BV2 cells
RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells
... and BV2 cells to LPS To validate the findings of RNA-Seq, we compared the functions between primary microglia and BV2 ...the cells were quiescent and the method was consistent and repeat- ...
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Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6
... end, BV2 microglial cells were co-cultured with MSCs and rmTSG- 6 at different concentrations for 6 ...LPS-stimulated BV2 cells were cultured in the presence of ...the BV2 microglia ...
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Adiponectin suppresses amyloid-β oligomer (AβO)-induced inflammatory response of microglia via AdipoR1-AMPK-NF-κB signaling pathway
... resting cells, NF-κB is retained in the cytoplasm by interacting directly with IκBα, an inhibitor protein in the IκB ...neuroblastoma cells overex- pressing AβO from cytotoxicity under oxidative stress and ...
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Ethyl Pyruvate Inhibits HMGB1 Phosphorylation and Release by Chelating Calcium
... of BV2 cells were confirmed by LDH (Figure 3B) and NO assays (Figure 3C), ...of BV2 cells was further confirmed by showing that iNOS (see Figure 3C) and proinflammatory marker expressions ...
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Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD
... the manufacturer's instructions. Briefly, cDNA samples (100 ng, based on the original RNA concentrations) were brought to a total volume of 22.5 μ l using RNase-free water and mixed with 25 μl of 2X TaqMan Universal Mas- ...
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Mesenchymal stem cells exert anti-proliferative effect on lipopolysaccharide-stimulated BV2 microglia by reducing tumour necrosis factor-α levels
... glial cells at the site of injury ...LPS-stimulated BV2 microglia in ...of BV2. Also, the basal proliferation of BV2 cells may not require TNF-α, as addition of anti-TNF onto ...
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Neuromelanin activates proinflammatory microglia through a caspase-8-dependent mechanism
... in BV2 microglia in response to classical proinflammogens, including lipopolysaccharide (TLR4 agonist), lipoteichoic acid (TLR2 agonist), PamC3sk4 (synthetic lipopeptide TLR1/2 agonist), and IFNγ, in contrast with ...
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Effects of ginsenoside Re on LPS induced inflammatory mediators in BV2 microglial cells
... crystals. BV2 cells were plated on 96-well plates (2 × 10 4 ...the BV2 cells were treated with the indicated concentrations of G-Re for 24 h prior to 1 μg/ml of LPS treatment for an additional ...
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Endotoxin-activated microglia injure brain derived endothelial cells via NF-κB, JAK-STAT and JNK stress kinase pathways
... bEND.3 cells, cocul- tures of bEND.3 cells with BV2 cells led to LPS induced injury to ...bEND.3 cells (Figure 7A-C) and NO accumula- tion (Figure ...activated BV2 cells ...
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Urate inhibits microglia activation to protect neurons in an LPS-induced model of Parkinson’s disease
... Microglia secrete proinflammatory cytokines such as NO, TNF-α, and IL-1β upon LPS stimulation [43, 44]. Here, we found that urate inhibited the release of inflam- matory factors including NO, TNF-α, and PGE2 while ...
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<p>Dexmedetomidine Attenuates Neuroinflammation In LPS-Stimulated BV2 Microglia Cells Through Upregulation Of miR-340</p>
... in BV2 cells via inhibiting NF-κB. (A) BV2 cells were treated with 20 µg/mL Dex for 48 ...in BV2 cells were detected by RT-qPCR. (B) BV2 cells were transfected with ...
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Oxysterol levels and metabolism in the course of neuroinflammation: insights from in vitro and in vivo models
... glial cells (containing microglia and ...glial cells in response to the inflammatory stimuli [54, ...glial cells after both 8 and 24 h of LPS incu- bation in order to be able to compare the results ...
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Transcription factor myocyte enhancer factor 2D regulates interleukin-10 production in microglia to protect neuronal cells from inflammation-induced death
... 18 h after LPS treatment (Figure 3A, bottom graph). Thus, the pattern of IL-10 mRNA response matched well with that of MEF2D protein and prompted us to search for possible MEF2 regulatory target in IL-10 gene promoter. ...
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Determining the role of IL-4 induced neuroinflammation in microglial activity and amyloid-β using BV2 microglial cells and APP/PS1 transgenic mice
... BV2 cells were grown on glass coverslips in 12-well plates until 80% confluency after approximately 3 days from ...experiment, cells were starved and treated as above. After incubation cells ...
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A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia
... for BV2 microglia cultured in ...[20,24,25], BV2 microglia in 3D cultures significantly increased expression of the cell surface receptor CD40, nitric oxide (NO), IL-6, TNF- α and ...both cells and ...
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Reciprocal interactions of mouse bone marrow-derived mesenchymal stem cells and BV2 microglia after lipopolysaccharide stimulation
... and BV2 cells were then seeded at 5 × 10 3 BV2 ...harvesting. Cells were harvested onto a filter mat by using an automated cell harvester (Harvester Mach III M; TOMTEC, CT, ...
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Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells
... of BV2 cells with LPS led to rapid activation of p38 MAPK, ERK, and JNK, with peak levels of each phospho-MAPK occurring 15 to 60 min after addition of ...LPS-stimulated BV2 microglia (Figure ...
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The protective effect of fermented Curcuma longa L on memory dysfunction in oxidative stress induced C6 gliomal cells, proinflammatory activated BV2 microglial cells, and scopolamine induced amnesia model in mice
... glial cells, including astrocytes and microglial cells, play critical roles in supporting and protecting ...Glial cells are abundant in the hippo- campus and cortex, which control the ability for ...
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Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming
... experiments: BV2 and whole 3D spheroids MSCs and BV2 cells were seeded and treated as de- scribed; however, after the priming treatment with IL-1, MSC spheroids were washed twice with PBS and placed ...
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THE INHIBITORY EFFECT OF GALANGIN ON CYTOKINES AND NITRIC OXIDE IN MICROGLIA BV2 CELL LINE
... microglia BV2 after being induced with lipopolysaccharide. Microglia BV2 was incubated with Galangin (with and without LPS) and dexamethasone for ...of BV2 macroglia at concentrations less than 40 ...
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