cell-free synthesis
Cell-free synthesis of functional phospholipase A1 from Serratia sp.
... the cell-free synthesis reaction mixture under the condi- tions described in “Methods” ...the cell-based expression, approximately ...for cell-free ...the cell-free ...
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Cell-Free Synthesis of Encephalomyocarditis Virus
... tions (Fig. 2C), in sharp contrast to the broad range observed for translation. Lowering the magnesium salt concentration to 2 mM resulted in almost no RNA synthesis (Fig. 2C) while causing only marginal ...
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Cell-free synthesis of herpes simplex virus-coded pyrimidine deoxyribonucleoside kinase enzyme.
... 0 min 0.1 This strongly suggests that in vitro synthesis of 90 min 19.7 active HSV-specified thymidine kinase occurs 3.4 in the cell-free system, and furthermore dem- Incubation at 4°C i[r] ...
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Cell-free synthesis of polyoma virus capsid proteins VP1 and VP2.
... DISCUSSION In the work described here we have established that proteins closely related, if not identical, to polyoma virus capsid proteins VP1 and VP2 can be made in a wheat germ cell-f[r] ...
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Cell-free synthesis of simian virus 40 T-antigens.
... In addition to the data presented here, strong evidence in support of the model for the location of sequences coding for the SV40 T-Ags has recently come from experiments which have show[r] ...
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Coronavirus JHM: Cell-Free Synthesis of Structural Protein p60
... The virus was propagated twice in suckling mouse brain, isolated, plaque purified four times on L929 cells, and then grown in Sac- cells at low multiplic10 Downloaded from http://jvi.asm[r] ...
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Cell-free synthesis and assembly of vesicular stomatitis virus nucleocapsids.
... Results presented in this communication show that VSV proteins synthesized in vitro concurrently with genomic RNA assembled into structures that i banded in CsCl gradients or eluted from[r] ...
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Cell-free synthesis of herpes simplex virus proteins.
... The protein is precipitated only from in vivo extracts prepared from cells infected with wild-type or 20R1 TK revertant virus a revertant derived from mutant 20 TK- or from in vitro reac[r] ...
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Synthesis of Capsid and Noncapsid Viral Proteins in Response to Encephalomyocarditis Virus Ribonucleic Acid in Animal Cell-Free Systems
... From such a comparison, Smith, Marcker, and Mathews found no evidence for the cell-free synthesis of virion capsid proteins, but their data did indicate some identity between the tryptic[r] ...
9
Studies on rabbit reticulocyte ribosomes and polyribosomes and their relation to hemoglobin synthesis
... For this reason the dissertation is divided into three sections: Part I on ribosomes in cell-free synthesis systems derived from reticulocytes, Part II on hemoglobin synthesis in cells i[r] ...
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Unilateral Synthesis of Reovirus Double-Stranded Ribonucleic Acid by a Cell-Free Replicase System
... A "hybridization-competition" experiment in which the replicase product was denatured and annealed in the presence of an excess of plus-RNA indicated that the in vitro replicase reaction[r] ...
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The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver
... Необходимо отметить, что после удаления ДМСО уровень биосинтеза протеинов не только возвращается к прежним значениям, но и превышает их на 33%, что, возможно, связано с влиянием дан[r] ...
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Solvent-Free Microwave Synthesis of Trialkylphosphates
... The first synthesis step consists in to add successively in the reactor: the alcohol, the phosphorus oxychloride in the heptane and the pyridine. The addition operation was realized between 0 to 10°C and ...
5
BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES OF ADIANTUM CAPILLUS VENERIS L AND THEIR EVALUATION OF ANTIBACTERIAL ACTIVITY AGAINST HUMAN PATHOGENIC BACTERIA
... nanoparticles in aqueous colloid solution are conformed using UV-VIS spectral analysis. As the Adiantum capillus-veneris extract with mixed with aqueous solution of silver nitrate, it started to change the colour ...
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Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies.
... To obtain preliminary information on the synthesis of viral polypeptides in cells infected with cell-free virus, the infected cultures and uninfected control cultures were labeled at var[r] ...
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POTENTIAL HERBS AGAINST DIABETES MELLITUS – AN UPDATE
... CONCLUSION: Diabetes mellitus is a syndrome, initially characterized by loss of glucose homeostasis resulting from defects in insulin secretion, insulin action both resulting in impaired metabolism of glucose and other ...
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Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.
... The polypeptide molecular weight, 56,000, synthesized in a cell-free reaction, that comigrates with authentic NP has a tryptic peptide pattern identical to the tryptic peptides of NP fro[r] ...
5
Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia
... In the present investigation, a cell free system for protein synthesis has been developed from human reticulocytes and this latter hypothesis tested further by comparing the capacity for[r] ...
8
Cell-free enzymatic synthesis of GDP-l-fucose from mannose
... ManB and ManC to the reaction, followed by addition of Gmd and then WcaG to overcome feedback inhibi- tion of the end-product on Gmd. GDP-L-fucose was produced at 178.6 mg/l with a conversion rate of 14.1%. This 3-step ...
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Synthesis of the small subunit of ribulose 1,5 bisphosphate carboxylase
... If the signal hypothesis accounts for the transport of proteins into chloroplasts, specific features of this aspect of protein synthesis w o u l d be expected. Either the chloroplast envelope would bear polysomes ...
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