cDNA microarrays
Observation of intermittency in gene expression on cDNA microarrays
... a cDNA microarray which has been spotted with DNA targets that are complimentary to the ...mary, cDNA microarrays use competitive hybridization to compare concentration levels of thousands of genes ...
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Identification of ‘stress and Ca++ metabolism related gene expression’ in wild Peromyscus exposed to environmental lead using combinatorial data from Mus cDNA microarrays and blood metabolites
... Mus cDNA microarrays in ab- sence of suitable Peromyscus genetic ...these microarrays were successfully used in gene expression studies in an even more remotely related rodent, the arctic ground ...
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Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays
... using microarrays allows a more comprehensive picture of the host response during infec- tion with ...Using cDNA microarrays Moser and co-workers found 307 anonymous transcripts in blood leukocytes ...
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Identification of new participants in the rainbow trout (Oncorhynchus mykiss) oocyte maturation and ovulation processes using cDNA microarrays
... decades. However, the use of nylon cDNA microarrays is not very common in comparison to glass slide microarray technology. Nevertheless, this technology has successfully been used for several years [27,35]. ...
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Physical mapping of genes in somatic cell radiation hybrids by comparative genomic hybridization to cDNA microarrays
... SSC. cDNA microarrays were fabricated as described ...human cDNA (ESTs) clones in a 96-well format from DNA minipreps (Qiagen) using modified M13 universal ...The cDNA microarrays ...
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Use of cDNA Microarrays To Monitor Transcriptional Responses of the Chestnut Blight Fungus Cryphonectria parasitica to Infection by Virulence-Attenuating Hypoviruses
... Hypoviruses are a family of cytoplasmically replicating RNA viruses of the chestnut blight fungus Cryphonec- tria parasitica. Members of this mycovirus family persistently alter virulence (hypovirulence) and related ...
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Whole-transciptome analysis of [psi+] budding yeast via cDNA microarrays
... of elucidating potential genomic pressures as a result of the prion state. To achieve this goal a data analysis pipeline inclusive of raw-image processing through expression data meta-analysis was developed and validated ...
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Expression of Respiratory Syncytial Virus-Induced Chemokine Gene Networks in Lower Airway Epithelial Cells Revealed by cDNA Microarrays
... Oligonucleotide-based microarrays of RSV-infected bron- chiolar cells. SAE cells are well-differentiated primary cells obtained from normal human bronchioli (52). By immuno- histochemistry, SAE cells are positive ...
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Functional Genomics Investigation of Microbial Physiology in the Hyperthermophilic Microorganisms Pyrococcus furiosus and Thermotoga maritima
... Full genome cDNA microarrays were used to assess differential gene expression patterns in the hyperthermophilic archaeon Pyrococcus furiosus grown on a range of glucose- based carbohydr[r] ...
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Microarrays and molecular markers for tumor classification
... used cDNA microarrays and hierarchical clustering analysis to explore whether breast-cancer patient samples could be grouped on the basis of distinguishing patterns of gene ...
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Identification of Schistosoma mansonigender associated gene transcripts by cDNA microarray profiling
... Even less is known about the expression of male-associated transcripts, although these molecules clearly exist [12]. As female worms of some Schistosoma species (including S. mansoni) are incapable of reaching sexual ...
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Comparative gene expression profiles for highly similar aggressive phenotypes in male and female cichlid fishes (Julidochromis)
... Julidochromis marlieri and Julidochromis transcriptus are two closely related Tanganyikan cichlids that have evolved different behavior and mating strategies since they diverged from their common ancestor. While J. ...
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Signal stability of Cy3 and Cy5 on antibody microarrays
... protein microarrays is scant. Although repeated scans of cDNA microarrays have been conducted [30-35], it is not known whether amine-cou- pled fluorescent dyes on antibody/protein microarrays ...
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High throughput genomic technology in research and clinical management of breast cancer Molecular signatures of progression from benign epithelium to metastatic breast cancer
... by cDNA microarrays between normal cells, primary invasive carcinoma and metastatic cells, Mimori and coworkers [22] were able to detect genes directly associated with each tumor stage in tumor development ...
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Application of sector protein microarrays to clinical samples
... Reverse phase protein microarrays were printed with whole cell protein lysates as described by Paweletz et al (Oncogene 2001) (12). Briefly, the lysates were printed on glass backed nitrocellulose sector array ...
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MRP8, A New Member of ABC Transporter Superfamily, Identified by EST Database Mining and Gene Prediction Program, Is Highly Expressed in Breast Cancer
... various cDNA libraries. Because each cDNA clone is gener- ated from a transcript, the frequency and distribu- tion of the many different transcripts in any given tissue depend on the tissue-specific activity ...
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"Hook"-calibration of GeneChip-microarrays: Theory and algorithm
... probe"-property. Microarrays of the GeneChip- type use so-called probe sets of several probes (usually N set = 11) for estimating the expression of each considered ...
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The promise of microarrays in the management and treatment of breast cancer
... A few studies have used new genomic approaches for the study of inherited breast cancer (reviewed in [8]). There is accumulating evidence, both epidemiological and histo- logical, that tumors arising as a result of ...
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Microarrays, deep sequencing and the true measure of the transcriptome
... D. pseudoobscura. (a) Results for female heads; (b) results for male heads. We used custom designed Nimblegen arrays to an early release of the D. pseudoobscura annotation. This array consists of 50-mer probes selected ...
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A CDNA selection approach to isolate Y-linked genes expressed in testis
... labeled cDNA as probe gave a single hybridizing band for each enzym e digest (Fig ...respective cDNA sequence as one continuous stretch of sequence and this was identical to that o f the corresponding ...
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