Cell-free DNA levels
Decreased serum cell-free DNA levels in rheumatoid arthritis
... cfDNA levels in RA may at least in part be explained by the use of different techniques (for example, qPCR, ELISA or UV-spec- trophotometry) and the different DNA targets chosen to perform the ...
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Prognostic utility of admission cell-free DNA levels in patients with chronic obstructive pulmonary disease exacerbations
... Serum samples were drawn from 16 COPD outpatients (64 ± 9 years, 10 females), who were seen consecutively in clinic at the Pulmonology Institute, Tel Aviv Sourasky Medical Center, and met the GOLD criteria for COPD. ...
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Levels of circulating insulin cell-free DNA in women with polycystic ovary syndrome – a longitudinal cohort study
... The cell-free DNA methylation pattern of the insulin gene promoter has been suggested as a marker of pancreatic islet β-cell destruction, and thereby as an early marker of ...insulin ...
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Cell-free DNA in non-small cell lung cancer
... plasma DNA might repre- sent a new approach to the monitoring of surgical procedures or to the assessment of the efficacy of ...plasma DNA quantification by re- al-time quantitative PCR in the entire cohort ...
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Cell-free circulating tumor DNA in cancer
... Challenges of ctDNA analysis on the path to clinical utility As a noninvasive “liquid biopsy,” ctDNA is a promising bio- marker that provides highly specific and complementary information in the diagnosis, prognosis, and ...
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Branched DNA-based Alu quantitative assay for cell-free plasma DNA levels in patients with sepsis or systemic inflammatory response syndrome
... cf-DNA levels positively correlated with previous indicators such as CRP and PCT and yielded compara- ble or better sensitivity and speci ficity compared to these ...
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Circulating cell-free tumour DNA in the management of cancer
... far. Levels of ctDNA correspond with the clinical course and ctDNA increases with disease progression and correspondingly decreases with response to ...disease free at a median of ...
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Circulating Cell Free DNA in the Diagnosis of Trophoblastic Tumors
... analyzed the ctDNA was indistinguishable from that of the patient. This was con firmed in one case (CFD 017) in which genotyping of the tumor tissue, from a subsequent biopsy, showed the tumor to be non- gestational with ...
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Tumor-Specific Mitochondrial DNA Variants Are Rarely Detected in Cell-Free DNA
... (squares), levels of CA 15-3 (triangles), and ratio of mtDNA:nDNA molecules (asterisks) on log-scale in the cfDNA samples (vertical) at five time points ...
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Original Article Serum cell free DNA by branched DNA in patients with gastric cancer
... The presence of cf-DNA in the human blood- stream was first described in 1948 by Mandel and Metais. Tan et al. demonstrated the pres- ence of DNA in the serum and plasma of patients with systemic lupus ...
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A cell-free replication system for human polyomavirus JC DNA.
... 5). Replication of a DNA that lacked TAg-binding site I (pJC433 D 112) showed lower levels of DNA synthesis than the full-length construct. In the presence of 0.25 m g of TAg, the FIG. 1. ...
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Plasma cell free DNA in patients needing mechanical ventilation
... plasma cell-free DNA over the best cutoff value at admission, present infection, and all variables found to be independent predictors for 90-day mortality in the FINNALI study (SAPS II score minus ...
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Cell-free DNA as a diagnostic marker for cancer: current insights
... of patients with advanced pancreatic, ovarian, colorectal, bladder, gastroesophageal, breast, melanoma, hepatocellu- lar, and head-and-neck cancers presented evaluable ctDNA. However, ctDNA was found only in 48%–73% of ...
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The Prognostic Value of Cell Free DNA in Advanced Non Small Cell Lung Cancer
... Fournie et al. also found a positive correlation between high levels of cfDNA and poor prognosis, but the study was biased by the mixing of NSCLC and SCLC [19]. In an attempt to address these problems, we ...
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Changes in circulating cell free nuclear DNA and mitochondrial DNA of patients with adolescent idiopathic scoliosis
... fusion levels were also highly difficult to decide because of its com- plexity as well as uncertainty of ...expression levels of ACTB and GAPDH in different tissues of scoliotic patients might be used to ...
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Cancer gene mutation detection in circulating cell free DNA in blood
... The GeneFirst EGFR and KRAS PCR assays demonstrated good levels of sensitivity at certain points during the investigation, although this varied greatly as the assay versions changed. The best examples are the LoD ...
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Prognostic utility and characterization of cell free DNA in patients with severe sepsis
... cfDNA levels among patients with Gram-positive, Gram-negative, fungal, mixed, or unknown ...cfDNA levels and renal ...nucleosome levels, microor- ganisms, and renal function in patients with systemic ...
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Circulating Cell-Free Tumour DNA for Early Detection of Pancreatic Cancer
... tumour DNA (ctDNA) as a non-invasive biomarker in the early detection of pancreatic ...ctDNA levels in blood correlate with disease progression but its widespread application for early PDAC detection ...
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Circulating Cell-Free DNA in Hepatocellular Carcinoma: Current Insights and Outlook
... In the context of precision medicine, one of the biggest chal- lenges in HCC clinical management is the frequent lack of tumor tissue for molecular profiling. Tumor materials may be obtained during resection and/or ...
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Methylated cell-free DNA profiles of patients with pancreatic ductal adenocarcinoma
... Summary of ddPCR input cfDNA concentrations in the literature................................................................................. Patient demographics for genetic screening.......................... ...
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