CAT, chloramphenicol acetyltransferase
Inhibition of calcitriol receptor binding to vitamin D response elements by uremic toxins
... Abbreviations used in this paper: CAT, chloramphenicol acetyltransferase; EMSA, electrophoretic mobility shift assay; GH, growth hormone; Nm..,, maximal binding capacity; N-UF, normal ul[r] ...
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A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA.
... B A CAT-specific protein with an Mr of 51,000 CAT-VP3 fusion indicated by the arrow is present in extracts of cells transfected with FLC/REP RNA lane 3 but is absent from mock-transfecte[r] ...
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Identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6.
... Effector plasmids containing these HHV-6 coding and 5' sequences were able to effect activation of heterologous promoter-chloramphenicol acetyltransferase CAT constructs, including adeno[r] ...
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Chloramphenicol acetyltransferase as a selection marker for chlamydial transformation
... the CAT gene as a selective marker ...a CAT gene was electroporated into EBs of ...both CAT mRNA and enzyme activity were detectable in the initial cultures of transformed chlamydiae, the authors ...
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Localization of sequences responsible for trans-activation of the equine infectious anemia virus long terminal repeat.
... 120 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We used the Escherichia coli chloramphenicol acetyltransferase gene cat to study sequences that influence expression[r] ...
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Negative regulatory element associated with potentially functional promoter and enhancer elements in the long terminal repeats of endogenous murine leukemia virus-related proviral sequences.
... 2746 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Three series of recombinant DNA clones were constructed, with the bacterial chloramphenicol acetyltransferase CAT g[r] ...
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The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure.
... Using constructs which direct synthesis of RNA containing a chloramphenicol acetyltransferase CAT gene between mutagenized influenza virus noncoding sequences derived from the NS genome [r] ...
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Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization.
... GB viruses A and C (GBV-A and GBV-C) are two recently described RNA viruses which appear to be members of the Flaviviridae. Although both viruses appear to contain long 5 * nontranslated regions, the sites of polyprotein ...
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Induction of CD4 expression and human immunodeficiency virus type 1 replication by mutants of the interferon-inducible protein kinase PKR.
... To further substantiate the activation of NF-kB by PKRLS9, the chloramphenicol acetyltransferase CAT reporter gene under the control of the wt HIV-1 LTR ptzIIICAT [6] or a mutant form of[r] ...
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Inhibition of TGF alpha gene expression by vitamin A in airway epithelium
... gene chloramphenicol acetyltransferase ...of CAT activity was observed ...inol, CAT activity was reduced by more than ...relative CAT activity when compared with the control ...
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In Vivo Effects of Mutations in Woodchuck Hepatitis Virus Enhancer II
... a chloramphenicol acetyltransferase (CAT) gene whose expression is dependent upon EnII, mutations in either the HNF-1 or the HNF-4 site strongly reduced CAT activity, while ablation of the ...
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Rescue of a synthetic chloramphenicol acetyltransferase RNA into influenza virus-like particles obtained from recombinant plasmids.
... strain. CAT activity in MDCK cell extracts was, however, unaffected by pretreatment of the COS-1 cells supernatant with (i) RNase A, (ii) an anti-NP MAb that inhibits the tran- scriptase activity associated with ...
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17 beta Estradiol inhibits expression of human interleukin 6 promoter reporter constructs by a receptor dependent mechanism
... of chloramphenicol acetyltransferase (CAT) reporter plasmid driven by the human IL-6 promoter was studied here in HeLa or murine bone marrow stromal cells (MBA ...stimulated CAT expression in ...
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Gattner, Michael (2019): Incorporation of pyrrolysine derivatives into proteins and development of bioorthogonal protein modification methods. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Supporting Figure 2: Biosynthetic conversion of 3 S -ethynyl- D -ornithine to ethynylpyrrolysine and incorporation into chloramphenicol acetyltransferase (CAT) using E..[r] ...
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Identification of the hepatitis B virus factor that inhibits expression of the beta interferon gene.
... The chloramphenicol acetyltransferase CAT-containing plasmids that were used in this study were i pTWU54, in which the cat gene is under the control of the 353-bp HindIll-HincIl DNA regi[r] ...
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Influenza virus NS1 protein stimulates translation of the M1 protein.
... M-CAT RNA, which contains the chloramphenicol acetyltransferase CAT reporter gene and the terminal noncoding sequence of segment 7 coding for the Ml and M2 proteins, was ribonucleoprotei[r] ...
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A reporter transgene indicates renal specific induction of tumor necrosis factor (TNF) by shiga like toxin Possible involvement of TNF in hemolytic uremic syndrome
... a chloramphenicol acetyltransferase (CAT) reporter gene that indicates TNF synthesis, CAT activity was induced within the kidney, but not in other ...
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The Replication Activity of Influenza Virus Polymerase Is Linked to the Capacity of the PA Subunit To Induce Proteolysis
... the chloramphenicol acetyltransferase (CAT) gene indicated that the CAT activity obtained correlated with the capacity of each PA mutant to induce ...
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The Hepatitis B Virus Core Promoter Is Strongly Activated by the Liver Nuclear Receptor Fetoprotein Transcription Factor or by Ectopically Expressed Steroidogenic Factor 1
... using chloramphenicol acetyltransferase reporter genes with the nucleocapsid promoter linked or not linked to enhancer I indicate that FTF is a potent activator of the HBV core promoter, more efficient than ...
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Mechanism of cAMP regulation of renin gene transcription by proximal promoter
... chloramphenicol acetyltransferase expression by cAMP, and indicated that the proximal promoter element from -75 to -47 (RP-2 element) overlapping the TATA-like region was able to confer cAMP ...
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