cdna clone
Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks
... Non-infectious full-length cDNA clones of PVS-H95 First, a full-length cDNA clone pPVS-H-FL-AB was con- structed. Three amino acid substitutions, including Ser44Cys, Ile660Val, and Ser908Pro, due to ...
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Construction of a Severe Acute Respiratory Syndrome Coronavirus Infectious cDNA Clone and a Replicon To Study Coronavirus RNA Synthesis
... full-length cDNA clones (3, 6, 26, 27, 32–34) and self-replicating RNAs, or rep- licons (2, 28, ...bacteria, cDNA clones and replicons have been engineered using bacterial artificial chromosomes (BACs) (3), ...
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Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.
... astroviral cDNA clone described here should prove useful for defining the minimal and optimal requirements for the use of astrovirus as an expression vector, and for developing a packaging system for vector ...
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Construction of an Infectious cDNA Clone of Aichi Virus (a New Member of the Family Picornaviridae) and Mutational Analysis of a Stem-Loop Structure at the 5′ End of the Genome
... virus cDNA sequence with the 5⬘-end sequence published ...length cDNA clone with the novel 5⬘-end sequence was constructed as ...a cDNA clone obtained by 5⬘-RACE as a ...A clone ...
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Development and Characterization of an Infectious cDNA Clone of the Modified Live Virus Vaccine Strain of Equine Arteritis Virus
... infectious cDNA clone of the current MLV vaccine strain (ARVAC) of EAV was ...full-length cDNA clone was noninfectious due to a single nonsynonymous nucleotide substitution in the Nsp3 coding ...
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Correlation between Point Mutations in NS2 and the Viability and Cytopathogenicity of Bovine Viral Diarrhea Virus Strain Oregon Analyzed with an Infectious cDNA Clone
... To obtain a mutant for which the probability of reversion is lower, we decided to delete the S at position 1555 within the sequence of BVDV Oregon. Interestingly, after expression of the respective mutant polyprotein ...
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Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
... We also demonstrated the feasibility of using an infectious cDNA clone for the analysis of RV RNA replication and gene expression. We were able to detect the synthesis of minus- or plus-strand RNA in Vero ...
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Nucleotide sequence of a cDNA clone encoding the entire glycoprotein from the New Jersey serotype of vesicular stomatitis virus.
... 1 0022-538X/83/040162-08$02.00/0 Copyright 0 1983, American Society for Microbiology Nucleotide Sequence of a cDNA Clone Encoding the Entire Glycoprotein from the New Jersey Serotype of [r] ...
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Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2
... Tonga/74 cDNA clone cDNA fragments of DEN-2 Tonga/74 were generated by reverse-transcription of the genome as indicated in Figure ...cloned cDNA fragments spanning the ...full-length ...
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Characterization of a Pathogenic Full Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A
... full-length cDNA viral genome from a set of sequen- tially designed smaller cDNAs ...molecular clone for PEDV, the highly virulent PC22A strain ...full-length cDNA with- out alteration of the viral ...
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Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721
... full-length cDNA clone for hypovirus CHV1-EP721, and mapping of determinants in the viral genome responsible for the extremely low level of viral dsRNA ...
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Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
... infectious cDNA clone of the viral genome. Infectious cDNA clones of several enteroviruses have been successfully constructed, includ- ing poliovirus, EV71 [14], CA16 [15], CA6 [16], CB3 [17], ECHO30 ...
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In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release.
... 4107-4113 0022-538X/91/084107-07$02.00/0 Copyright © 1991, American Society for Microbiology In Vitro Mutagenesis of a Full-Length cDNA Clone of Semliki Forest Virus: the Small 6,000-Mol[r] ...
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A Full Length Infectious cDNA Clone of Zika Virus from the 2015 Epidemic in Brazil as a Genetic Platform for Studies of Virus Host Interactions and Vaccine Development
... infectious cDNA clone of ZIKV isolated from the 2015 epidemic in ...The cDNA-derived ZIKV replicated efficiently in a variety of cell lines, including those of both neuronal and placental ...of ...
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The preparation of an infectious full length cDNA clone of Saffold virus
... full-length cDNA clone of JPN08-404 by using the specific primers carrying a T7 promoter as described in Materials and ...full-length cDNA clone was designated pSAF404 (Figure ...
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Recovery of a Neurovirulent Human Coronavirus OC43 from an Infectious cDNA Clone
... MluI, and AsiSI restriction sites, respectively. These same regions also contained a mutagenized SfoI site (p), whereas regions D and E contained the original SfoI site, located at position 18585 of the viral genome. ...
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Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus (GALV Nf) and its biological activity in Nicotiana benthamianaand grapevine plants
... The inoculation of grapevine plants with GALV has never been reported [1,6]. Having established that the GALV-Nf cDNA clone induces systemic infection in N. benthamiana plants, we tested the same construct ...
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The In Vitro-Synthesized RNA from a cDNA Clone of Hepatitis E Virus Is Infectious
... Hepatitis E virus (HEV) is an important etiological agent of epidemic and sporadic hepatitis, which is endemic to the Indian subcontinent and prevalent in most of the developing parts of the world. The infection is often ...
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Infectious cDNA Clone of the Epidemic West Nile Virus from New York City
... full-length cDNA clones of flaviviruses. (i) Full-length cDNA clone can be assembled through in vitro ligation of cDNA ...Genome-length cDNA containing an upstream promoter for ...
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Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
... Fungal strains, growth conditions, and phenotypic measurements. C. para- sitica EP155 (ATCC 38755), a virulent hypovirus-free strain, was provided by S. Anagnostakis (Connecticut Agricultural Experiment Station), who ...
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