cell-free extract
The effect of NaCl substitution with KCl on proteinase activities of cell-free extract and cell-free supernatant at different pH levels and salt concentrations: Lactobacillus acidophilus and Lactobacillus casei
... of cell-free extract and cell-free supernatant of the probiotics Lac- tobacillus acidophilus and Lactobacillus ...ed cell pellets were used to prepare cell-wall ...
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Chromate reductase activity in whole cells and crude cell free extract of Acinetohacter Haemolyticus
... Extensive use of hexavalent chromium, (Cr(VI)) in various industrial applications is a threat to human health, living resources and ecological system due to its high solubility, toxicity and carcinogenic effects. ...
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Translation of Virus mRNA: Synthesis of Bacteriophage Qβ Proteins in a Cell-Free Extract from Wheat Embryo
... Translation of Virus mRNA: Synthesis of Bacteriophage Q,B Proteins in a Cell-Free Extract from Wheat Embryo Received for publication 24 August 1973 The RNA from bacteriophage QB can be t[r] ...
8
Flexibility vs robustness in cell cycle regulation of timing of M phase entry in Xenopus laevis embryo cell free extract
... In conclusion, the data presented in this paper confirm that our simple mathematical model accurately emulates experimen- tal results showing that the interplay between CDK1 activation and PP2A inactivation determines ...
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Authentic Replication and Recombination of Tomato Bushy Stunt Virus RNA in a Cell-Free Extract from Yeast
... the cell extract. A major advantage of the yeast-based cell-free tombusvirus replication assay is that it can be used to charac- terize important biochemical features of replicase complex for- ...
14
Escherichia coli based cell free extract development for protein based cancer therapeutic production
... temperature for 90 days and ~ 30% of its activity after storage at 4ºC for one year (Smith et al., 2014a). This facilitates facile trans- portation by avoiding ultralow temperature required for long-term storage of ...
8
MPF, starfish oocyte and cell free extract in the background an interview with Takeo Kishimoto
... Thanks a lot. Certainly, to my greatest delight, we have at last resolved the three decades-long enigma that was born in our Dev. Biol. paper of 1981 (for review, Kishimoto 2015). As I mentioned earlier, the question had ...
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In Vitro Anti-Mycotoxigenic and Anti-Aflatoxigenic Properties of Probiotic Bacteria; Lactobacillus plantarum and L. paracasei
... The anti-fungal action of the metabolites produced by the probiotics was further demonstrated using a disc diffusion assay by treatment of the A. parasiticus culture with the discs loaded with cell-free ...
9
Cellular oxido-reductive proteins of Chlamydomonas reinhardtii control the biosynthesis of silver nanoparticles
... reinhardtii cell free extract (in vitro) and in vivo cells mediated synthesis of silver nanoparticles reveals SNPs of size range 5 ± 1 to 15 ± 2 nm and 5 ± 1 to 35 ± 5 nm ...
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The identification and functional characterisation of caspase substrates involved in inflammation
... Initially we adapted a cell-free extract model to specifically activate or inhibit the inflammatory caspases and then resolved control and inflammatory caspase activated proteomes on two[r] ...
222
Design of a biotransformation process for dehalogenation of chlorinated compounds
... weaken cell walls and aid protein release (Miyashiro et ...The cell suspension was then passed through a French pressure cell five times at ...the cell free extract was ...
232
Antioxidative potential of folate producing and cholesterol removing probiotic Lactobacillus species
... for cell free extract (CFE), intact cells (ICs), heat killed cells (HKCs) and intracellular cell free extract (ICFE) of lactobacilli strains using various antioxidant assays, ...
7
Camel Milk: A Potential Source of Therapeutic Lipolytic Lactic Acid Bacteria
... Lipolytic activity of crude lipase, obtained from isolated lactic acid bacteria, was determined by agar well diffusion method (Blake et al, 1996). The basic principle behind this method is the hydrolysis of lipid ...
6
Synthesis of an antiviral drug precursor from chitin using a saprophyte as a whole-cell catalyst
... in cell-free extracts of the recombinant strain, according cell-free extracts obtained from the cultivation in a bioreactor on chitin (vide infra) were spiked with NeuNAc and incu- bated for ...
9
DNA binding properties of simian virus 40 T-antigens synthesized in vivo and in vitro.
... Premixing the labeled reticulocyte cell-free products with the same amount of infected-cell extract normally used in a DNA binding experiment, representing a great excess over that synth[r] ...
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Protective effect of aqueous extract from Spirulina platensis against cell death induced by free radicals
... aqueous extract of Spirulina induced apoptosis in hepatic stellate cells ...the cell cycle of HSC by the extract may not be due its antioxidant ...the cell cycle pro- tein cyclin D1. Spirulina ...
8
Dualistic Properties of Cosmetic Formulations Based on Phenylpropanoids from Ajuga reptans
... the extract was introduced win different cosmetic formulations, and subjected to a series of radia- tion intensity and energy equal to that of the sun, to ver- ify the possible ...the extract, in order to ...
9
CHARACTERIZATION AND APPLICATIONS OF SILVER NANOPARTICLES SYNTHESIZED FROM SYZYGIUM CUMINI
... The present study exhibit a simple environmentally beginning method of synthesis of silver nanoparticles from stem bark S. cumini. This method can be further used for industrial production of nanoparticles at room ...
12
Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela tionship with Antioxidant Activity
... this extract as it is well kno- wn that, Con A induced oxidative stress in ...liver cell injury, antioxidant like ellagic acid are capable of prote- cting hepatocytes from VK3- or ConA-induced liver da- ...
10
Cell-free fat extract promotes tissue regeneration in a tissue expansion model
... To help the skin expand, many studies have ap- plied drugs, such as anti-contractile agents and dimethyl sulfoxide. However, these drugs can elicit additional complications [14, 15]. Recently, stem cell therapy ...
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