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C. difficile toxin A

Identification of Toxin A Negative, Toxin B Positive  Clostridium difficile by PCR

Identification of Toxin A Negative, Toxin B Positive Clostridium difficile by PCR

... the toxin A gene; primers NK3 and NK2 were derived from the nonrepeating portion of the ...C. difficile toxin A gene, and primers NK11 and NK9 were derived from the repeating portion of the ...

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Risk factors for poor outcome in community-onset Clostridium difficile infection

Risk factors for poor outcome in community-onset Clostridium difficile infection

... positive C. difficile toxin test and diarrhea or an equivocal ...C. difficile toxin test, diarrhea, and treatment for ...identify C. difficile toxin A or B ...

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Neutrophil recruitment in Clostridium difficile toxin A enteritis in the rabbit

Neutrophil recruitment in Clostridium difficile toxin A enteritis in the rabbit

... Clostridium difficile-associated enteritis and ...in C. difficile toxin A-induced ...Pertussis toxin and the nonhydrolyzable GTP analog GTPgamma S both inhibited 3H-toxin A ...

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Correlation of Disease Severity with Fecal Toxin Levels in Patients with Clostridium difficile Associated Diarrhea and Distribution of PCR Ribotypes and Toxin Yields In Vitro of Corresponding Isolates

Correlation of Disease Severity with Fecal Toxin Levels in Patients with Clostridium difficile Associated Diarrhea and Distribution of PCR Ribotypes and Toxin Yields In Vitro of Corresponding Isolates

... a C. difficile clone recently emerging in Canada appears to be asso- ciated with high transmission, attack, and mortality rates ...infecting C. difficile strain may be due to the highly ...

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Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection

Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection

... of C. difficile were ...(the toxin repressor gene) were utilized, while the TechLab Tox A/B ELISA and the tcdB-based PCR assay were used in the initial ...for C. difficile analysis, ...

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Association of Fidaxomicin with C. difficile spores: Effects of Persistence on Subsequent Spore Recovery, Outgrowth and Toxin Production.

Association of Fidaxomicin with C. difficile spores: Effects of Persistence on Subsequent Spore Recovery, Outgrowth and Toxin Production.

... of C. difficile (as has recently been shown for ramoplanin[16]), potentially due to electrostatic charges resulting form cross-linkages on the spore ...of C. difficile spores, and affect ...

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Epidemiology of Clostridium difficile infection in hospitalized adults and the first isolation of C  difficile PCR ribotype 027 in central China

Epidemiology of Clostridium difficile infection in hospitalized adults and the first isolation of C difficile PCR ribotype 027 in central China

... were C. difficile culture positive, and 73 ...toxigenic C. difficile (TCD), with tcdA + tcdB+ strains accounting for ...binary toxin positive and one of them was finally identified as ...

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Diagnosis of Clostridium difficile infection by toxigenic culture and  PCR assay

Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay

... There are various tests for diagnosis of CDI in laboratories. Some of these tests are enzyme immu- noassay (EIA), glutamate dehydrogenase (GDH), cytotoxicity assay (CA), toxigenic culture and PCR. Enzyme immunoassay is a ...

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Asymptomatic and yet C. difficile-toxin positive? Prevalence and risk factors of carriers of toxigenic Clostridium difficile among geriatric in-patients

Asymptomatic and yet C. difficile-toxin positive? Prevalence and risk factors of carriers of toxigenic Clostridium difficile among geriatric in-patients

... Clostridium difficile infections (CDI) are the most frequent cause of diarrhoea in ...Clostridium difficile strains occurs in elderly patients without diarrhoea and whether there is a “ risk pattern ” of ...

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A Multicenter Study of the Revogene C  difficile System for Detection of the Toxin B Gene from Unformed Stool Specimens

A Multicenter Study of the Revogene C difficile System for Detection of the Toxin B Gene from Unformed Stool Specimens

... Collection of C. difficile specimens. Residual, raw, unformed stool samples submitted to the clinical laboratory for C. difficile testing were enrolled at 7 geographically distributed clinical centers within ...

