Brucella ovis
Comparison of lipopolysaccharide and outer membrane protein lipopolysaccharide extracts in an enzyme linked immunosorbent assay for the diagnosis of Brucella ovis infection
... Comparison of Lipopolysaccharide and Outer Membrane ProteinLipopolysaccharide Extracts in an Enzyme-Linked Immunosorbent Assay for the Diagnosis of Brucella ovis Infection Depar-taonient[r] ...
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Studies on the vaccination of sheep against Brucella ovis infection : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Massey University
... The observat ion o f Hart ley e t . al . ( 1955 ) tha t faecal material i s some t imes p resent in the prepuce o f rams , and the fac t that ram to ram t ransmi ssion of the disease occurs in the absence o f ewes ( ...
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Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model
... B. ovis ⌬abcBA strain in mice (22), this candidate vaccine strain was encapsulated in alginate microcapsules, to overcome the lack of per- ...withB. ovis ⌬ abcBA encapsulated in sterile alginate, thus ...
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Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil
... B. ovis, serologic analyses detected antibodies anti-B. ovis by AGID and indirect ...B. ovis shedding in urine of rams was confirmed by ...B. ovis detection by indirect ELISA using BP26r, ...
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The CO2-dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrases
... of Brucella ovis and most Brucella abortus biovars 1–4 ...reflects Brucella metabolism, an area of active ...B. ovis, and that a modified CAII sequence explains the CO 2 ‑independent ...
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Studies on Brucella ovis infection in deer : a thesis presented in fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Clinical Science at Massey University, Palmerston North, New Zealand
... 5. Brucella avis was isolated from the semen of stag R4 collected on Day 56, but not from the reproductive organs of this stag collected at slaughter on Day ...
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Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice
... these Brucella spp. [5]. In B. ovis , the LPS of the mutants ΔwadB and ΔwadC lacks a part of the core oligosaccharide (component I in Figure 2) and the defects generate a marked increase in the negative ...
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Characterization of Brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme linked immunosorbent assay
... ovis lipopolysaccharide gave better identification of infections and fewer falsenegative results than the enzyme-linked immunosorbent assay with sonicated antigen or the complement fixat[r] ...
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Profiles of serological reactivity against cytosoluble antigens of Brucella ovis in experimentally infected rams
... In contrast to the reactivity patterns shown by native high-pressure liquid chromatography-fractionated cytosolic supernatant antigens, immunoblotting of C and D polypeptides generated b[r] ...
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Rev1 wbdR tagged vaccines against Brucella ovis
... The results presented in this work demonstrate that the O-PS of vaccine Rev1 can be antigenically tagged by genomic insertion of wbdR, the acetyltransferase gene involved in the synthesis of the O-PS repeating unit of E. ...
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Evaluation in mice of Brucella ovis attenuated mutants for use as live vaccines against B. ovis infection
... B. ovis vaccines (Figures 1B and 2) would initially predict a better protective activity for the former ...B. ovis Δomp25d and Δomp22 against an experimental B. ovis infection was equivalent to that ...
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Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp.
... by Brucella spp. and in experimentally infected rams (Brucella ovis infected), rabbits (Brucella melitensis infected), and mice ...and Brucella abortus ...from Brucella natural ...
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Multiple Locus Variable Number Tandem Repeat Analysis Genotyping of Human Brucella Isolates from Turkey
... human Brucella isolates from Turkey, indicated that Turkish human Brucella melitensis isolates were most closely related to the neighboring coun- tries’ isolates included in the East Mediterranean ...
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Postreplication Roles of the Brucella VirB Type IV Secretion System Uncovered via Conditional Expression of the VirB11 ATPase
... ABSTRACT Brucella abortus, the bacterial agent of the worldwide zoonosis brucellosis, primarily infects host phagocytes, where it undergoes an intracellular cycle within a dedicated membrane-bound vacuole, the ...
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Genetic Bases of the Rifampin Resistance Phenotype in Brucella spp
... 21. Paulsen, I. T., R. Seshadri, K. E. Nelson, J. A. Eisen, J. F. Heidelberg, T. D. Read, R. J. Dodson, L. A. Umayam, L. M. Brinkac, M. J. Beanan, S. C. Daugherty, R. T. Deboy, A. S. Durkin, J. F. Kolonay, R. Madupu, W. ...
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Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy
... Our data suggest the use of one or both molecular ap- proaches, rpoB sequencing and MLVA-16 assay, according to the aims of the investigation, because different information is obtained from each approach. rpoB sequencing ...
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MIXED TWO VARIANT TYPES OF CRYOGLOBULINEMIA ASSOCIATED WITH BRUCELLOSIS HUMAN PATIENTS
... for brucella abortus , theoretically may be in spectrum of wide range of immunogenicity possibly include ,th1,th2,b,allergene and aut reactive as well as tolerogenic ...
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Assay dependence of Brucella antibody prevalence in a declining Alaskan harbor seal (Phoca vitulina) population
... to Brucella bacteria, pathogens of concern in marine mammals in ...of Brucella bacteria, which are known to be present in harbor seals in Southeast Alaska ...[12]. Brucella bacteria have been ...
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Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics
... and Brucella melitensis 16 M (GenBank accession numbers AL844501 to AL844509, AE014185 to AE014188, AE001362, FR796433 to FR796468, NC_003143, AL123456, NC_003317, and ...
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A 6 Nucleotide Regulatory Motif within the AbcR Small RNAs of Brucella abortus Mediates Host Pathogen Interactions
... In Brucella abortus, two small RNAs (sRNAs), AbcR1 and AbcR2, are re- sponsible for regulating transcripts encoding ABC-type transport ...for Brucella virulence, as a double chromosomal deletion of both ...
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