CD4+ and CD8+
Evaluation of immuno efficacy of a novel DNA vaccine encoding Toxoplasma gondiirhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model
... the CD4 + and CD8 + proportions in vaccinated mice were also increased significantly compared with that in mice of the three control groups (P < ...
8
Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay.
... Briefly, 50 m l of wash buffer was added to all assay wells. For each specimen, 25 m l of whole blood was added to each of three assay wells in positions designated by the manufacturer. Finally, the three assay wells ...
7
Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages
... for CD4; n = 10 for CD8; one CD8 sample was excluded as it failed to meet the internal control criteria); (3) HIV+ indi- viduals on HAART with detectable plasma viremia (VIR; n = 9 for CD4; n ...
13
Original Article Correlation analysis between urinary T lymphocyte subsets and lupus nephritis disease activity
... Abstract: Objective: In clinic, it’s significantly important to monitor lupus nephritis (LN) disease activity for the long- term prognosis of patients. To explore the role of urinary T lymphocyte true count in the ...
10
Treatment of encephalomyocarditis virus-induced central nervous system demyelination with monoclonal anti-T-cell antibodies.
... In order to determine the role of CD4+ and CD8+ T-cell subsets in the kinetics of EMC viral clearance, BALB/c mice were treated with 1 mg of either anti-CD4 GK1.5 or anti-CD8 2.43 monocl[r] ...
7
Protective efficacy of recombinant canine adenovirus type 2 expressing TgROP18 (CAV 2 ROP18) against acute and chronic Toxoplasma gondii infection in mice
... antigen-specific CD4 + T cells and CD8 + T cells responses in ...antigen-specific CD8 + T cell responses, including CTLs, which is a major mechanism of protection against intracellular ...that ...
10
The ORF3 Protein of Porcine Circovirus Type 2 Is Involved in Viral Pathogenesis In Vivo
... PCV2 replicates in the lymph nodes, lungs, and livers of infected pigs, followed by the impairment of the immune sys- tem by degradation of the lymphoid structures (1, 2, 15, 21, 23, 48), as well as changes in the ...
9
A new animal model of spontaneous autoimmune peripheral polyneuropathy: implications for Guillain-Barré syndrome
... both CD4 + and CD8 + cells in the genesis of EAN was confirmed by using CD4 and CD8 knock-out mice, where the severity of clinical scores and histopathological manis- festations of P0 peptide ...
14
ts1, a temperature-sensitive mutant of Moloney murine leukemia virus TB, can infect both CD4+ and CD8+ T cells but requires CD4+ T cells in order to cause paralysis and immunodeficiency.
... In some experiments, a specific subset of T cells was depleted in vivo by intraperitoneal administration of 0.5 mg of anti-CD4 for CD4+ T cells or anti-CD8 for CD8+ T cells antibodies on[r] ...
8
Divergent Kinetics of Proliferating T Cell Subsets in Simian Immunodeficiency Virus (SIV) Infection: SIV Eliminates the “First Responder” CD4+ T Cells in Primary Infection
... proliferating CD4 ⫹ T cells at between 8 and 10 days of infection, whereas mark- edly increased levels of proliferating CD8 ⫹ T cells were detected in the same tissues and at the same time points ...
7
Application of rapid in vitro co culture system of macrophages and T cell subsets to assess the immunogenicity of dogs vaccinated with live attenuated Leishmania donovani centrin deleted parasites (LdCen−/−)
... or CD8-FITC) were added to 2 μl/ ml of total lymphocytes and incubated at room temperature (RT) for 15 ...beled CD4 + or CD8 + cells were retained in the column, whereas the unlabeled cells ran ...
10
<p>Infiltrating CD4 and CD8 lymphocytes in HPV infected uterine cervical milieu</p>
... for CD4 and CD8 was ...for CD4 and CD8 were semi-quantitated by consensus of two pathologists who were blinded to the clinicopathological ...
9
Comparative Analysis of CD4+ and CD8+ T Cells in Tumor Tissues, Lymph Nodes and the Peripheral Blood from Patients with Breast Cancer
... lower CD4+ T cells and CD4+/CD8+ ratio compared with the micro-metastatic ...of CD8+ T cells was similar in the both dLN ...diminish CD4+ ...the CD4+/CD8+ ratio in dLN was ...
10
Apoptosis and Regeneration of Hepatocytes during Recovery from Transient Hepadnavirus Infections
... Grand Island, N.Y.) in a 20-l volume, with the reaction carried out at 42°C for 1 h. cDNAs were stored at ⫺20°C. For the amplification of T-cell markers and cytokines 1/20 of the cDNA solution was added to the PCR ...
11
Recombinant Vaccinia Virus-Induced T-Cell Immunity: Quantitation of the Response to the Virus Vector and the Foreign Epitope
... Recombinant vaccinia viruses expressing foreign genes from different pathogens have been extensively used in various ex- perimental studies and also in clinical trials (2, 20). Despite the widespread use of vaccinia ...
9
Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma
... both CD4+ and CD8+ T cells can recognize AFP epitopes presented by dendritic cells (DC) ...that CD8+ T cells from healthy donors can respond to four dominant and ten subdominant HLA- A * ...
16
Distribution of CD4<sup>+</sup> and CD8<sup>+</sup> exhausted tumor-infiltrating lymphocytes in molecular subtypes of Chinese breast cancer patients
... Patients and methods: A total of 133 patients with breast invasive ductal carcinoma were recruited consecutively from January 1, 2012 to December 31, 2013, and TILs were detected in H&E-stained sections. Expression ...
7
Desert hedgehog knockout mice show a reduced ratio of CD4+:CD8+ in thymic developing T cells
... of CD4+ cells was slightly increased in Dhh KO mice as compared to the WT ...of CD8+ thymocytes was slightly lower in Dhh KO mice relative to the WT mice (Figure ...both CD4+ and CD8+ ...
5
Homeostatic Cytokines Induce CD4 Downregulation in African Green Monkeys Independently of Antigen Exposure To Generate Simian Immunodeficiency Virus-Resistant CD8αα T Cells
... that CD4 downregulation can occur prior to anti- gen exposure has key implications for the diversity of the CD8 ␣␣ T cell pool and, consequently, the range of pathogens to which individual AGMs can ...The ...
11
Acute_HIV_Infection_and_CD4_CD8_Ratio.14.pdf
... for CD4 and CD8 alone may not take into account the level of immune dysfunction at ART initiation because pre-ART CD4/CD8 ratio remained strongly predictive of ratio ...pre-ART CD4 ...
9