c-Jun N-terminal kinase 2
Knockout of the c-Jun N-terminal Kinase 2 aggravates the development of mild chronic dextran sulfate sodium colitis independently of expression of intestinal cytokines TNFα, TGFB1, and IL-6
... for 2 hours at room temperature: monoclonal rat anti-CD4 antibody (BD Biosci- ences, Heidelberg, Germany) diluted 1:400 ...for 2 hours at room temperature and then at 4 ° C ...
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Differential roles of p38 MAPK and JNKs in mediating early protection or apoptosis in the hyperthermic perfused amphibian heart
... protein kinase (p38-MAPK) and c-Jun N-terminal kinases (JNKs) by hyperthermia was investigated in the isolated perfused Rana ridibunda ...° C) was found to profoundly stimulate ...
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Varicella-Zoster Virus Infection of Human Fibroblast Cells Activates the c-Jun N-Terminal Kinase Pathway
... acidic vacuoles (21), so virus was grown in a MeWo mutant cell line, MPR-KD, in which the mannose-6-phosphate receptor, involved in trafficking to late endosomes and lysosomes, is knocked down (7). Virions were isolated ...
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Ubiquitin-specific protease 14 regulates c-Jun N-terminal kinase signaling at the neuromuscular junction
... Figure 7 Inhibition of pJNK improves the structural and functional deficits caused by inhibition of USP14 ’ s DUB activity. (A) Representative immunoblots of pJNK1/2 and JNK1/2 in spinal cords from ...
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Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I
... homolog 2 (EZH2) mRNA was measured by quantitative real time PCR ...protein kinase/c-Jun N-terminal kinase (SAPK/JNK), p65, DNA methyltransferase 1 (DNMT1) and ...
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Original Article Puerarin modulates autophagy to ameliorate cerebral ischemia/reperfusion injury through JNK signaling pathway
... Abstract: Puerarin, a major isoflavone in radix puerariae, exerts remarkable pharmacological effects on ischemic stroke patients in the clinic. However, the underlying mechanisms of the protective effects of puerarin are ...
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c-Jun N-terminal kinase activation has a prognostic implication and is negatively associated with FOXO1 activation in gastric cancer
... Nine paraffin array blocks were prepared by Superbio- chips Laboratories (Seoul, Korea), as previously de- scribed [32]. Briefly, core tissue biopsies (2 mm in diameter) were taken from individual ...
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C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced by oxidative stress
... We examined whether inhibition of JNK also affected localization of TDP-43 in cells exposed to sodium arsenite, the most commonly used SG inducer. Sodium arsenite is also known to induce JNK activation [41]. Initially, ...
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Regulation of inflammatory arthritis by the upstream kinase mitogen activated protein kinase kinase 7 in the c Jun N Terminal kinase pathway
... 0.05, n = 3 for each group) (Figure ...The c-Jun protein levels were higher in the control ASO-treated mice compared with MKK7 treat- ment due to increased local cytokine production, such as IL-1 b ...
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Activation of c-jun N-terminal kinase in spinal cord contributes to breast cancer induced bone pain in rats
... The JNK inhibitor SP600125 (1,9-Pyrazoloanthrone) was purchased from Calbiochem (San Diego, California, USA). SP600125 stock solution was prepared in DMSO at a concentration 20 μg/μl and stored at -20°C until use. ...
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c Jun N terminal kinase activity supports multiple phases of 3D mammary epithelial acinus formation
... generating the spheres in the presence of a MEK-ERK signalling inhibitor. Interestingly, during normal acinus formation, ERK-specific phosphatase (MKP-2 and -3) expression was induced and this may also support the ...
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Flavonoids in modulation of cell survival signalling pathways
... ERK, c-Jun N-terminal kinase (JNK), and p38, MAPKs (Johnson and Lapadat ...protein kinase (SAPK), is an important member of the MAPKs super- family, the members of which are ...
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c Jun N terminal kinase activation by nitrobenzoxadiazoles leads to late stage autophagy inhibition
... factor 2 (TRAF2) and the c-Jun N-terminal kinase (JNK) [2, 3, 13, ...signal-regulating kinase (ASK1), which in turn activates both p38 and JNK mito- gen-activated ...
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Human Endotoxemia Activates p38 MAP Kinase and p42/44 MAP Kinase, But Not c-Jun N-terminal Kinase
... Protein Kinase (MAPK) family are critically involved in intracellular signal trans- duction, mediating cell responses to a variety of dif- ferent infectious and inflammatory ...MAPK, c-jun ...
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Original Article The C-jun N-terminal kinase signaling pathway regulates cyclin D1 and cell cycle progression in airway smooth muscle cell proliferation
... eration, while NF-κB and p38 do not. Cell proliferation was assessed by crys- tal violet assay (A) and tritiated-thymidine incorporation (B). Normal-n1, nor- mal human airway smooth muscle cells 1; Normal-n2, normal ...
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c Jun N terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis
... Western blot analysis. Synoviocytes were pretreated with medium or MAPK inhibitors for 30 minutes and fur- ther incubated with IL-1 (2 ng/ml) for 15 minutes. Pro- tein samples (25 µg/lane) from FLS were run on a ...
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Inhibition of c Jun N terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
... The downstream molecules of JNK are pro- apoptotic factors from the Bcl-2 family of proteins (Bax, Bak, Bad) (Vlahopoulos and Zoumpourlis, 2004; Chu et al., 2009; Du Pasquier et al., 2011). The activated ...
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Anti-high mobility group box-1 (HMGB1) antibody attenuates kidney damage following experimental crush injury and the possible role of the tumor necrosis factor-α and c-Jun N-terminal kinase pathway
... 3, 2, and 2 in the 0 h, 12 days, 1 day, and 2 days groups, ...5, 2, 3, and 2 deaths were observed in the CS, CS + vehicle, CS + anti- TNF-α antibody, CS + SP600125, and CS + anti-HMGB1 ...
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Inhibition of c-Jun N-Terminal Kinase Attenuates Low Shear Stress-Induced Atherogenesis in Apolipoprotein E-Deficient Mice
... Cytoplasm and nuclear proteins were extracted from three and six contralat- eral carotid artery specimens after undis- turbed and low shear stress treatment, respectively, for each group. HUVECs for each group were ...
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5444.pdf
... Animals were deeply anesthetized with an intraperitoneal injection of urethane (2 mg/kg). Once deeply anesthetized, as monitored by the toe-pinch and eye-blink reflexes, the animals were transcardially perfused ...
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