cap-binding protein
Presence of the cap-binding protein in initiation factor preparations from poliovirus-infected HeLa cells.
... 2 Presence of the Cap-Binding Protein in Initiation Factor Preparations from Poliovirus-Infected HeLa Cells JOANNA HANSEN' AND ELLIE EHRENFELD1 2* Departments of Cellular, Viral and Mole[r] ...
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Association of cap-binding protein with eucaryotic initiation factor 3 in initiation factor preparations from uninfected and poliovirus-infected HeLa cells.
... 1982, p, 200-207 0022-538X/82/040200-08$02.00/0 Association of Cap-Binding Protein with Eucaryotic Initiation Factor 3 in Initiation Factor Preparations from Uninfected and Poliovirus-In[r] ...
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Poliovirus protease 3C (P3-7c) does not cleave P220 of the eucaryotic mRNA cap-binding protein complex.
... Consequently, it has been shown that the cap-binding protein CBP complex also termed eIF-4F or CBP II can restore translation of capped mRNAs in extracts from poliovirus-infected cells 3[r] ...
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Mutational Analysis of Plant Cap-Binding Protein eIF4E Reveals Key Amino Acids Involved in Biochemical Functions and Potyvirus Infection
... eIF4E- binding motif, YXXXXL ⌽ , where X represents any amino acid and ⌽ is a hydrophobic ...that binding of eIF4G or pep4G might affect the W94 fluorescence of lettuce ...
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Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection.
... Globin mRNA, which translated efficiently in mock-infected cell extracts lane 5 and did not translate in poliovirus-infected cell extracts, lane 7, as expected of a capped mRNA, could be[r] ...
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Translation by the adenovirus tripartite leader: elements which determine independence from cap-binding protein complex.
... Second, analysis of the secondary structure of the tripartite leader indicates that the 5' end is rather unstructured 49, which could possibly eliminate the requirement for eIF-4F activi[r] ...
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Sapovirus Translation Requires an Interaction between VPg and the Cap Binding Protein eIF4E
... eIF4E protein was isolated by m 7 -GTP Sepharose, whereas GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was not, confirm- ing the specificity of the assay ...VPg protein was incubated with ...
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The adenovirus tripartite leader may eliminate the requirement for cap-binding protein complex during translation initiation.
... The inhibition in translation of cellular and early adenovirus mRNAs following superinfection with wt poliovirus suggests that CBP complex is inactivated in these cells, presumably by po[r] ...
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Demonstration in vitro that eucaryotic initiation factor 3 is active but that a cap-binding protein complex is inactive in poliovirus-infected HeLa cells.
... CBP complex activity, determined in the presence of eIF3 from poliovirus-infected cells, is present in uninfected cells only and comigrates on sucrose gradient with an activity which res[r] ...
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Taking aim at translation for tumor therapy
... Increased cap-dependent mRNA translation rates are frequently observed in human ...the cap binding protein eukaryotic translation initiation factor 4E (eIF4E), leading to enhanced translation ...
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Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3.
... In combination with the reported mechanism involved in protein synthesis shutoff owing to cleavage of the cap-binding protein complex by the FMDV leader protease 14, this specific histon[r] ...
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Shutoff on Neuroblastoma Cell Protein Synthesis by Semliki Forest Virus: Loss of Ability of Crude Initiation Factors to Recognize Early Semliki Forest Virus and Host mRNA's
... Obviously, eIF-4B and cap-binding protein isolated from of neuThis study demonstrates that infection roblastoma cells by SFV results in a pronounced infected cells have the same biologic[r] ...
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Cowpea mosaic virus middle component RNA contains a sequence that allows internal binding of ribosomes and that requires eukaryotic initiation factor 4F for optimal translation.
... In vitro translation of both proteins directed by T7 transcripts of M-RNA was stimulated fourfold by eukaryotic initiation factor 4F eIF-4F, the cap-binding protein complex.. The ratio o[r] ...
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Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.
... Symbols: angled open box, cap-binding protein CBP complex eIF-4F and other initiation factors; shaded oval, a 40S ribosomal subunit; hatched circle, hypothetical factors which recognizes[r] ...
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Human transposons are an abundant supply of transcription factor binding sites and promoter activities in breast cancer cell lines
... human genes [8, 10]. The pervasive regulatory activity of LTRs can be explained by the fact that 85% of the LTR ret- rotransposons in the human genome consist of long ter- minal repeats only, which contain the original ...
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CAP protein superfamily members in Toxocara canis
... other CAP superfamily members [8] is absent, as is the Hinge-like sequence present in other mammalian CAP ...diverged” CAP subfamily in mammals, using the protein PRY1 from the yeast ...
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Binding of the Methyl Donor S-Adenosyl-l-Methionine to Middle East Respiratory Syndrome Coronavirus 2′-O-Methyltransferase nsp16 Promotes Recruitment of the Allosteric Activator nsp10
... Although cap 2=-O-MTases are present in different virus families (15), the exact role of this RNA-cap modification was only recently unraveled through reverse genetic studies performed, among others, on CoV ...
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Cap-Independent Translation Conferred by the 5′ Leader of Tobacco Etch Virus Is Eukaryotic Initiation Factor 4G Dependent
... 5⬘ cap, the binding site for ...a cap. In addition to the 5⬘ leader, a virus-encoded protein (Vpg) that is covalently attached to the 5⬘ terminus of TEV or turnip mosaic virus (a potyvirus ...
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HIV 1 Gag Blocks Selenite Induced Stress Granule Assembly by Altering the mRNA Cap Binding Complex
... tive binding of hypophosphorylated 4EBP1 to eIF4E on the 5= cap, which disrupts the eIF4F complex ...the binding of Gag to each of these host ...endogenous protein- protein interaction ...
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Comparative genomics of Butyrivibrio and Pseudobutyrivibrio from the rumen : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Microbiology and Genetics at Massey University, Palmerston North, New Zealand
... major protein/functional family types (Pfam, COG, TIGRfam and KO) was carried out and the results are displayed in a matrix format (Figures ...the protein/functional family ...
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