calu-3
Ipratropium is ‘luminally recycled’ by an inter play between apical uptake and efflux transporters in Calu 3 bronchial epithelial cell layers
... 16. Forbes, B.; Ehrhardt, C., Human respiratory epithelial cell culture for drug delivery applications. European journal of pharmaceutics and biopharmaceutics : 2005, 60 (2), 193-205. 17. Ong, H. X.; Traini, D.; Young, ...
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Puerarin transport across a Calu-3 cell monolayer – an in vitro model of nasal mucosa permeability and the influence of paeoniflorin and menthol
... in Calu-3 cells, the bidirectional transport of puerarin across Calu-3 cell monolayers, and the influence of drug compatibility on mucosal permeation in ...
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Deposition and transport of linezolid mediated by a synthetic surfactant Synsurf<sup>®</sup> within a pressurized metered dose inhaler: a Calu-3 model
... Figures 5–7 where the remaining concentration values are conveyed for all three pMDI preparations over all the three stages. Linezolid displays the highest concentration for remaining drug on the surface of the ...
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Apical Entry and Release of Severe Acute Respiratory Syndrome-Associated Coronavirus in Polarized Calu-3 Lung Epithelial Cells
... (2, 3, ...of Calu-3 monolayers allows the argument that the strong apical polarization expected to dominate the earlier days of infection will be breached, thus enabling the virus to intimately ...
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Clara cell protein mediates secretion of proteins, IL 8 and IL 6 in human airway epithelial cell line Calu 3 exposed to hyperoxia
... that Calu-3 cells secrete protein components associated with inflammation and innate immunity and identified pro-inflammatory cytokine IL-8 as a major cytokine mediator secreted by Calu-3 ...
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[HCO3-]-regulated expression and activity of soluble adenylyl cyclase in corneal endothelial and Calu-3 cells
... the 3'-untrans- lated region of the regulated mRNA contain a direct repeat of an eight-base AU sequence that functions as a pH- response element ...and Calu-3 ...
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Effect of budesonide in airway epithelial cell line Calu 3 with reduced CFTR function
... 14]. Calu-3 cells expresses high levels of functional CFTR, hence it has extensively been used in understanding the CFTR-mediated ion transport defect in CF [15- ...culture Calu-3 cells ...
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Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
... of Calu-3 cells to PLGA/PVA and PLGA/CS nanoparticles for 4 hours did not affect their viability as compared with untreated ...same Calu-3 cells, caused a 50% reduction in viability at a ...
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Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells
... SARS-CoV-infected Calu-3 cells (10), and that MERS-CoV in- fection resulted in extensive detachment of the monolayer within 24 h, an observation consistent with recent reports (13, ...infected ...
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A comparison of drug transport in pulmonary absorption models: isolated perfused rat lungs, respiratory epithelial cell lines and primary cell culture
... ranked Calu- 3 > NHBE >16HBE14o- (Table ...in Calu-3 and 16HBE14o- layers, but varied for the more lipophilic compounds in NHBE cell layers (Table ...
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Polystyrene nanoparticles activate ion transport in human airway epithelial cells
... all Calu-3 cell inserts were fed with complete fresh media for 1 hour and were washed for 30 minutes in Krebs–Henseleit solution (2 mL basolaterally, 500 µ L ...apically). Calu-3 cell mono- ...
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Measles Virus Infects both Polarized Epithelial and Immune Cells by Using Distinctive Receptor-Binding Sites on Its Hemagglutinin
... PC3) (Fig. 1A and data not shown). No signal for ZO-1 was observed on B-lymphoblastoid B95a cells, which are suscepti- ble to all MV strains. The subconfluent monolayers of these cell lines were infected with IC323-EGFP, ...
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Human cathelicidin, LL 37, inhibits respiratory syncytial virus infection in polarized airway epithelial cells
... ALI Calu-3, a 30 min wash of the apical sur- face of infected cells with EMEM + 10 % heat-inactivated fetal bovine serum was obtained 7 days pi, and cytokine and chemokine expression levels were determined ...
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Polystyrene nanoparticles activate ion transport in human airway epithelial cells
... Acute apical exposure of Calu-3 cells to N20 activated the short-circuit current in a concentration-dependent manner. Figure 1 shows the fitting of equation (1) to the experimen- tal data. The current ...
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TRAIL (CD253) Sensitizes Human Airway Epithelial Cells to Toxin-Induced Cell Death
... Surprisingly, Calu-3 cells did not secrete detectable levels of other “initiator” cytokines such as TNF- ␣ and IL-1 or the chemokine IL-8, even though each of their respective mRNA transcripts was ...
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The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures
... between Calu-3/PBMC and A549/PBMC co-cultures were also evi- dent in the inhibitor ...in Calu-3/PBMC co-cultures, suggesting IFN-γ signalling is not required for induction of ...
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Rescue of nonsense mutations by amlexanox in human cells
... the Calu-6 and 6CFSMEo- cell lines encode a nonfunctional truncated p53 or CFTR proteins, respect- ively, it was interesting to determine whether a treat- ment with amlexanox actually rescues the lost functions in ...
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Blocking of Exchange Proteins Directly Activated by cAMP Leads to Reduced Replication of Middle East Respiratory Syndrome Coronavirus
... to Calu-3 ...ESI-09-treated Calu-3 cells with an equal amount of infectious MERS-CoV (MOI of 20) in an ice bath for 2 h; cells were then washed thoroughly with ice-cold phos- phate-buffered ...
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Ablation of Nectin4 Binding Compromises CD46 Usage by a Hybrid Vesicular Stomatitis Virus/Measles Virus
... cells/filter. Calu-3 cells were grown in a liquid cover culture (LCC) so the culture medium was replaced in both the apical and basolateral chambers every other ...of Calu-3 cells between 14 ...
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Transport of the fluorescent organic cation 4 (4 (dimethylamino)styryl) N methylpyridinium iodide (ASP+) in human respiratory epithelial cells
... molecules. Their role in lung epithelium, however, is not well understood. OCT/N expression and activity was studied in cell lines of human alveolar (A549), bronchial (16HBE14o- and Calu-3) and intestinal ...
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