brain capillary endothelial cells
A large-scale electrophoresis- and chromatography-based determination of gene expression profiles in bovine brain capillary endothelial cells after the re-induction of blood-brain barrier properties
... the brain, only blood capillaries are endowed with a complete BBB phenotype ...unique endothelial features, including (i) the lack of fenestration, (ii) a decrease in the number of pinocytic vesicles, (iii) ...
11
Transfection of brain capillary endothelial cells in primary culture with defined blood–brain barrier properties
... Primary cultures of BCECs were prepared from 2 to 3 weeks old Sprague–Dawley rats using slight modifica- tions of the protocol of Nakagawa et al. [19]. The proce- dures dealing with the handling of rats as described in ...
14
Aloe-emodin triggers ROS and Ca 2+ production and decreases the levels of mitochondrial membrane potential of human brain capillary endothelial cells
... Immortalized brain capillary endothelial hCMEC/D3 cell line have been created from brain endothelial cells, isolated from human brain tissue via surgical excision of an ...
7
Cyclooxygenase-2 (COX-2) inhibitors: future therapeutic strategies for epilepsy management
... astrocytes, brain capillary endothelial cells, and circulating peripheral immune cells along with the production of in- flammatory mediators, initiated in response to a variety of ...
15
Immunohistochemical and in situ hybridization study of urate transporters GLUT9/URATv1, ABCG2, and URAT1 in the murine brain
... ependymal cells and neuronal somatic ...on brain capillary endothelial ...bEnd.3 cells (a murine brain endothelial cell line) [31, ...
10
Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses
... In most proteomics analyses and in particular for the “off-gel” approaches, based essentially on chromatography, the complexity of the proteome should be reduced; otherwise identifications can be hindered, especially if ...
6
Assessment of brain delivery of a model ABCB1/ABCG2 substrate in patients with non-contrast-enhancing brain tumors with positron emission tomography
... in brain tumors which may constitute promising targets for their treatment with molecularly targeted an- ticancer drugs ...the brain. The BBB consists of brain capillary endothelial ...
10
Intracerebral hemorrhages and syncytium formation induced by endothelial cell infection with a murine leukemia virus.
... Human immunodeficiency virus type 1 has been detected in brain capillary endothelial cells CEC of some AIDS patients 38, and it is postulated that retroviral infection of brain CEC may l[r] ...
10
In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain
... serum-free endothelial cell medium to dif- ferent concentrations (100–600 µ g/mL) and incubated with brain capillary endothelial cells, with cells not exposed to nanoparticles ...
11
"NEUROSURGICAL STRATEGY FOR DRUG DELIVERY TO BRAIN" by Jyotsna D. Chavan,* JohnI. D'souza, India.
... the brain. 3 The presence of tight junctions between the brain capillary endothelial cells means that the paracellular pathway for drug delivery is highly ...the brain ...
6
Studying Molecular Aspects of the Blood Brain Barrier Using an in Vitro Model: Contribution of a Global Proteomics Strategy
... and brain [1]. The morphology and functional properties of the brain capillary endothelial cells (BCEC) that form with other cells (Figure 1) the BBB are well documented: a ...
8
Drug Permeation across the Blood-Brain Barrier: Applications of Nanotechnology
... penetrate brain endothelial cells and deliver drugs to targeted sites of the central nervous system ...in brain capillary endothelial cells, and can be effectively used to ...
10
Flavonoid transport across RBE4 cells: A blood-brain barrier model
... RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this ...RBE-4 cells in a time-dependent ...
8
Characteristics of L-citrulline transport through blood-brain barrier in the brain capillary endothelial cell line (TR-BBB cells)
... Total RNA was isolated from cultured TR-BBB cells and rat cerebrum tissues by using the RNeasy Mini Kit from Qiagen (Qiagen, Valencia, CA) according the manufac- turer’s instructions. Total RNA (2 μg) was reverse- ...
10
Pathogenesis of Brain Edema and Hemorrhage: Role of the Brain Capillary
... It has recently been shown that the endothelial cells in brain capillaries are the anatomic site of the blood-brain barrier, and that these endothelial cells act to maintain a constant c[r] ...
6
Lipopolysaccharide-induced blood-brain barrier disruption: roles of cyclooxygenase, oxidative stress, neuroinflammation, and elements of the neurovascular unit
... The vascular blood-brain barrier (BBB) protects the central nervous system from blood-borne substances that are otherwise neurotoxic. Inflammation has long been known to disrupt the BBB. Skoog in 1937 found that ...
15
Immortalized Human Brain Endothelial Cell Line HCMEC/D3 as a Model of the Blood-Brain Barrier Facilitates In Vitro Studies of Central Nervous System Infection by Cryptococcus neoformans
... of brain endothelial cells in- fected with ...cryptococcal cells penetrated the HCMEC/D3 cell barrier, consistent with a trans- cellular movement of ...HCMEC/D3 cells had undergone ...
5
Brain vascular heterogeneity: implications for disease pathogenesis and design of in vitro blood–brain barrier models
... anesthetics, drugs of abuse, and barbiturates, dissolve into the cell membranes of CECs and diffuse across the BBB into the CNS. Other compounds can cross the BBB via more specific transcellular pathways which include: ...
12
15-deoxy-delta12,14-prostaglandin J2 attenuates endothelial-monocyte interaction: implication for inflammatory diseases
... h, cells were treated with 15d-PGJ 2 or trogliatzone in the presence or absence of GW9662 (10 μM) for 24 ...untreated cells; !!! p < ...treated cells. (C) Cells were trans- fected with ...
10
Diabetic Microangiopathy
... The nuclei of these endothelial cells protrude into the capillary lumen, but elsewhere the cytoplasm of these cells is spread out in a con- tinuous thin layer aro[r] ...
5