C-terminal binding protein 1
Cloning and expression of an equine herpesvirus 1 origin-binding protein.
... In order to confirm that the C-terminal DNA-binding domain of EHV-1 OBP was mediating the same sequencespecific binding as the wild-type protein, in vitro-expressed del 1-499 was used to[r] ...
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The N- and C-Terminal Domains of the NS1 Protein of Influenza B Virus Can Independently Inhibit IRF-3 and Beta Interferon Promoter Activation
... a protein consisting of GST fused to the NS1 protein of influenza B/Yamagata/1/73 virus (281 amino ...B/NS1 protein were generated: GST-B/NS1 33/38, 47/50, 52/53/54, 58/60/64, 70/71, 77/78, ...
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Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase
... maltose-binding protein (MBP) and thereafter with HIVIN, with the N-terminal 20 aa residues containing a hexahistidine ...HiTrap protein A column (Amersham Pharmacia Biotech ...
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Cellular Membrane-Binding Ability of the C-Terminal Cytoplasmic Domain of Human Immunodeficiency Virus Type 1 Envelope Transmembrane Protein gp41
... membrane binding ability and the mul- timerization potential of subdomains of the cytoplasmic tail (Table 1) also has implications for understanding the role of the cytoplasmic tail in virus entry into host ...
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APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8
... 3, C and E) but not in proximal tubules (Figure ...increased protein solubility, was immobilized on a biosensor chip. VAMP8 (aa 1–76), which encompasses the SNARE domain (residues 12–72) and the ...
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Novel Structure and Unexpected RNA-Binding Ability of the C-Terminal Domain of Herpes Simplex Virus 1 Tegument Protein UL21
... this protein in vitro has been ob- served previously (21, 30, 36), typically with affinity-tagged pro- tein constructs expressed in ...by binding part- ners of UL21, ...
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<p>C-terminal of E1A binding protein 1 enhances the migration of gastric epithelial cells and has a clinicopathologic significance in human gastric carcinoma</p>
... The C-terminal of E1A binding protein (CtBP) was originally identi fi ed based on its ability to bind the carboxyl terminus of the adenovirus E1A ...two protein isoforms, CTBP1 and ...
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Dimerization of the Papillomavirus E2 Protein Is Required for Efficient Mitotic Chromosome Association and Brd4 Binding
... E2 protein. The resulting fusion protein was tested for its ability to bind Brd4 in the absence or presence of the AP20187 ...NH protein to interact with ...Brd4 binding of the fusion ...
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Redox Potential Regulates Binding of Universal Minicircle Sequence Binding Protein at the Kinetoplast DNA Replication Origin
... DNA binding of N-terminal deleted UMSBP is insensitive to the redox ...the C-terminal DNA-binding domain or, alternatively, acts indirectly through its effect on the pro- tein ...mutant ...
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Structure-Function Analysis of Vaccinia Virus H7 Protein Reveals a Novel Phosphoinositide Binding Fold Essential for Poxvirus Replication
... H7 protein. Recombinant H7 protein was expressed and purified from ...H7 protein with a cysteine-to-serine sub- stitution at residue 89 (H7-C89S) yielded crystals of higher qual- ity, which gave rise ...
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Ligation of free HMGB1 to TLR2 in the absence of ligand is negatively regulated by the C-terminal tail domain
... the C-terminal tail; residues 1–185) were cloned into the pETM-11 vector and expressed in Escherichia coli strain BL21 (DE3) ...N- terminal histidine tag (his-tag) with a tobacco etch virus ...
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Membrane Binding Properties and Terminal Residues of the Mature Hepatitis C Virus Capsid Protein in Insect Cells
... and C-terminal ion series (21) ...core protein, we concluded that its sequence is S-T-N-P-K-P- Q-R and that its N terminus is blocked with an acetyl ...
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Structure-Based Mutational Analysis of the C-Terminal DNA-Binding Domain of Human Immunodeficiency Virus Type 1 Integrase: Critical Residues for Protein Oligomerization and DNA Binding
... the C-terminal, DNA-binding domain are not directly involved in phosphoryl transfer itself and therefore that it is likely that mutations in the C terminus will not affect disintegration ac- ...
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Mutation in the loop C terminal to the cyclophilin A binding site of HIV 1 capsid protein disrupts proper virus assembly and infectivity
... However, in contrast to the E187G and wild-type, we found that the E98A virions were non-infectious in per- missive CD4 positive H9 cells (Fig. 2B), despite being competent for particle assembly, normal processing of Gag ...
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Promyelocytic leukaemia protein isoform II and mild heat stress compromise human adenovirus type 5 gene expression
... type 1 immediate early protein ICP0 can disrupt the PML NB within a few hours post ...early protein synthesis and at the beginning of early protein ...PML protein expression, in ...
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... 32 protein (10). Hooking of the 17-residue C-terminal part of the adenovirus DBP to the nearest neighbor on the protein chain, around which the ssDNA is presumably wrapped, has been shown by ...
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Distinct and mutually inhibitory binding by two divergent β catenins coordinates TCF levels and activity in C elegans
... The C-terminal region of the POP-1 WBD (amino acids 422- 437) is predicted to form a stable ...WRM- 1 using this helix (will be termed the C-helix) in a manner similar to the ...
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C-Terminal Arginine Cluster Is Essential for Receptor Binding of Norovirus Capsid Protein
... protein was seen (Fig. 5A). Mass spectrometry analysis showed that these trypsin-released P polypeptides had an apparent molecular mass of 31.3 kDa (Fig. 5C; Table 3), indicating that trypsin also cleaved at the ...
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Multiple roles and therapeutic implications of Akt signaling in cancer
... 4E-binding protein 1; AGC, protein kinase A/protein kinase G/protein kinase C-like; Bcl-2, B-cell lymphoma 2; CAP, catabolite activator protein; CTMP, ...
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Resequencing at >=40 fold depth of the parental genomes of a Solanum Lycopersicum x S pimpinellifolium recombinant inbred line population and characterization of frame shift indels that are highly likely to perturb protein function
... chromosome 1, chromosome 4, and chromosome 8 with increased rates of ...chromosome 1 and chromosome 10, suggesting that the reference sequence contains a divergent introgression in these ...perturbed ...
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