budded virus
The Autographa californica Multiple Nucleopolyhedrovirus ORF78 Is Essential for Budded Virus Production and General Occlusion Body Formation
... Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the type species of the genus Alphabaculovirus. The infection cycle of AcMNPV includes two distinct viral pheno- types, budded virus (BV) and ...
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Autographa californica Multiple Nucleopolyhedrovirus me53 (ac140) Is a Nonessential Gene Required for Efficient Budded-Virus Production
... repair virus (AcRepME53:HA-GFP) carrying me53 with a C-terminal hemagglutinin (HA) tag, under the control of its native early and late promoter ...in budded-virus (BV) production compared to both the ...
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The Autographa californica M Nucleopolyhedrovirus ac79 Gene Encodes an Early Gene Product with Structural Similarities to UvrC and Intron-Encoded Endonucleases That Is Required for Efficient Budded Virus Production
... that budded virus (BV) production was reduced in the ac79-knockout virus compared to control viruses, following either virus infection or the transfection of bacmid ...ac79-knockout ...
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Autographa californica Multiple Nucleopolyhedrovirus orf132 Encodes a Nucleocapsid-Associated Protein Required for Budded-Virus and Multiply Enveloped Occlusion-Derived Virus Production
... occlusion-derived virus and budded ...in virus replication, ac132 was deleted from the viral genome by using a bacmid ...infectious budded virus was reduced to an undetectable ...
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The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function
... B aculoviruses are pathogens that infect insects from the orders Lepidoptera, Diptera, and Hymenoptera (1). To date, 60 bacu- lovirus genomes have been fully sequenced, and all of them con- tain the 37 core genes (2) ...
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Early Synthesis of Budded Virus Envelope Fusion Protein GP64 Enhances Autographa californica Multicapsid Nucleopolyhedrovirus Virulence in Orally Infected Heliothis virescens
... occlusion-derived virus (ODV) that establishes primary infection in the midgut following its ingestion by host larvae contains multiple nucleocapsids, all of which enter the same midgut ...the budded ...
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Trichoplusia ni Kinesin-1 Associates with Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Proteins and Is Required for Production of Budded Virus
... The mechanism by which nucleocapsids of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) egress from the nucleus to the plasma membrane, leading to the formation of budded virus (BV), is not ...
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Autographa californica Multiple Nucleopolyhedrovirus Ac92 (ORF92, P33) Is Required for Budded Virus Production and Multiply Enveloped Occlusion-Derived Virus Formation
... of virus-infected cells and to determine if deletion of ac92 had any effect on occlusion morphogenesis, polyhedrin and enhanced green fluorescent protein (egfp) were inserted into the polyhedrin locus by site- ...
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The F-Like Protein Ac23 Enhances the Infectivity of the Budded Virus of gp64-Null Autographa californica Multinucleocapsid Nucleopolyhedrovirus Pseudotyped with Baculovirus Envelope Fusion Protein F
... To see to what extent Ac23 contributes to the production of infectious BVs, one-step growth curves of vAc-egfp, vAc gp64⫺ - SeF, vAc gp64⫺ Ac23⫺ -SeF, and vAc gp64⫺ Ac23⫺ -gp64-Ac23 were made and compared (Fig. 2B). Sf9 ...
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Autographa californica Multiple Nucleopolyhedrovirus AC83 is a Per Os Infectivity Factor (PIF) Protein Required for Occlusion-Derived Virus (ODV) and Budded Virus Nucleocapsid Assembly as well as Assembly of the PIF Complex in ODV Envelopes
... To assess the impact of the AC83 deletions on BV production, virus titers were determined at 24 and 48 h postinfection (hpi) for each construct (Fig. 4). At 24 hpi, all of the viruses produced BV levels lower than ...
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Autographa californica Multiple Nucleopolyhedrovirus ORF11 Is Essential for Budded-Virus Production and Occlusion-Derived-Virus Envelopment
... knockout virus Ac11KO. To construct the ac11 knockout virus (Ac11KO), the AcMNPV recombinant bacmid Ac-MK (13), in which the ...recombinant virus Ac11KO, in which the ac11 gene was disrupted with a ...
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Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
... in budded virus but not in virus-producing cells when either primary rhesus macrophages or CEM ⫻ 174 cells were used in similar assays (data not shown), indicating that the SIV Gag protein phos- ...
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Immediate-Early Protein ME53 Forms Foci and Colocalizes with GP64 and the Major Capsid Protein VP39 at the Cell Membranes of Autographa californica Multiple Nucleopolyhedrovirus-Infected Cells
... in budded-virus production ...in budded-virus production have not yet been ...repair virus to demonstrate the localization pat- tern of ME53:GFP throughout the infection cycle in two ...
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Proteomics of the Autographa californica Nucleopolyhedrovirus Budded Virions
... the budded virus (BV) and the oc- clusion-derived virus ...the virus among cells and tissues in the host ...the virus buds out (BV). BVs acquire an envelope of virus-encoded ...
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Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications
... The BEVS uses insect viruses from the family Bacu- loviridae and their hosts, allowing for large-scale recombinant protein expression (Palomares et al., 2015). As discussed in Chapter 9, the Baculoviridae is a family of ...
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Identification of BV/ODV-C42, an Autographa californica Nucleopolyhedrovirus orf101-Encoded Structural Protein Detected in Infected-Cell Complexes with ODV-EC27 and p78/83
... C42 is present in a complex with p78/83 and EC27. orf101 (C42) was first drawn to our attention when a yeast two-hybrid screen of infected-cell cDNA libraries (18 and 24 h p.i.), using orf144 (EC27) as bait, identified ...
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AN EMERGING PARADIGM EXOSOMES: COMPOSITION, BIOLOGICAL FUNCTIONS, AND DIAGNOSTIC AND THERAPEUTIC POTENTIALSAbdul Mannan, Syed Aun Muhammad, Fareeha Anwar, Imran Ameerzada, Nisar ur Rehman, Nighat Fatima, Tariq Ismail*DOWNLOAD/VIEW/http://doi.org
... it performs some unknown function. It has been reported that exosomes are involved in Dictyostelium cells migration by using chemo-attractant signals (37). One another group of researchers studied the level of miRNA in ...
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Curvature recognition and force generation in phagocytosis
... Figure 10 Myosin-II engagement in particle release. Cells interacting with budded yeast express GFP -myosin-II heavy chains (green) and the mRFP -LimE Δ label for filamentous actin (red). Positions of the yeast ...
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Role of the Ebolavirus Glycoprotein in Countering Tetherin During Viral Budding
... In 2008, Tetherin was shown to have anti-viral activity by inhibiting budding of HIV-1 particles (183, 184). Shortly thereafter, our lab demonstrated that EBOV particles are also retained in a Tetherin-dependent manner ...
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Chicken anemia virus an economically important poultry virus
... Chicken anaemia virus is a small unique among animal viruses having the diameter of 25 nm.19 Genome consists of circular single-stranded DNA.20The DNA multiplied in infected cells via Rolling-circle model. CAV ...
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