Bromodomain-containing protein 3
Trypanosoma cruzi Bromodomain Factor 3 Binds Acetylated α-Tubulin and Concentrates in the Flagellum during Metacyclogenesis
... recombinant protein were purified by affinity ...cytoplasmic bromodomain-containing protein in ...(NN) protein extracts (30 g per well) were subjected to Western blot analysis using ...
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The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila
... Fig. 6 Ibd1 is localized to actively transcribed genes. a Ibd1 occupancy. Ibd1 shows occupancy for 837 ORF and 396 intergenic regions. When Ibd1 was enriched (IP/INPUT) 2–3 (≥ 2), 4–5 (≥ 4), 6–8 (≥ 6) and more ...
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Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching
... encoding bromodomain-containing proteins fused to GFP (wild type or ...GFP-tagged protein due to saturation of potential binding sites and were, therefore, excluded from selection for ...different ...
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<p>microRNA-612 suppresses the malignant development of non-small-cell lung cancer by directly targeting bromodomain-containing protein 4</p>
... As miRNAs function by regulating the expression of their targets, 9 bioinformatics analysis was utilized to predict the potential target genes of miR-612. BRD4, which contains a complementary binding site for miR-612 ...
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A Bromodomain-Containing Host Protein Mediates the Nuclear Importation of a Satellite RNA of Cucumber Mosaic Virus
... another protein, 2b (that is expressed as a subgenomic RNA4A), and is the designated sup- pressor of posttranscriptional gene silencing (3, ...movement protein (MP) open reading frame (ORF) in the 5= ...
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Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
... Total RNA was extracted from the 40 paired tissue samples and from the cell lines (LO2, HepG2, Hep3B, Bel7402 and HCCLM6) using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. The ...
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The discovery of I-BRD9, a selective cell active chemical probe for bromodomain containing protein 9 inhibition
... BRD9 bromodomain would be invaluable in better understanding the biological role and therapeutic potential of this reader ...spectrum bromodomain inhibitor, which shows a thermal shift of ...mixed ...
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Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA DRB1 defined Canadian Yorkshire pigs
... member 3); BRD2, Bromodomain containing 2; CCL4, Chemokine (C-C motif) ligand 4; IL1B, Interleukin 1 beta; SLA-DQA, SLA class II DQ alpha; TLR2, Toll-like receptor 2; CASP1, Caspase 1; RPL19, ...
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Bromodomain protein 4 discriminates tissue-specific super-enhancers containing disease-specific susceptibility loci in prostate and breast cancer.
... association relative to that expected under the global null hypothesis [18]. Q-Q plots show that SNPs within regions with different genomic features (H3K27ac, BRD4, and MED12, or a combination of these) had dif- ferent ...
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RETRACTED ARTICLE: Molecular mechanisms in progression of HPV-associated cervical carcinogenesis
... serine/threonine-specific protein kinase; AP-1: Activator protein 1; Bcl- 2: B-cell lymphoma 2; BRD4: Bromodomain-containing protein 4; CADM1: Cell adhesion molecule 1; CARM1: ...
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Original Article Bromodomain 4 protein is a predictor of survival for urothelial carcinoma of bladder
... Paraffin sections (4 μm thick) were deparaffinized in xylene and rehydrated in grade alcohol, followed by boiling in 10 mmol/L of citrate buffer (pH 6.0) for antigen retrieval. After inhibition of endoge- nous peroxidase ...
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Proteasomal Degradation of the Papillomavirus E2 Protein Is Inhibited by Overexpression of Bromodomain-Containing Protein 4
... 3 ⬘ ; DBD (aa 266 to 372), 5 ⬘ -ATATAGGATCCTCCGTGGACAGTGTCAACT GTGG-3⬘ and 5⬘-TATATTCTAGATTAAATAGTCATATATCCTGTTGACA CTGATAC-3 ⬘ ; TAD ⫹ Hinge (aa 1 to 293), 5 ⬘ -TATATGGATCCGAGACTCT ...
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Influence of heat and pH on structure and conformation of whey proteins
... whey protein (WP) dispersions (7%, w/w, protein) in water (panel A; pH not adjusted) and at pH 3 in citric acid solution (panel B) or lactic acid solution (panel C) after heating at 140 °C for 30 ...
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Spatio-temporal regulation of EGFR signaling by the Eps15 homology domain-containing protein 3 (EHD3)
... To further analyze the role of EHD3 in ligand- dependent degradation of EGFR, we used the lysosome inhibitor Chloroquine. Pre-treatment with Chloroquine (25 μM, 30 min) in presence of CHX (10 μM), resulted in inhibition ...
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A Single Mutation in the Carboxy Terminus of Reovirus Outer-Capsid Protein σ3 Confers Enhanced Kinetics of σ3 Proteolysis, Resistance to Inhibitors of Viral Disassembly, and Alterations in σ3 Structure
... PI 3-1 virions and r3-1, rL/C, r2A1, and rA-C particles were ap- proximately 10- to 20-fold greater than those of wild-type T1L and T3D virions and rA, rB, and rA-B ...PI 3-1 virions and r3-1, rL/C, r2A1, ...
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Antimicrobial titanium/silver PVD coatings on titanium
... nium-alloys containing up to 9% silver by using the phys- ical vapour deposition (PVD) process and tested for hardness, biocompatibility and bactericide ...
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The BET bromodomain inhibitor exerts the most potent synergistic anticancer effects with quinone-containing compounds and anti- microtubule drugs
... genes. Protein co-IP, ChIP and luciferase assays show that Nrf2 forms protein complexes with BRD3 and BRD4, that treatment with nanaomycin increase Nrf2, BRD3 and BRD4 protein binding to the HMOX1 ...
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Cloning and expression of the constant region of rainbow trout (Onchorhynchus mykiss) µ immunoglobulin chain in Escherichia coli
... recombinant protein with an expected molecular weight of 75 ...recombinant protein was purified by amylose resin and its antigenicity was accessed by ...expressed protein with anti-trout serum ...
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Identification of a new viral protein containing CAp30 and NCp10 sequences in murine and feline leukemia retroviruses.
... These studies involved Western immunoblotting of virus and cell extracts with antibodies to CAp30 and NCp10. Cell and virus systems used were NIH 3T3 cells infected with a wild-type clone 1 Moloney murine leukemia virus ...
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Regulatory mechanisms of B cell responses and the implication in B cell-related diseases
... maturation protein-1 (Blimp-1) is a critical tran- scription regulator of plasma cell formation, which mainly functions as a transcription repressor ...finger protein 1 (Prdm1) gene, in mice impaired plasma ...
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