C II, type II collagen
Measurement of serum and vitreous concentrations of anti-type II collagen antibody in diabetic retinopathy
... as C-reactive protein; 2) increased vitreous levels of inflammatory cytokines such as tumor necrosis factor alpha, interleukin (IL)-1, and IL-6; and 3) infiltration of inflammatory cells such as neutrophils, ...
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The trans-well coculture of human synovial mesenchymal stem cells with chondrocytes leads to self-organization, chondrogenic differentiation, and secretion of TGFβ
... SMSC were kept in monolayer cultures alone or in co- culture with CHDR. As a positive control, these cocul- tures were supplemented with TGFβ. While SMSC alone stayed separated after 1 week, an aggregation of SMSC was ...
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Soluble PD 1 aggravates progression of collagen induced arthritis through Th1 and Th17 pathways
... chick type II collagen (CII; Sigma-Aldrich) overnight at 4 °C in 10 mM acetic acid and combining it with an equal vol- ume of complete Freund’s adjuvant containing 5 mg/ml heat-killed ...
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Evidence for altered synthesis of type II collagen in patients with osteoarthritis
... of type II col- lagen is increased in osteoarthritis ...the C-propeptide of type II procollagen (CPII), released extracellularly from the newly synthesized molecule, is directly related ...
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The response to oestrogen deprivation of the cartilage collagen degradation marker, CTX II, is unique compared with other markers of collagen turnover
... rat type II collagen ...the C-telopeptide of type II ...GST-human type II procollagen exon 2 fusion protein [28], and which cross-react with rat type ...
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Kniest dysplasia is characterized by an apparent abnormal processing of the C propeptide of type II cartilage collagen resulting in imperfect fibril assembly
... the C-propeptide of type II collagen (chondrocalcin) from the extracellular matrix of epiphyseal cartilages, although it was detected (as in normal cartilages) in the lower hypertrophic zone ...
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Combined detection of serum CTX-II and COMP concentrations in osteoarthritis model rabbits: an effective technique for early diagnosis and estimation of disease severity
... crosslinked C-telopeptides of type II collagen (CTX-II) are two markers which can potentially predict the destruction of articular cartilage of early ...
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Collagen types I and II distribution: a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised rabbit knee joints
... of type I and type II ...monoclonal collagen type I antibody (Clone ...monoclonal collagen type II antibody (Clone No. II-II6B3-s and cII cI-c, ...
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Type 1 regulatory T cells specific for collagen type II as an efficient cell based therapy in arthritis
... Col II–spe- cific TCR transgenic mice in the presence of IL-10 as pre- viously described for antigen-specific Tr1 clones in both mice and humans ...Col II–specific TCR Vβ8 and CD4 expression (Figure 1A) as ...
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Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model
... We coated the microplate with protein from the rabbit’s ure- thra in buffer coating for 2 hours at room temperature. The sample was washed with washing buffer (PBST) 3 times for 3 minutes each. After that, 50 µL BSA 1% ...
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Safety, tolerability and efficacy of intra articular Progenza in knee osteoarthritis: a randomized double blind placebo controlled single ascending dose study
... [urine: type II collagen C2C pep- tide (C2C) and C-terminal telopeptide of type II collagen (CTX-II); serum: macrophage migration inhibitory factor (MIF), HA and ...
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Exposure to Mimivirus Collagen Promotes Arthritis
... mimivirus collagen and the development of autoim- munity was corroborated by the occurrence of IgG against mimi- virus collagen among rheumatoid arthritis ...virus collagen and to a breakdown of im- ...
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Patella Alta and Trochlea Dysplasia Is Associated with Abnormal Type II Collagen and Matrix Accumulation in Chondrocytes
... abnormal type II collagen fibrils in ag- gregates and chondrocytes with abnormal matrix accumulation in rough Endoplasmic Reticulum ...that type II collagen was deposited ...
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Spinacia oleracea extract attenuates disease progression and sub chondral bone changes in monosodium iodoacetate induced osteoarthritis in rats
... Pathophysiology of OA, is also associated with increased inflammatory cytokines associated with the disruption of homeostasis of ECM component in OA as it is often sub- jected to target high mechanical load tissues such ...
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Molecular and ultrastructural studies of a fibrillar collagen from octocoral (Cnidaria)
... Briefly, hydrolysates were prepared by treating the crushed fibers with several volumes of tri-sodium citrate di-hydrate for a short time and then diluting this mixture using deionized water. Internal standards were then ...
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Humoral and Cellular Sensitivity to Collagen in Type II Collagen Induced Arthritis in Rats
... developed type-specific cellular as well as humoral ...was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or ...
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Decreased hypertrophic differentiation accompanies enhanced matrix formation in co cultures of outer meniscus cells with bone marrow mesenchymal stromal cells
... against collagen types I and ...against collagen I (MAB3391) from Millipore (Temecula, CA, USA) or collagen II (II-II6B3) from the Developmental Studies Hybridoma Bank at the University ...
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Repair of a chondral defect using a cell free scaffold in a young patient - a case report of successful scaffold transformation and colonisation
... on the observations of Lee et al. [26], who showed that chondrocytes can interact with surrounding collagen fibres by bending them, and thus they could possibly move along these fibres. The other is based on the ...
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Early and stable upregulation of collagen type II, collagen type I and YKL40 expression levels in cartilage during early experimental osteoarthritis occurs independent of joint location and histological grading
... by referring data to the DNA content of the tissue, we referred specific mRNA expression levels to expression of the housekeeping gene GAPDH. The strength of our method is that we can detect a specific regulation of ...
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Pseudoachondroplasia is caused through both intra and extracellular pathogenic pathways
... line from Amersham International (Buckinghamshire, United Kingdom); culture media and FBS from Gibco (Eggenstein, Germany); the mouse monoclonal (9E10) anti–c-myc Ab from Santa Cruz Biotechnology (Hei- delberg, ...
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