C3H10T1/2
Effect of Androstenedione on Adipogenesis in Murine C3H10T1/2 Mesenchymal Cells
... Above observation led to the comparison of myogenesis and adipogenesis at 10 μM concentration of AD. At 10 μM concentration of AD, myogenesis was increased, but adipogenesis was not suppressed as expected for an androgen ...
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SIRT1 inhibits adipogenesis and promotes myogenic differentiation in C3H10T1/2 pluripotent cells by regulating Wnt signaling
... Wnt signaling pathways have important functions in controlling the fate of MSCs [4]. Wnt/β-catenin signaling promotes the differentiation of MSCs into myocytes and osteocytes and suppresses commitment to the adipocyte ...
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Effects of culture on PAMPS/PDMAAm double network gel on chondrogenic differentiation of mouse C3H10T1/2 cells: in vitro experimental study
... that C3H10T1/2 cells cul- tured on PAMPS/PDMAAm DN gels formed focal adhe- sions, aggregated rapidly and developed into large nodules within 7 days, while cells cultured on the polystyrene sur- face did not ...
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Dual effects and mechanism of TiO<sub>2</sub> nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
... TiO 2 nanotube surfaces was higher than that of the two control ...TiO 2 nanotube surfaces, especially on the surface of the 80 nm TiO 2 nano- tubes, were much lower than those of the two control ...
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Original Article Expression pattern and regulation of miR-19a and CCND1 in C3H10T1/2 chondrogenic differentiation
... Sox9 had been known as the master transcrip- tional factor and promoted expression of mark- er genes of aggrecan, col-IIa1/Xa1 in chondro- genic differentiation [16, 17]. We found in our pellets model, the gene ...
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Original Article Role of RUNX2 in osteogenic differentiation of mesenchymal stem cells induced by BMP9
... Remodeling of the skeleton is a continuous pro- cess during a human’s life. In order to replace damaged bone and meet the metabolic needs of the body, the remodeling process requires the coordination of bone resorption ...
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Yes associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells
... line, C3H10T1/2 clone 8) were purchased from the American Type Culture Collection (Manassas, VA, USA), and maintained in alpha minimum essential medium (αMEM) supplemented with 4 mM L-glutamine and 10% ...
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Transmission of ER stress response by ATF6 promotes endochondral bone growth
... To examine the expression of ATF6 and collagen X pro- tein in the course of chondrogenesis, total cell extracts pre- pared from micromass cultures of C3H10T1/2 cells in the presence of 300 ng/ml recombinant ...
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Effects of Hydrocortisone, Glycerophosphate and Retinol on the Differentiation of Mesenchymal Stem Cells and Vascular Endothelial Cells to Osteoblasts
... Finally, the effects of retinol on differentiation were examined. As shown in Figure 1, Figures 5-7, retinol exhibited a strong promoting effect on the differentiation of C3H10T1/2 MSCs and TKD2 VECs to ...
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Polycystin 1 interacts with TAZ to stimulate osteoblastogenesis and inhibit adipogenesis
... Next, we investigated the co-occupancy of endogenous Oc or aP2 promoter by PC1-CTT, TAZ, and Runx2 or PPARγ complex in C3H10T1/2 cells. OSE2 is a key osteoblast-specific cis-acting element in the proximal ...
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Azoramide, a novel regulator, favors adipogenesis against osteogenesis through inhibiting the GLP-1 receptor-PKA-β-catenin pathway
... assays, C3H10T1/2 and mouse bone marrow MSC-derived bone formation and adipogenesis assays, and specific staining for bone tissue and lipid droplets were used to evaluate the role of azoramide on the ...
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Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification
... (S)-HC and apatite/20α-HC coatings ( p >0.05). As the derivative of cholesterol, hydroxycholesterols are hydro- phobic, which are not easy to coprecipitate with apatite on Ti substrate [20] and also encounter with ...
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PubMedCentral-PMC4970218.pdf
... differentiation. C3H10T1/2 RhoA activity and -mediated functions were substrate specific such that M and N adherent cells were less sensitive to C3 inhibition of ...
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Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate
... that C3H10T1/2 cells primed by bovine chondrocyte-conditioned medium (CM) improved osteogenic responses like expressions of osteogenic gene marker like osteocalcin ( OCN ), alkaline phosphatase ( ALP ), ...
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Diclofenac and triamcinolone acetonide impair tenocytic differentiation and promote adipocytic differentiation of mesenchymal stem cells
... ultrastructure of collagen molecules [26] but also inhibit the regenerative properties of tendon stem cells [27]. Sur- prisingly, this evidence has not prevented the prevalence of injectable corticosteroids in routine ...
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Overlap in signaling between Smoothened and the α subunit of the heterotrimeric G protein G₁₃
... polycations. C3H10T1/2 cells were transduced with recombinant adenoviruses expressing LacZ (AdVLacZ) or GFP (AdVGFP) in the presence or absence of ...lysed 2 days later, and LacZ activity was ...
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Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells
... in C3H10T1/2 progeni- tors that resembled those seen in tenocytes (Figure ...in C3H10T1/2-BMP2 cells (Figure ...period C3H10T1/2-BMP2/Smad8 L+MH2 cells seemed to enter tenogenic ...
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Melatonin promotes the BMP9-induced osteogenic differentiation of mesenchymal stem cells by activating the AMPK/β-catenin signalling pathway
... bone metabolism by activating beta-catenin [29]. Mela- tonin can protect the osteogenic differentiation function of MSCs by promoting the activation of AMPK or β- catenin [20, 30]. In multiple sources of MSCs, BMP9 and ...
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Identifying deer antler uhrf1 proliferation and s100a10 mineralization genes using comparative RNA-seq
... studies, C3H10T1/2 stably transfected with s100a10 (Additional file 1: Figure S7) showed increased alp expres- sion ...addition, C3H10T1/2 stably transfected with s100a10 exhibited increased ...
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The alchemy of tendon repair: a primer for the (S)mad scientist
... from C3H10T1/2 cells, a murine cell line that recapitulates many features of normal multipotent mesenchymal cells (see the related article beginning on page ...
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