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Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease

Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease

... than toxin assays and may require purchase of expensive equipment ...toxigenic C. difficile will reduce nosocomial transmission and disease and thus generate long-term ...for C. ...

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Detection of Toxigenic Clostridium difficile: Comparison of the Cell Culture Neutralization, Xpert C  difficile, Xpert C  difficile/Epi, and Illumigene C  difficile Assays

Detection of Toxigenic Clostridium difficile: Comparison of the Cell Culture Neutralization, Xpert C difficile, Xpert C difficile/Epi, and Illumigene C difficile Assays

... of C. difficile are mucosal damage to the colon that is caused by toxin A and/or toxin ...toxigenic C. difficile organisms, tests for which the time to final result can be 48 to ...

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Characterization of a Toxin A Negative, Toxin B Positive Strain of Clostridium difficile Responsible for a Nosocomial Outbreak of Clostridium difficile Associated Diarrhea

Characterization of a Toxin A Negative, Toxin B Positive Strain of Clostridium difficile Responsible for a Nosocomial Outbreak of Clostridium difficile Associated Diarrhea

... Clostridium difficile-associated diarrhea (CAD) is a very common nosocomial infection that contributes significantly to patient morbidity and mortality as well as to the cost of ...of toxin A-negative, ...

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Characteristics, Treatment, and Outcomes Associated with Clostridium difficile Associated Diarrhea in a Veterans Affairs Medical Center

Characteristics, Treatment, and Outcomes Associated with Clostridium difficile Associated Diarrhea in a Veterans Affairs Medical Center

... binary toxin has been present in the epidemic strain though its exact role is not well defined [8] [9] [12] ...binary toxin gene and more severe cases of C. difficile associated diarrhea ...of ...

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Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary Phase Autolysis Resulting in Toxin Release in Clostridium difficile

Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary Phase Autolysis Resulting in Toxin Release in Clostridium difficile

... analyzed by matrix-assisted laser desorption ionization–time of flight mass spectrom- etry (MALDI-TOF MS) and by MALDI-TOF/TOF tandem MS (MS/MS), and the resulting data were used to predict consistent structures (Table ...

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RstA Is a Major Regulator of Clostridioides difficile Toxin Production and Motility

RstA Is a Major Regulator of Clostridioides difficile Toxin Production and Motility

... RstA DNA-binding activity requires the species-specific C-terminal domains. The observation that RstA does not bind to target DNA in the tested in vitro conditions but does bind DNA in cell lysates suggests that a ...

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Bacteriophage-Mediated Toxin Gene Regulation in Clostridium difficile

Bacteriophage-Mediated Toxin Gene Regulation in Clostridium difficile

... gested that a significant amount of transcriptional read- through occurs, resulting in bicistronic transcripts, including tcdB-E (18). RepR may be regulating tcdE through TcdR reg- ulation of tcdB. Thus, reduced ...

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Molecular and Susceptibility Analysis of Toxigenic Clostridium difficile Obtained from Adult Patients Suspected of CDI in Trinidad

Molecular and Susceptibility Analysis of Toxigenic Clostridium difficile Obtained from Adult Patients Suspected of CDI in Trinidad

... the C. difficile housekeep- ing gene (see Figure ...the toxin A gene, which differed significantly from the expected 1266 bp fragment as seen for the ATCC 9689 ...C. difficile control ...

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Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level

Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level

... of C. difficile isolates and supernatant precipitation Clostridium difficile isolates were recovered from the frozen stocks by inoculating on the Schaedler Anaer- obe Agar CM0437 (Oxoid) and cultured ...

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Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification

Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification

... The LAMP detecting tcdB in DNA extracted directly from stool specimens proved to be a reliable assay when the test results were compared with those for detection of fecal TcdB and C. difficile culture. ...

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