C. difficile
Detection of Toxigenic Clostridium difficile: Comparison of the Cell Culture Neutralization, Xpert C difficile, Xpert C difficile/Epi, and Illumigene C difficile Assays
... of C. difficile are mucosal damage to the colon that is caused by toxin A and/or toxin ...toxigenic C. difficile organisms, tests for which the time to final result can be 48 to 72 ...for ...
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Recombinant lipoprotein-based vaccine candidates against C. difficile infections
... different C. difficile vaccine strategies are being evaluated; the most advanced being vaccination with formalin-inactivated toxins ...of C. difficile toxins as an antigen formulated with ...
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Multicenter Clinical Evaluation of the Portrait Toxigenic C difficile Assay for Detection of Toxigenic Clostridium difficile Strains in Clinical Stool Specimens
... the C. difficile “common antigen” glu- tamate dehydrogenase (GDH) have been used to screen for the presence of ...C. difficile in stool specimens; however, they lack sen- sitivity (90% to 93%) ...
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Evaluation of a New Automated Homogeneous PCR Assay, GenomEra C difficile, for Rapid Detection of Toxigenic Clostridium difficile in Fecal Specimens
... toxigenic C. difficile, being more sensitive than IA tests and EIAs and significantly faster than the standard meth- ods (24, 36, ...toxigenic C. difficile among children (40, 41) and elderly ...
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Epidemiology of Clostridium difficile infection in hospitalized adults and the first isolation of C difficile PCR ribotype 027 in central China
... Practice guidelines indicated the two biggest risk factors of CDI were exposure to antibiotics and the organism [40]; Gualtero et al.’s research [41] demonstrated chronic kidney disease was the most common comorbidities ...
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Circulating antibody and memory B cell responses to C difficile toxins A and B in patients with C difficile associated diarrhoea, inflammatory bowel disease and cystic fibrosis
... toxigenic C. difficile. The development of C. difficile infection in two cystic fibrosis patients suggest that immune-mediated protection can be overcome and may reflect tilting of the balance ...
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Do piperacillin/tazobactam and other antibiotics with inhibitory activity against Clostridium difficile reduce the risk for acquisition of C difficile colonization?
... of C. difficile strain VA 17 and the mean (+/− standard error) change in ...C. difficile concentration, stratified by the type of antibiotic ...of C. difficile growth (71 versus ...
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Multicenter Evaluation of the Quidel Lyra Direct C difficile Nucleic Acid Amplification Assay
... In addition to the fact that there are some performance differ- ences between thermocycling platforms, the Lyra assay also has a few other minor drawbacks. These include the fact that it does not identify specific ...
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In vitro susceptibility of Clostridium difficile to SMT19969 and comparators, as well as the killing kinetics and post-antibiotic effects of SMT19969 and comparators against C. difficile
... Cultures of C. difficile were prepared in 15 mL polypropylene centrifuge tubes in the presence of DMSO or antibiotic, as described above. Cultures were incubated for 1 h before being collected by ...
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Asymptomatic and yet C. difficile-toxin positive? Prevalence and risk factors of carriers of toxigenic Clostridium difficile among geriatric in-patients
... Intestinal microbiome is increasingly being recognized as an important health factor. Its potential role for colonization and infection with Clostridum difficile seems to be of high importance. The intestinal ...
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Comparison of GenomEra C difficile and Xpert C difficile as Confirmatory Tests in a Multistep Algorithm for Diagnosis of Clostridium difficile Infection
... Therefore, 979 specimens from 799 patients were analyzed (Fig. 1). Toxigenic C. difficile was detected in 117 (11.9%) speci- mens from 96 patients using the gold standard procedure. Molec- ular systems were ...
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Microbiome Data Distinguish Patients with Clostridium difficile Infection and Non C difficile Associated Diarrhea from Healthy Controls
... We found distinct differences in the microbiomes of people with and without CDI as well as with and without diarrhea. We devel- oped classification models to differentiate whether individuals had C. ...
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Rapid Molecular Characterization of Clostridium difficile and Assessment of Populations of C difficile in Stool Specimens
... of C. difficile directly from stool specimens is the testing route of choice at the Wadsworth Center for the rapid identification of putative genetic factors of ...that C. difficile could not ...
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Efficacy of cleaning products for C difficile
... existing C difficile infection rates at the facilities enrolled in the ...for C dif- ...for C difficile (P = ...for C dif- ficile before decontamination and 7 (3%) were positive ...
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Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C difficile associated disease
... Clinical results showed that the cytotoxin assay is the most sensitive test 94.1% for the diagnosis of CDAD, followed by EIA 85.3%, toxigenic culture 79.4%, and latex agglutination 58.8%[r] ...
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Evaluation of Copan FecalSwab as Specimen Type for Use in Xpert C difficile Assay
... ABSTRACT Liquid-based microbiology (LBM) devices incorporating flocked swabs and preservation medium ease transport of specimens and improve specimen yield compared to traditional fiber wound swabs; however, the ...
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Risk factors for Clostridium difficile infection in surgical patients hospitalized in a tertiary hospital in Belgrade, Serbia: a case–control study
... Among the main findings of this study are the identifi- cation of significant independent RFs for CDI in surgical patients, including carbapenems, admission to ICU and the 3rd generation of cephalosporins. The disruption ...
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Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level
... The proteins involved in the assembly of C. difficile fla- gellum and Pro–Pro endopeptidase were present among other proteins revealing higher expression. The quantifi- cation of the proteins involved in ...
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Isolation of Toxin Producing Clostridium difficile from Two Children with Oxacillin- and Dicloxacillin-Associated Diarrhea
... The diarrhea ceased within two days and repeated stool cultures at that time were negative for C difficile and no. cellular cytotoxicity was observed[r] ...
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Genotyping of outbreak related and sporadic isolates of Clostridium difficile belonging to serogroup C
... well by PFGE and almost as well by the RAPD assay, whereas the combination of the two typing methods increased the level of resolution (Table 1). Other authors found the RAPD assay to be nearly as discriminative as PFGE, ...
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