cAMP phosphodiesterase
Schizosaccharomyces pombe Adenylate Cyclase Suppressor Mutations Suggest a Role for cAMP Phosphodiesterase Regulation in Feedback Control of Glucose/cAMP Signaling
... of cAMP levels in a cgs2-s1 strain shows that these cells possess wild-type basal cAMP levels, but exhibit a dramatically increased glucose-stimulated re- sponse indicative of a loss of feedback ...Cgs2 ...
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The Dunce cAMP Phosphodiesterase PDE-4 Negatively Regulates Gαs-Dependent and Gαs-Independent cAMP Pools in the Caenorhabditis elegans Synaptic Signaling Network
... PDE-4 cAMP phosphodiesterase activity rather than indirect strong toxic effects of overproducing the PDE-4 protein, because neuronal overexpression of a pde-4d cDNA containing the inactivating ce268 [D448N] ...
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Suppression of mesangial proliferative glomerulonephritis development in rats by inhibitors of cAMP phosphodiesterase isozymes types III and IV
... sults strongly suggest that the proposed mitogen-activated sig- naling pathway and its “negative crosstalk” with cAMP signaling regulated by PDE-III, at the point of Raf-1 is most likely a dominant mechanism in ...
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The cAMP phosphodiesterase-4D7 (PDE4D7) is downregulated in androgen-independent prostate cancer cells and mediates proliferation by compartmentalising cAMP at the plasma membrane of VCaP prostate cancer cells
... of cAMP signalling cascades are an important factor in prostate cancer progression (Merkle and Hoffmann, ...messenger cAMP are governed by the processes of spatially distinct synthesis and degradation where ...
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Dimerization of cAMP phosphodiesterase-4 (PDE4) in living cells requires interfaces located in both the UCR1 and catalytic unit domains
... 2, 3, 4 has allowed us to identify a critical Arg173, Asn174 and Asn175 cluster in UCR1 PDE4D5 numbering whose mutation, to alanine, ablates dimer formation as long as the catalytic unit[r] ...
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Evolution of self organisation in Dictyostelia by adaptation of a non selective phosphodiesterase and a matrix component for regulated cAMP degradation
... opportunistic cAMP phosphodiesterase In contrast to the Dmin cAR, which can fully complement the aggregation deficient phenotype of a Ddis car-null mutant (Kawabe et ...for cAMP, which it shares with ...
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Sarcoplasmic reticulum associated cyclic adenosine 5' monophosphate phosphodiesterase activity in normal and failing human hearts
... reticulum-associated cAMP phosphodiesterase activity was examined in microsomes prepared from the left ventricular myocardium of eight heart transplant recipients with end-stage idiopathic dilated ...
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Genetic Interactions of the E3 Ubiquitin Ligase Component FbxA with Cyclic AMP Metabolism and a Histidine Kinase Signaling Pathway during Dictyostelium discoideum Development
... of cAMP would be low, but since RegA’s cAMP phosphodiesterase activity could depend on DhkA, eliminating DhkA would allow cAMP accumulation and restore ...the phosphodiesterase activity ...
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Cellular Action of Vasopressin in Medullary Tubules of Mice with Hereditary Nephrogenic Diabetes Insipidus
... cyclase, cAMP phosphodiesterase (cAMP-PDIE), as well as accumulation of cAMP in medullary collecting tubules (MCT) and in medullary thick ascending limbs of Henle's loop (MAL) microdissected ...
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A GENETICALLY DISTINCT FORM OF CYCLIC AMP PHOSPHODIESTERASE ASSOCIATED WITH CHROMOMERE 3D4 IN DROSOPHILA MELANOGASTER
... Effects of cations on cAMP hydrolysis: The CAMP phosphodiesterase activity present in homogenates of Basc/w+Y flies is a function of the Mg++ concentra- tion, as shown in[r] ...
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An Effect of Dexamethasone on Adenosine 3′,5′ Monophosphate Content and Adenosine 3′,5′ Monophosphate Phosphodiesterase Activity of Cultured Hepatoma Cells
... of cAMP was slightly increased and the increment produced by epinephrine plus theophylline was markedly ...on cAMP mediated processes may be due to an effect of these hormones on cAMP ...
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Theophylline accelerates human granulocyte apoptosis not via phosphodiesterase inhibition
... phosphodiesterase inhibitor), DMSO, and Hepes were purchased from Sigma Chemical Co. (St. Louis, MO). A specific phosphodi- esterase IV inhibitor (Rolipram; ME3167) was a gift from Meiji Seika Kaisha (Kawasaki, ...
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Intracellular Role of Adenylyl Cyclase in Regulation of Lateral Pseudopod Formation during Dictyostelium Chemotaxis
... natural cAMP waves generated by majority wild- type cells in mixtures (53, ...intracellular cAMP (24) and PKA is pre- sumably regulated by intracellular cAMP (37), these results suggested that ...
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Expression studies of recombinant cAMP-specific phosphodiesterase type 4
... About a decade ago, Liljestrom and Garoff, 1991 developed a m amm alian expression system based on Semliki Forest Virus (SPY). This system since its development has been used to express a large number o f recombinant ...
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cAMP/PKA/CREB/GLT1 signaling involved in the antidepressant-like effects of phosphodiesterase 4D inhibitor (GEBR-7b) in rats
... The hippocampus was dissected out bilaterally, frozen in liquid nitrogen, homogenized in ice-cold radioimmunopre- cipitation assay (RIPA) lysis buffer and diluted with 0.1 N HCl to a final protein concentration of 1 ...
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Elevated Cyclic AMP Levels in T Lymphocytes Transformed by Human T-Cell Lymphotropic Virus Type 1
... use cAMP as a ...Intracellular cAMP levels were measured by ...(D) cAMP was measured by ELISA and compared for Tesi and Tesi/Tet cells using a paired t test (P ⫽ ...cells, cAMP levels were ...
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Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors
... transient cAMP signals and/or by compartmentalized cAMP increases, as previously found for prostaglandin E1 (PGE1), which triggers transient in- creases in the cAMP concentration near the plasma ...
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Cyclic nucleotide phosphodiesterase 3A–deficient mice as a model of female infertility
... The PDE gene superfamily apparently encodes more than 50 PDE proteins (12). Some cells are relatively enriched in specific PDEs; these include oocytes (22, 23) and retinal rods and cones (12), in which PDE3A and PDE6, ...
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Mode of action of a Drosophila FMRFamide in inducing muscle contraction
... The application of 0.5 mmol l−1 IBMX, a broad-spectrum phosphodiesterase inhibitor A and 100 μmol l−1 Rp-cAMPS and 10 μmol l−1 Rp-8-pCPT-cGMPS, selective cAMP- and cGMP-dependent protein[r] ...
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Camp Coordination and Camp Management
... The teams were made up of personnel from the National Disaster Management Office (NDMO), the Vanuatu Humanitarian Team, the United Nations Disaster Assessment and Coordination (UNDAC) t[r] ...
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