNA-loaded plain-L. This correlates with the liposome uptake by DC and is especially important since the ...
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Modulation of Gene Expression Related to Toll-Like Receptor Signaling in Dendritic Cells by Poly(γ-Glutamic Acid) Nanoparticles
... murine bone marrow-derived dendritic cells (DCs) treated with ␥ -PGA NPs was examined by oligonucleotide microarray analysis and compared with that in cells treated with other ...
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Relief from Zmp1-Mediated Arrest of Phagosome Maturation Is Associated with Facilitated Presentation and Enhanced Immunogenicity of Mycobacterial Antigens
... murine bone marrow-derived dendritic cells with a mycobacterial mutant deficient in zinc metal- loprotease 1 (Zmp1) resulted in increased presentation of MHC class II-restricted ...
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Modified Fe3O4 Magnetic Nanoparticle Delivery of CpG Inhibits Tumor Growth and Spontaneous Pulmonary Metastases to Enhance Immunotherapy
... in bone marrow-derived dendritic cells (BMDCs) in vitro and through intratumoral injection, showed significant antitumor ability by stimulating better humoral and cellular immune ...
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A role for cathepsin Z in neuroinflammation provides mechanistic support for an epigenetic risk factor in multiple sclerosis
... 17]. Bone marrow-derived macrophages (BMMØs) were derived from bone marrow using L929-conditioned media and ac- tivated for 18 h with recombinant IFNγ (rIFNγ) (100 U/ ml, Pepro ...
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Bone Marrow Dendritic Cells from Mice with an Altered Microbiota Provide Interleukin 17A Dependent Protection against Entamoeba histolytica Colitis
... dritic cells, and neutrophils. Bone marrow-derived dendritic cells (BMDCs) from SFB-colonized mice had higher levels of IL-23 production in response to stimulation with ...on ...
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Exosomes derived from statin-modified bone marrow dendritic cells increase thymus-derived natural regulatory T cells in experimental autoimmune myasthenia gravis
... stromal cells were isolated as described pre- viously ...isolated cells were removed and replaced with the fresh mixture for further incuba- ...isolated cells were removed and collagenases/dispase ...
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Role of the IL-33-ST2 axis in sepsis
... BMDMs: Bone marrow-derived macrophages; CLP: Cecal ligation and puncture; DAMPs: Danger-associated molecular patterns; DCs: Dendritic cells; GMC- SF: Granulocyte macrophage ...
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IL-15 Generates IFN-γ-producing Cells Reciprocally Expressing Lymphoid-Myeloid Markers during Dendritic Cell Differentiation
... innate cells such as IKDCs or pre-mNK cells, NK cells, NKDCs and ILC1s, in the present study, we revisited the phenotypic characteristics of the IFN-γ-producing IL-15-differentiated bone ...
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Abnormalities in circulating plasmacytoid dendritic cells in patients with systemic lupus erythematosus
... pulsed bone marrow-derived DCs (AC-BMDCs) could induce the proliferation of self reactive T-cells resulting in tolerance break down and initiation of autoimmune responses in normal mice ...
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Autologous bone marrow cell therapy for peripheral arterial disease
... of bone marrow–derived stem and progenitor ...These cells participate in the development of new blood vessels, the enlargement of existing blood vessels, and sprouting new capillaries from ...
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